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Genome-wide analysis of plant metal transporters, with an emphasis on poplar

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Abstract

The specific transport of metal ions, mediated by membrane-localized metal transporters, is of fundamental importance in all eukaryotes. Genome-wide analysis of metal transporters was undertaken, making use of whole genome sequences of the green alga Chlamydomonas reinhardtii, the moss Physcomitrella patens, the lycophyte Selaginella moellendorffii, the monocots rice and sorghum, and the dicots Arabidopsis thaliana, poplar, grapevine, as well as of the yeast Saccharomyces cerevisiae. A repertoire of 430 metal transporters was found in total across eight photosynthetic plants, as well as in S. cerevisiae. Seventy-two full-length metal transporter genes were identified in the Populus genome alone, which is the largest number of metal transporters genes identified in any single species to date. Diversification of some transporter family gene clusters appears to have occurred in a lineage-specific manner. Expression analysis of Populus metal transporters indicates that some family members show tissue-specific transcript abundance. Taken together, the data provide a picture into the diversification of these important gene families.

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Acknowledgments

This work was supported by an ANR PRECODD project (ANR-06-ECOT-O15-01) and by a PhD grant to A. Migeon from the Agence de l’Environnement et de la Maîtrise de l’Energie (ADEME) and the Conseil Régional de Lorraine. OW was generously supported by a Natural Science and Engineering Research Council of Canada (NSERC) Canadian Graduate Scholarship (CGSD). This work was generously supported in part by funding from NSERC to MMC.

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Correspondence to Michel Chalot.

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18_2010_445_MOESM3_ESM.ppt

Figure S1. An unrooted, neighbor-joining (NJ)-based tree of the zinc–iron permeases (ZIP) family in selected plants. The analysis was performed as described in the “Materials and methods” section and the tree was generated using MEGA version 4.1 [28] after sequence alignment. Distances were calculated with the Poisson correction model for distance computation (homogeneous substitution pattern among lineages). Bootstrap values are indicated (1,000 replicates). Branch lengths are proportional to phylogenetic distances. Corresponding gene loci are given in a supplemental file. Names of the species are given in the legend of Fig. 1. Supplementary material 3 (PPT 559 kb)

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Migeon, A., Blaudez, D., Wilkins, O. et al. Genome-wide analysis of plant metal transporters, with an emphasis on poplar. Cell. Mol. Life Sci. 67, 3763–3784 (2010). https://doi.org/10.1007/s00018-010-0445-0

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  • DOI: https://doi.org/10.1007/s00018-010-0445-0

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