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Genetic linkage maps of two apricot cultivars (Prunus armeniaca L.) compared with the almond Texas × peach Earlygold reference map for Prunus

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Abstract

Several genetic linkage maps have been published in recent years on different Prunus species suggesting a high level of resemblance among the genomes of these species. One of these maps (Joobeur et al., Theor Appl Genet 97:1034–1041 [(1998); Aranzana et al., Theor Appl Genet 106:819–825 (2002b)] constructed from interspecific almond Texas × peach Earlygold F2 progeny (T×E) was considered to be saturated. We selected 142 F1 apricot hybrids obtained from a cross between P. armeniaca cvs. Polonais and Stark Early Orange for mapping. Eighty-eight RFLP probes and 20 peach SSR primer pairs used for the ‘reference map’ were selected to cover the eight linkage groups. One P. davidiana and an additional 14 apricot simple sequence repeats (SSRs) were mapped for the F1 progeny. Eighty-three amplified fragment length polymorphisms were added in order to increase the density of the maps. Separate maps were made for each parent according to the ‘double pseudo-testcross’ model of analysis. A total of 141 markers were placed on the map of Stark Early Orange, defining a total length of 699 cM, and 110 markers were placed on the map of Polonais, defining a total length of 538 cM. Twenty-one SSRs and 18 restriction placed in the T×E map were heterozygous in both parents (anchor loci), thereby enabling the alignment of the eight homologous linkage groups of each map. Except for 15 markers, most markers present in each linkage group in apricot were aligned with those in T×E map, indicating a high degree of colinearity between the apricot genome and the peach and almond genomes. These results suggest a strong homology of the genomes between these species and probably between Prunophora and Amygdalus sub-genera.

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Acknowledgements

The authors wish to thank Jocelyne Kervella and Mireille Faurobert (GAFL-INRA Avignon) for their critical reading of this manuscript and all of the groups involved in the European Prunus mapping project (AIR3-CT93-1585) for kindly providing the DNA probes and SSR primers that we used for mapping—Stephane and Véronique Decroocq (UREFV-INRA-Bordeaux) for providing the apricot cDNA-SSR clones; Isabelle Marty and Gaia Sarkissian (SQPOV-INRA-Avignon) for the apricot probes; Jamila Chaib, Guillaume Gerez and Lamia Krichen for their participation. They also wish to thank Guy Clauzel, INRA-Domaine de Gotheron for maintaining the hybrid population. This work and that of L.S. Hagen were funded by the European project ABRIGEN-FAIR6 CT98–4345 “Optimization of apricot improvement by a joint conventional and molecular approach applied to the main agronomic traits”.

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Authors and Affiliations

  1. Unité Génétique et Amélioration des Fruits et Légumes, INRA, Domaine Saint Maurice, 84143, Montfavet Cedex, France

    P. Lambert, L. S. Hagen & J. M. Audergon

  2. Departament de Genètica Vegetal, IRTA, Carretera de Cabrils s/n, 08348, Cabrils-Barcelona, Spain

    P. Arus

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  1. P. Lambert
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  2. L. S. Hagen
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  3. P. Arus
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  4. J. M. Audergon
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Correspondence to J. M. Audergon.

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Communicated by C. Möllers

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Lambert, P., Hagen, L.S., Arus, P. et al. Genetic linkage maps of two apricot cultivars (Prunus armeniaca L.) compared with the almond Texas × peach Earlygold reference map for Prunus . Theor Appl Genet 108, 1120–1130 (2004). https://doi.org/10.1007/s00122-003-1526-3

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