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Allele-specific differential expression of a common adiponectin gene polymorphism related to obesity

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Abstract

Adiponectin gene polymorphisms have recently been reported to be associated with obesity, insulin sensitivity, and the risk of type 2 diabetes. We examined a T94G polymorphism of the adiponectin gene in 245 ostensibly normal nondiabetic subjects. The G allele frequency was lower among subjects with higher BMI (≥27) than in those with lower BMI. BMI was inversely correlated with the dose of G allele. Multivariate linear regression analyses showed that the adiponectin genotypes were significantly related to BMI after adjusting for age and gender. The dose of the G allele was associated with a reduction of approximately 1.12 kg/m2 in BMI. We further found that the relative mRNA levels of G allele were consistently higher than those of T allele in the omental adipose tissue from 21 heterozygous subjects. Finally, we observed that the expression levels of adiponectin affected insulin-stimulated glucose uptake in differentiated 3T3-L1 adipocytes. In conclusion, the allele-specific differential expression of this common polymorphism could be responsible for its biological effects observed in this and the other studies.

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Abbreviations

BMI :

Body mass index

SNP :

Single nucleotide polymorphism

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Acknowledgements

This work was supported by the Program for Promoting Academic excellence of Universities (89-B-FA01-1-4) from the Ministry of Education and a grant from the National Research Program for Genomic Medicine, National Science Council (NSC 91-3112-B-002-019) of the Republic of China.

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Authors and Affiliations

  1. Division of Endocrinology and Metabolism, Departments of Internal Medicine, National Taiwan University Hospital, 7 Chung-Shan South Road, Taipei, Taiwan

    Wei-Shiung Yang, Chi-Chung Peng, Kuan-Ching Lee, Mei-Ju Chen, Tong-Yuan Tai & Lee-Ming Chuang

  2. Department of Clinical Pathology, National Taiwan University Hospital, 7 Chung-Shan South Road, Taipei, Taiwan

    Pei-Ling Tsou

  3. Graduate Institute of Clinical Medicine, National Taiwan University Medical College, Taipei, Taiwan

    Wei-Shiung Yang, Da-Lun Tseng & Lee-Ming Chuang

  4. Institute of Epidemiology, National Taiwan University, Taipei, Taiwan

    Chi-Ling Chen

  5. Department of Surgery, En-Chu-Kong Hospital, Taipei Hsien, Taiwan

    Wei-Jei Lee

  6. Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan

    Chang-Jen Huang

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  1. Wei-Shiung Yang
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Correspondence to Lee-Ming Chuang.

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Yang, WS., Tsou, PL., Lee, WJ. et al. Allele-specific differential expression of a common adiponectin gene polymorphism related to obesity. J Mol Med 81, 428–434 (2003). https://doi.org/10.1007/s00109-002-0409-4

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  • DOI: https://doi.org/10.1007/s00109-002-0409-4

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