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STS markers linked to the Rf1 fertility restorer gene of cotton

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Abstract

Marker-assisted selection (MAS) can accelerate the process of plant breeding, and sequence-tagged site (STS) markers are highly specific for regions of DNA being used for MAS. The objective of this research was to develop STS markers tightly linked with Rf1, the fertility restoring gene for cytoplasmic male sterility (CMS) in cotton (Gossypium hirsutum L.). Bulked segregant analysis was employed to screen for Rf1-linked RAPD markers in a backcross population. Four RAPD markers were identified, three of which co-segregated with Rf1 (UBC1471400, UBC607500, and UBC679700). Another fragment, UBC169800, co-segregated with the previously reported UBC169700 in repulsion phase at a distance of 4.5 cM from Rf1. A marker published by others (UBC6591500) mapped to 2.7 cM from Rf1 and 1.8 cM from UBC169800. Four sets of STS primer pairs were designed based on the RAPD fragment sequences. The primer pairs from the UBC1471400 and UBC607500 fragments both amplified a single fragment specific to fertile plants. The UBC679700 and UBC6591500 STS primer pairs each amplified one fragment specific to fertile plants and a monomorphic fragment. These four Rf1-linked STS markers were also present in the Rf1 donor species G. harknessii (D2-2). The three primer pairs that produced co-segregating STS markers also amplified fragments from G. trilobum (D8). However, the D8 fragment amplified by the UBC1471400 STS primers was larger than that from D2-2, and G. trilobum does not restore fertility to CMS-D2-2 lines. These STS markers will be useful in the development of restorer parental lines in cotton CMS breeding efforts.

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Acknowledgements

This research was supported in part by a grant from Monsanto Company, St. Louis, Mo., USA.

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Authors and Affiliations

  1. Department of Crop, Soil, and Environmental Sciences, University of Arkansas, PTSC 115, Fayetteville, AR, 72701, USA

    C.-D. Feng & J. Mc D. Stewart

  2. Department of Agronomy and Horticulture, New Mexico State University, Skeen Hall, Room 127, Las Cruces, NM, USA

    J.-F. Zhang

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  1. C.-D. Feng
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  2. J. Mc D. Stewart
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  3. J.-F. Zhang
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Correspondence to J. Mc D. Stewart.

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Communicated by R. Bernardo

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Feng, CD., Stewart, J.M.D. & Zhang, JF. STS markers linked to the Rf1 fertility restorer gene of cotton. Theor Appl Genet 110, 237–243 (2005). https://doi.org/10.1007/s00122-004-1817-3

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