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Microsatellite genetic linkage maps of myrobalan plum and an almond-peach hybrid—location of root-knot nematode resistance genes

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Abstract

Inheritance and linkage studies were carried out with microsatellite [or simple sequence repeat (SSR)] markers in a F1 progeny including 101 individuals of a cross between Myrobalan plum (Prunus cerasifera Ehrh) clone P.2175 and the almond (Prunus dulcis Mill.)-peach (Prunus persica L. Batsch) hybrid clone GN22 [‘Garfi’ (G) almond × ‘Nemared’ (N) peach]. This three-way interspecific Prunus progeny was produced in order to associate high root-knot nematode (RKN) resistances from Myrobalan and peach with other favorable traits for Prunus rootstocks from plum, peach and almond. The RKN resistance genes, Ma from the Myrobalan plum clone P.2175 and R MiaNem from the ‘N’ peach, are each heterozygous in the parents P.2175 and GN22, respectively. Two hundred and seventy seven Prunus SSRs were tested for their polymorphism. One genetic map was constructed for each parent according to the ‘double pseudo-testcross’ analysis model. The Ma gene and 93 markers [two sequence characterized amplified regions (SCARs), 91 SSRs] were placed on the P.2175 Myrobalan map covering 524.8 cM. The R MiaNem gene, the Gr gene controlling the color of peach leaves, and 166 markers (one SCAR, 165 SSRs) were mapped to seven linkage groups instead of the expected eight in Prunus. Markers belonging to groups 6 and 8 in previous maps formed a single group in the GN22 map. A reciprocal translocation, already reported in a G × N F2, was detected near the Gr gene. By separating markers from linkage groups 6 and 8 from the GN22 map, it was possible to compare the eight homologous linkage groups between the two maps using the 68 SSR markers heterozygous in both parents (anchor loci). All but one of these 68 anchor markers are in the same order in the Myrobalan plum map and in the almond-peach map, as expected from the high level of synteny within Prunus. The Ma and R MiaNem genes confirmed their previous location in the Myrobalan linkage group 7 and in the GN22 linkage group 2, respectively. Using a GN22 F2 progeny of 78 individuals, a microsatellite map of linkage group 2 was also constructed and provided additional evidence for the telomeric position of R MiaNem in group 2 of the Prunus genome.

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Acknowledgements

This work was partly funded by the Commission of the European Union via the FAIR Program of Research and Technological Development (Research project No. FAIR6-CT 98-4139; 1999–2003) and by the Conseil Regional d’Aquitaine (2000–2002). The authors thank the technical staff of the INRA ‘Domaine des Jarres’ experimental farm (Unité Expérimentale Arboricole) for cultivating the interspecific Myrobalan plum × almond-peach hybrids. The authors also wish to thank Patrick Lambert (INRA, Avignon, France) for the AMPA SSR primers.

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Authors and Affiliations

  1. INRA, Unité de Recherches sur les Espèces Fruitières et la Vigne (UREFV), B.P. 81, 33883, Villenave d’Ornon Cedex, France

    E. Dirlewanger, P. Cosson, G. Capdeville, C. Poizat, B. Lafargue, O. Baron, F. Laigret & M. Kleinhentz

  2. INRA, UMR “Interactions Plantes-Microorganismes et Santé Végétale” (IPMSV), Equipe de Nématologie, 400, route des Chappes, B.P. 167, 06903, Sophia Antipolis Cedex, France

    N. Bosselut, M. Claverie, R. Voisin & D. Esmenjaud

  3. IRTA, Departement de Genetica Vegetal, Carretera de Cabrils s/n, 08348, Cabrils, Spain

    W. Howad & P. Arús

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  1. E. Dirlewanger
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  2. P. Cosson
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  4. G. Capdeville
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Correspondence to E. Dirlewanger.

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Dirlewanger, E., Cosson, P., Howad, W. et al. Microsatellite genetic linkage maps of myrobalan plum and an almond-peach hybrid—location of root-knot nematode resistance genes. Theor Appl Genet 109, 827–838 (2004). https://doi.org/10.1007/s00122-004-1694-9

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