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A new aluminum tolerance gene located on rye chromosome arm 7RS

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Abstract

Rye has one of the most efficient groups of genes for aluminum tolerance (Alt) among cultivated species of Triticeae. This tolerance is controlled by, at least, three independent and dominant loci (Alt1, Alt2, and Alt3) located on chromosome arms 6RS, 3RS, and 4RL, respectively. The segregation of Alt genes and several random amplified polymorphic DNA (RAPD), Secale cereale inter-microsatellite (SCIM), and Secale cereale microsatellite (SCM) markers in three F2 between a tolerant cultivar (Ailés) and a non-tolerant inbred line (Riodeva) were studied. The segregation ratio obtained for aluminum tolerance in the three F2 populations analyzed was 3:1 (tolerant:non-tolerant), indicating that tolerance is controlled by one dominant locus. SCIM811 1376 was linked to an Alt gene in the three F2 populations studied, and three different SCIMs and one RAPD (SCIM811 1376 , SCIM812 626 , SCIM812 1138 , and OPQ4 725 ) were linked to the Alt gene in two F2 populations. This result indicated that the same Alt gene was segregating in the three crosses. SCIM819 1434 and OPQ4 578 linked to the tolerance gene in one F2 population were located using wheat–rye ditelosomic addition lines on the 7RS chromosome arm. The Alt locus is mapped between SCIM819 1434 and the OPQ4 578 markers. Two microsatellite loci (SCM-40 and SCM-86), previously located on chromosome 7R, were also linked to the Alt gene. Therefore, the Alt gene segregating in these F2 populations is new and probably could be orthologous to the Alt genes located on wheat chromosome arm 4DL, on barley chromosome arm 4HL, on rye chromosome arm 4RL, and rice chromosome 3. This new Alt gene located on rye chromosome arm 7RS was named Alt4. A map of rye chromosome 7R with the Alt4 gene, 16 SCIM and RAPD, markers and two SCM markers was obtained.

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Acknowledgements

This work was supported by the research grants BOS 2000-0561 and AGL 200306470 from the Ministry of Education and Science of Spain (DGICYT) and Praxis XXII/15874/98 from the Ministry of Science and Technology (MCT) of Portugal.

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Authors and Affiliations

  1. Departamento de Genética, Facultad de Biología, Universidad Complutense, 28040, Madrid, Spain

    M. Matos, M. V. Camacho, V. Pérez-Flores, B. Pernaute & C. Benito

  2. Centro de Genética e Biotecnologia (CGB-UTAD), Universidade de Trás-os-Montes e Alto Douro, Apartado 1013, 5000-911, Vila Real, Portugal

    M. Matos & O. Pinto-Carnide

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  1. M. Matos
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Correspondence to C. Benito.

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Communicated by C. Möllers

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Matos, M., Camacho, M.V., Pérez-Flores, V. et al. A new aluminum tolerance gene located on rye chromosome arm 7RS. Theor Appl Genet 111, 360–369 (2005). https://doi.org/10.1007/s00122-005-2029-1

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