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Comparative analysis of the D genome-encoded high-molecular weight subunits of glutenin

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Abstract

Four genes encoding novel 1Dx-type high-molecular weight (HMW) subunits were amplified by polymerase chain reaction, two each from Aegilops tauschii and bread wheat Triticum aestivum. The two subunits from Ae. tauschii (1Dx2.1t and 1Dx2t) were both very similar in sequence to subunit 1Dx2 from bread wheat. In contrast, the two novel bread wheat subunits (1Dx2.2 and 1Dx2.2*) differed from subunit 1Dx2 in having different internally duplicated regions (of 132 and 186 amino acid, respectively) within their repetitive domains. These duplicated sequences were located adjacent to the regions from which they had been duplicated and had complete intact repeat motifs at each end. The implications of these results for HMW subunit evolution and wheat quality improvement are discussed.

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Acknowledgements

Rothamsted Research receives grant-aided support from the Biotechnology and Biological Sciences Research Council of the United Kingdom. Yongfang Wan was supported by a Royal Society China-UK Joint Project Grant (Q813 “Aegilops species as a source of novel genes for wheat improvement”). Daowen Wang was supported by grants (2002CB111301, 2003AA222040) from the Ministry of Science and Technology of China.

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Authors and Affiliations

  1. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China

    Yongfang Wan, Zehong Yan, Kunfan Liu & Daowen Wang

  2. Rothamsted Research, Harpenden, Hertfordshire, AL5 2JQ, UK

    Yongfang Wan, Peter R. Shewry & Nigel G. Halford

  3. University of Tuscia, Via SC De Lellis, 01100, Viterbo, Italy

    Renato D’Ovidio

  4. Triticeae Research Institute, Sichuan Agricultural University, Dujiangyan, 611830, China

    Zehong Yan & Youliang Zheng

Authors
  1. Yongfang Wan
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  2. Zehong Yan
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  3. Kunfan Liu
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  4. Youliang Zheng
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  5. Renato D’Ovidio
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  6. Peter R. Shewry
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  7. Nigel G. Halford
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  8. Daowen Wang
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Corresponding author

Correspondence to Peter R. Shewry.

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Communicated by P. Langridge

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Wan, Y., Yan, Z., Liu, K. et al. Comparative analysis of the D genome-encoded high-molecular weight subunits of glutenin. Theor Appl Genet 111, 1183–1190 (2005). https://doi.org/10.1007/s00122-005-0051-y

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  • DOI: https://doi.org/10.1007/s00122-005-0051-y

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