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BAC libraries of Triticum urartu, Aegilops speltoides and Ae. tauschii, the diploid ancestors of polyploid wheat

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Abstract

Triticum urartu, Aegilops speltoides and Ae. tauschii are respectively the immediate diploid sources, or their closest relatives, of the A, B and D genomes of polyploid wheats. Here we report the construction and characterization of arrayed large-insert libraries in a bacterial artificial chromosome (BAC) vector, one for each of these diploid species. The libraries are equivalent to 3.7, 5.4 and 4.1 of the T. urartu, Ae. speltoides, Ae. tauschii genomes, respectively. The predicted levels of genome coverage were confirmed by library hybridization with single-copy genes. The libraries were used to estimate the proportion of known repeated nucleotide sequences and gene content in each genome by BAC-end sequencing. Repeated sequence families previously detected in Triticeae accounted for 57, 61 and 57% of the T. urartu, Ae. speltoides and Ae. tauschii genomes, and coding regions accounted for 5.8, 4.5 and 4.8%, respectively.

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Acknowledgements

We thank USDA/CSREES/NRICGP for financial support by grant 2001-35301-10594.

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  1. Department of Plant Sciences, University of California, Davis, CA, 95616, USA

    E. D. Akhunov, A. R. Akhunova & J. Dvořák

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  1. E. D. Akhunov
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  2. A. R. Akhunova
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  3. J. Dvořák
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Correspondence to J. Dvořák.

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Communicated by P. Langridge

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Akhunov, E.D., Akhunova, A.R. & Dvořák, J. BAC libraries of Triticum urartu, Aegilops speltoides and Ae. tauschii, the diploid ancestors of polyploid wheat. Theor Appl Genet 111, 1617–1622 (2005). https://doi.org/10.1007/s00122-005-0093-1

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