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A composite linkage map from two crosses for the species complex Picea mariana × Picea rubens and analysis of synteny with other Pinaceae

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Abstract

Four individual linkage maps were constructed from two crosses for the species complex Picea mariana (Mill.) B.S.P. × Picea rubens Sarg in order to integrate their information into a composite map and to compare with other Pinaceae. For all individual linkage maps, 12 major linkage groups were recovered with 306 markers per map on average. Before building the composite linkage map, the common male parent between the two crosses made it possible to construct a reference linkage map to validate the relative position of homologous markers. The final composite map had a length of 2,319 cM (Haldane) and contained a total of 1,124 positioned markers, including 1,014 AFLPs, 3 RAPDs, 53 SSRs, and 54 ESTPs, assembled into 12 major linkage groups. Marker density of the composite map was statistically homogenous and was much higher (one marker every 2.1 cM) than that of the individual linkage maps (one marker every 5.7 to 7.1 cM). Synteny was well conserved between individual, reference, and composite linkage maps and 94% of homologous markers were colinear between the reference and composite maps. The combined information from the two crosses increased by about 24% the number of anchor markers compared to the information from any single cross. With a total number of 107 anchor markers (SSRs and ESTPs), the composite linkage map is a useful starting point for large-scale genome comparisons at the intergeneric level in the Pinaceae. Comparisons of this map with those in Pinus and Pseudotsuga allowed the identification of one breakdown in synteny where one linkage group homoeologous to both Picea and Pinus corresponded to two linkage groups in Pseudotsuga. Implications for the evolution of the Pinaceae genome are discussed.

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Acknowledgements

We thank F. Gagnon, I. Gosselin, M. Lamothe, S. Plante, and S. Senneville (Univ. Laval and Canadian Forest Service) for their help and support in the laboratory, and with mapping and sequence analyses. We also thank R.K. Rutledge (Canadian Forest Service) for the gift of HAP3a and HAP3b from P. mariana. Finally, we thank P. Meirmans (Canadian Forest Service), O. Savolainen (University of Oulu, Finland) and two anonymous reviewers for their constructive comments on previous drafts of this manuscript. Pedigree material was developed by N.I. and J. Beaulieu (Canadian Forest Service) through a Canadian Biotechnology Strategy grant. This research was supported by a National Sciences and Engineering Research Council of Canada grant (Genomics Program) to J.B. and N.I.

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  1. Chaire de recherche du Canada en génomique forestière et environnementale, Centre de recherche en biologie forestière, Pavillon Charles-Eugène-Marchand, Université Laval, Sainte-Foy, QC, G1K 7P4, Canada

    Betty Pelgas, Jean Bousquet, Stéphanie Beauseigle & Nathalie Isabel

  2. Natural Resources Canada, Laurentian Forestry Centre, Canadian Forest Service, 1055 Rue du PEPS, C.P. 3800, Sainte-Foy, QC, G1V 4C7, Canada

    Nathalie Isabel

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  4. Nathalie Isabel
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Correspondence to Nathalie Isabel.

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Communicated by O. Savolainen

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Pelgas, B., Bousquet, J., Beauseigle, S. et al. A composite linkage map from two crosses for the species complex Picea mariana × Picea rubens and analysis of synteny with other Pinaceae. Theor Appl Genet 111, 1466–1488 (2005). https://doi.org/10.1007/s00122-005-0068-2

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  • DOI: https://doi.org/10.1007/s00122-005-0068-2

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