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Transcriptomic “portraits” of canine mammary cancer cell lines with various phenotypes

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Abstract

In light of the high incidence of mammary cancer in dogs and completion of the canine genome sequencing, the new possibilities of gene profiling by using DNA microarrays give hope to veterinary oncology. The cell lines isolated from mammary tumors are a valuable tool in developing and testing new pathway-specific cancer therapeutics. Differential cytometric analysis of 6 canine mammary cancer cell lines was performed. We divided cell lines into 3 groups based on their phenotype: 2 lines with high proliferative potential, 2 lines with high antiapoptotic potential, and 2 lines with high metastatic potential. DNA microarray analysis revealed common genes for cell lines of each group. We found that genes encoding the receptors for growth hormone and ghrelin are related to high proliferation rate, whileABR (active BCR-related) andTMD1 (TM2 domain containing 1) genes are related to a high antiapoptotic potential of the cancer cells. Metastatic properties of mammary cancer cells seem to be associated with elevated expression ofPGP (P glycoprotein),SEMA3B (semaphorin 3B), andSTIM1 (stromal interaction molecule 1).

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Authors and Affiliations

  1. Department of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences (SGGW), Nowoursynowska 159, 02-776, Warsaw, Poland

    M. Król, K. M. Pawłowski, A. Majewska & T. Motyl

  2. Cell Biology Department, National Medicines Institute, Warsaw, Poland

    J. Skierski

  3. Department of Internal Medicine, University of Giessen Lung Center, Justus Liebig University, Giessen, Germany

    P. Turowski & R. E. Morty

  4. Department and Clinic of Obstetrics, Ruminant Diseases and Animal Health Care, Wrocław University of Environmental and Life Sciences, Wrocław, Poland

    J. Polańska

  5. Department of Biochemistry, Pharmacology and Toxicology, Wrocław University of Environmental and Life Science, Wrocław, Poland

    M. Ugorski

  6. Department Immunochemistry, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Wrocław, Poland

    M. Ugorski

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  1. M. Król
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  2. K. M. Pawłowski
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  3. J. Skierski
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  4. P. Turowski
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  5. A. Majewska
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  6. J. Polańska
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  7. M. Ugorski
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  8. R. E. Morty
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  9. T. Motyl
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Correspondence to T. Motyl.

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Król, M., Pawłowski, K.M., Skierski, J. et al. Transcriptomic “portraits” of canine mammary cancer cell lines with various phenotypes. J Appl Genet 51, 169–183 (2010). https://doi.org/10.1007/BF03195725

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  • DOI: https://doi.org/10.1007/BF03195725

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