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Quantitative trait loci for broomrape (Orobanche cumana Wallr.) resistance in sunflower

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Abstract

Broomrape (Orobanche cumana Wallr.) is a root parasite of sunflower that is regarded as one of the most important constraints of sunflower production in the Mediterranean region. Breeding for resistance is the most effective method of control. P-96 is a sunflower line which shows dominant resistance to broomrape race E and recessive resistance to the very new race F. The objective of this study was to map and characterize quantitative trait loci (QTL) for resistance to race E and to race F of broomrape in P-96. A population from a cross between P-96 and the susceptible line P-21 was phenotyped for broomrape resistance in four experiments, two for race E and two for race F, by measuring different resistance parameters (resistance or susceptibility, number of broomrape per plant, and proportion of resistant plants per F3 family). This population was also genotyped with microsatellite and RFLP markers. A linkage map comprising 103 marker loci distributed on 17 linkage groups was developed, and composite interval mapping analyses were performed. In total, five QTL (or1.1, or3.1, or7.1 or13.1 and or13.2) for resistance to race E and six QTL (or1.1, or4.1, or5.1, or13.1, or13.2 and or16.1) for resistance to race F of broomrape were detected on 7 of the 17 linkage groups. Phenotypic variance for race E resistance was mainly explained by the major QTL or3.1 associated to the resistance or susceptibility character (R 2=59%), while race F resistance was explained by QTL with a small to moderate effect (R 2 from 15.0% to 38.7%), mainly associated with the number of broomrape per plant. Or3.1 was race E-specific, while or1.1, or13.1 and or13.2 of were non-race specific. Or13.1, and or13.2 were stable across the four experiments. Or3.1, and or7.1 were stable over the two race E experiments and or1.1 and or5.1 over the two race F experiments. The results from this study suggest that resistance to broomrape in sunflower is controlled by a combination of qualitative, race-specific resistance affecting the presence or absence of broomrape and a quantitative non-race specific resistance affecting their number.

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Acknowledgements

B.P.V. was supported first by a post-doctoral grant from the Spanish Government (MEC, Ref. PF 98 28731590), and thereafter by a post-doctoral contract from the ‘Ramón y Cajal’ program (MCYT).

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Author notes

  1. S. T. Berry

    Present address: Advanta Seeds UK Ltd., Station Road, Docking, King’s Lynn, Norfolk, PE31 8LS, UK

Authors and Affiliations

  1. Instituto de Agricultura Sostenible (CSIC), Apartado 4084, 14080 , Córdoba, Spain

    B. Pérez-Vich, B. Akhtouch, L. Velasco & J. M. Fernández-Martínez

  2. Department of Crop and Soil Science, Oregon State University, Corvallis, OR 97331, USA

    S. J. Knapp

  3. Advanta Semillas, Ruta 226, Km 60, 7620 , Balcarce, Buenos Aires, Argentina

    A. J. Leon

  4. Advanta Biotechnology Laboratory, SES-Europe, 3300 , Tienen, Belgium

    S. T. Berry

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  1. B. Pérez-Vich
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Pérez-Vich, B., Akhtouch, B., Knapp, S.J. et al. Quantitative trait loci for broomrape (Orobanche cumana Wallr.) resistance in sunflower. Theor Appl Genet 109, 92–102 (2004). https://doi.org/10.1007/s00122-004-1599-7

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