Abstract
The microbial production of ethanol and other solvents from renewable biomass is an attractive alternative to fuels and basic chemical feedstocks. Considerable efforts have been made in the past 10 years to improve the production of altenative fuel chemicals by various biological systems. Much current interest is focussed on the processes based on cellulosic and hemicellulosic feedstocks, the hydrolyzates of which contain a complex mixture of sugars. The technology to convert hexoses to ethanol is well established, however, conversion of pentoses and other sugars poses problems. Filamentous fungi belonging to the genera Fusarium, Monilia and Neurospora have been identified as potential organisms in recent years, that can convert cellulose directly to ethanol. Some species belonging to Fusarium, Mucor and Paecilomyces were also found to efficiently convert xylose to ethanol with high yields. Some fungal strains exhibited relatively higher ethanol and sugar tolerance. However, the major disadvantage with mycelial fungi for ethanol production is the slow bioconversion rate when compared to yeast. Potential bioethanol producing fungal strains, production of extracellular polysaccharases (cellulases and xylanases) and bioconversion of various carbohydrates are reviewed. The factors playing a significant role in determining culture variables and performance in lignocellulosic hydrolysate are discussed.
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Lipinski CV (1981) Science 212: 1466
Rosenberg SL (1980) Enz Microb Technol 2: 185
Klausmier WH (1983) Biotechnol Bioeng Symp 13: 81
Soltes EJ, Lin SCK (1983) Biotechnol Bioeng Symp 13: 53
Reed TB, Milne T, Diebold J, Derosier R (1981) Biotechnol Bioeng Symp 11: 137
Zeikus JG (1980) Ann Rev Microbiol 34: 423
Keim CR (1983) Enz Microb Technol 5: 103
Ladisch MR (1979) Proc Biochem 14: 21
Bullock JD (1979) Industrial alcohol. In: Bull AT, Ellwood, DC, Ratledge C (eds) Microbial Technology: Current Status and Future Prospects, Cambridge University Press, New York, p 309
Tsao GT (1978) Proc Biochem 13: 12
Stewart GG, Panchal CJ, Russel I, Sills AM (1984) CRC Crit Rev Biotechnol 1: 161
Schneider H, Wang PY, Chan YK, Maleszka R (1981) Biotechnol Lett 3: 89
Ueng PP, Gong CS (1982) Enz Microb Technol 4: 169
Jeffries TW (1984) Trends Biotechnol 3: 208
Wang DIC, Averingos GC, Biocic I, Wang SD, Fang HW (1983) Phil Trans Roy Soc London 300: 323
Jones RP, Pamment N, Greenfield PF (1981) Proc Biochem 16: 42
Hoffman H, Kuhlman W, Meyer H-D, Schügerl K. (1985) J Memb Sci 22: 235
Frick C, Schügerl K (1986) Appl Microbiol Biotechnol 25: 186
Zeikus JG, Ben-Bassat A, Ng TK, Lamed RJ (1981) Thermophilic ethanol fermentation. In: Hollaender A (ed) Trends in the Biology of Fermentation for Fuels and Chemicals, Plenum Press, New York, p 44
Rogers PL, Lee KJ, Tribe DE (1979) Biotechnol Lett 1: 165
Doelle HW (1982) Eur J Appl Microbiol Biotechnol 15: 20
Cromie S, Doelle HW (1981) Eur J Appl Microbiol Biotechnol 11: 16
Cromie S, Doelle HW (1982) Eur. J Appl Microbiol Biotechnol 14: 69
Dawes EA, Ribbons DW, Rees DA (1966) Biochem J 98: 804
Lee JH, Williamson D, Rogers PL (1981) Biotechnol Lett 3: 291
Karsh T, Stahl U, Esser K (1983) Eur J Appl Microbiol Biotechnol 18: 383
Rose AH, Harrison JS (1971) The Yeasts, Vol 2 & 3, Academic Press, New York
Lowitt RW, Kim BH, Shen G-J, Zeikus JG (1988) CRC Crit Rev Biotechnol 7: 107
Marwaha SS, Kennedy JF (1984) Enz Microb Technol 6: 18
Frelot D, Moulin G, Galzy P (1982) Biotechnol Lett 4: 411
Margarits A, Bajpai P, Cannel E (1981) Biotechnol Lett 3: 595
O'Leary VS, Sutton C, Bencivengo M, Sullivan B, Holsinger VA (1977) Biotechnol Bioeng 19: 1689
Enari T-M, Suihko M-L (1984) CRC Crit Rev Biotechnol 1: 229
Schneider H (1989) CRC Crit Rev Biotechnol 9: 1
Pelvako EA, Czeban ME (1935) Mikrobiologiya 4: 86
Wang PY, Shopsis C, Schneider H (1980) Biochem Biophys Res Comm 94: 248
Wang PY, Johnson BF, Schneider H (1980) Biotechnol Lett 3: 273
Silinger PJ, Botahst RJ, van Cauwenberge JE, Kurtzman CP (1982) Biotechnol Bioeng 24: 371
Sreenath HK, Chapman TW, Jeffries TW (1986) Appl Microbiol Biotechnol 24: 294
Schvester P, Robinson CW, Moo-Young M (1983) Biotechnol Bioeng Symp 13: 131
Gong CS, Chen LF, Flickinger MC, Chiang LC, Tsao GT (1981) Appl Environ Microbiol 41: 430
Karczewska H (1959) CR Trav Lab Carlsberg 31: 251
Schneider H (1982) The production of ethanol from d-xylose by yeasts. In: Proc. Roy Soc Can Int Symp Ethanol From Biomass, Winnipeg, p 508
Maleszka R, Veliky IA, Schneider H (1981) Biotechnol Lett 3: 415
Kosaric N, Ng DCM, Russel I, Stewart GG (1982) Adv Appl Microbiol 24: 147
Spangler DJ, Emert GH (1986) Biotechnol Bioeng 28: 115
Ooshima H, Ishtani Y, Harano Y (1985) Biotechnol Bioeng 27: 389
Spindler DD, Wyman, CE, Grohmann K (1989) Biotechnol Bioeng 34: 189
Deshpande V, Sivaraman H, Rao M (1983) Biotechnol Bioeng 25: 1679
Saddler JN, Hogan C, Chan MKH, Louis-Seize G (1982) Can J Microbiol 28: 1311
Ghosh P, Pamment NB, Martin WRB (1982) Enz Microb Technol 4: 425
Klyosov AA, Consultant V (1986) Appl Biochem Biotechnol 12: 249
Szczodrak J, Targonski Z (1988) Biotechnol Bioeng 31: 300
Chen S, Wayman M (1989) Proc Biochem 24: 204
Cooney CL, Wang DIC, Wang S-D, Gordon J, Jiminez M (1978) Biotechnol Bioeng Symp 8: 103
Florenzano G, Poulain M, Goma G (1984) Biomass 4: 295
Saddler JN, Chan MKH, Seize GL, (1981) Biotechnol Lett 3: 321
Ng TK, Bassat AB, Zeikus JG (1981) Appl Environ Microbiol 41: 1337
Hynn HH, Shen GJ, Zeikus JG (1985) J Bacteriol 164: 1153
Averinos GC, Fang HY, Biocic I, Wang DIC (1981) Adv Biotechnol 2: 119
Gong CS, Mann CM, Tsao GT (1981) Biotechnol Lett 3: 77
Rao M, Deshpande V, Keskar S, Srinivasan MC (1983) Enz Microb Technol 5: 133
Deshpande V, Keskar S, Mishra C, Rao M (1986) Enz Microb Technol 8: 149
Christakopoulos P, Macris BJ, Kekos D (1989) Enz Microb Technol 11: 236
Christakopoulos P, Macris BJ, Kekos D (1990) Appl Microbiol Biotechnol 33: 18
Kumar PKR, Singh A, Schügerl K (1991) Appl Microbiol Biotechnol 34: 570–572
Singh A (1988) Biochemical studies on utilization of agricultural wastes using cellulolytic fungi. Thesis, N.D. University of Agriculture and Technology, India
Marsden WL, Gray PP (1986) CRC Crit Rev Biotechnol 3: 235
Jeffries TW (1983) Adv Biochem Eng Biotechnol 27: 2
Rogers PL, Lee KJ, Tribe DE (1980) Adv Biochem Eng Biotechnol 23: 37
Swings J, De Ley I (1977) Bacteriol Rev 41: 1
Zeikus JG (1979) Enz Microb Technol 1: 243
Schneider H, Maleszka R, Neirinck L, Veliky IA, Wang PY, Chan YK (1983) Adv Biochem Eng/Biotechnol 25: 57
Skoog K, Hahn-Hägerdal B (1988) Enz Microb Technol 10: 66
Cochrane VW (1958) Physiology of Fungi, John Wiley and Sons Inc., London, p 214
Perlman D (1950) Am J Bot 37: 237
Suihko M-L, Enari T-M (1981) Biotechnol Lett 3: 723
Batter TR, Wilke CR (1977) A study of the fermentation of xylose to ethanol by Fusarium oxysporum Energy and environment division, Lawrence Berkley Laboratory, University of California, Berkley, 1977
Antonopoulos AA, Wene EG (1984) Phytopathol 74: 1268
Rosenberg SI, Batter TR, Blanch HW, Wilke CR (1981) AIChE Symp Ser 77, 107
Suihko M-L (1983) Biotechnol Lett 5: 721
Linko M, Viikari L, Suihko ML (1984) Biotechnol Adv 2: 233
Waksman SA, Foster JW (1938) J Agri Res 57: 873
Ward GE, Lockwood LB, Tabenkin B, Well PA (1938) Ind Eng Chem 30: 1233
Perlman D (1949) Am J Bot 36: 180
Wu JF, Lastick SM, Updegraff DM (1986) Nature 321, 887
Anderson AK (1924) Minn St Plant Sci 5: 237
Letcher H, Willmann JJ (1926) Phytopathol 16: 941
Antonopoulos AA (1979) Fusarium species: Their potential for transforming biomass to ethanol. Energy and Environmental Systems Division, Argonne National Laboratory, Argonne, Illinois 60439
Fiechter A, Furhmann GF, Käppeli O (1981) Adv Microb Physiol 35: 454
Chiang C, Knight SG (1959) Biochim Biophys Acta 35: 454
Chiang C, Knight SG (1960) Nature 118: 79
Suhiko M-L, Suomalainen I, Enari T-M (1983) Biotechnol Lett 5: 525
Tomoyeda M, Horitsn H (1964) Agric Biol Res 28: 139
Horitsu H (1973) Proc 3rd Int Spec Symp Yeasts, Helsinki, p 136
Hofer M, Betz A, Kotyk A (1971) Biochem Biophys Acta 252: 1
Zanek J, Kalecikova B, Kuniak L, Kucas S, Kockovakratochvilova A (1986) Acta Aliment 15: 111
Chiang C, Knight SG (1960) Biochem Biophys Res Comm 3: 554
Peixoto BG, Viega LA (1974) Arq Biol Technol 17: 87
Gibbs M, Cochrane VW, Paege LM, Wolin H (1951) Arch Biochem Biophys 33: 346
Coleman RJ, Nord FF (1951) Arch Biochem Biophys 33: 346
Halliwell G, (1961) Biochem J 79: 185
Halliwell G, Griffin M (1973) J Gen Microbiol 216: 211
Alexander M (1977) Introduction to Soil Microbiology, Wiley Eastern Ltd., New Delhi, p 386
Pathak AN, Ghosh TK (1973) Proc Biochem 8: 35
Mandels M, Weber J, Adv Biochem Ser 95: 391
Shewale JG, Sadana JC (1978) Can J Microbiol 24: 1204
Singh A, Abidi AB, Darmwal NS, Agrawal AK (1988) Bioconversion of agricultural lignocellulosic residues for the production of protein and cellulase in solid state culture. “Application of Biotechnology for Agriculture and Rural Development”, NIRD, Hyderabad, p 68
Tangnu SK, Blanch HW, Wilke CR (1981) Biotechnol Bioeng 23: 1837
Ishaque M, Kluepfel D (1981) Biotechnol Lett 3: 481
Viikari L, Klemola M, Linko M (1978) Production of xylanases. Proc. Bioconversion in Food Technology, Helsinki, p 115
Mishra C, Keskars, Rao M (1984) Appl Environ Microbiol 48, 224
Lynd LR (1989) Adv Biochem Eng/Biotechnol 38: 1
Chiang LC, Hsiao HY, Flickinger MC, Chen LF, Tsao GT (1982) Enz Microb Technol 4: 93
Kumar PKR, Lonsane BK (1989) Adv Appl Microbiol 34: 29
Schneider H (1989) Conversion of hemicelluloses into ethanol. In: Chahal DS (ed) Food, Feed and Fuel from Biomass, Oxford and I.B.H. Publishing C, New Delhi
Silinger PJ, Bothast RJ, Okos MR, Ladisch MR (1985) Biotechnol Lett 7: 431
Jeffries, TW, Fady JH, Lightfoot, FN (1985) Biotechnol Lett 7: 171
du Preez JC, Bosch M, Prior BA (1986) Enz Microb Technol 8: 360
Sale A (1967) Regulation of carbohydrate transport and metabolism in yeast. In: Mills AK, Krebs H (Eds) Aspects of Yeast Metabolism, F. A. Davies & Co., Philadelphia, p 45
Chung IS, Lee YY (1986) Enz Microb Technol 8: 503
Mahmourides G, Lee H, Maki N, Schneider H (1985) Bio/Technology 3: 59
D'Amore T, Stewart GG (1987) Enz Microb Technol 9: 322
Takagawa T, Wmezu M (1979) J Gen Appl Microbiol 25: 41
Lee YY and McCaskey TA (1983) Tappi J 66: 102
Hueting S, Tempest DW (1977) Arch Microbiol 115: 73
Singh, A, Kumar, PKR, Schügerl, K (1991) in preparation
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Singh, A., Kumar, P.K.R., Schügerl, K. (1992). Bioconversion of cellulosic materials to ethanol by filamentous fungi. In: Enzymes and Products from Bacteria Fungi and Plant Cells. Advances in Biochemical Engineering/Biotechnology, vol 45. Springer, Berlin, Heidelberg. https://doi.org/10.1007/BFb0008755
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DOI: https://doi.org/10.1007/BFb0008755
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