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Molecular analysis of Arachis interspecific hybrids

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Abstract

Incorporation of genetic resistance against several biotic stresses that plague cultivated peanut, Arachis hypogaea (2n=4x=40), is an ideal option to develop disease resistant and ecologically safe peanut varieties. The primary gene pool of peanut contains many diploid wild species (2n=2x=20) of Arachis, which have high levels of disease and insect resistances. However, transfer of resistant genes from these species into A. hypogaea is difficult due to ploidy level differences and genomic incompatibilities. This study was conducted to monitor alien germplasm transmission, using Random Amplified Polymorphic DNA (RAPD) markers, from two diploid wild species, A. cardenasii and A. batizocoi, into A. hypogaea. Triploid interspecific hybrids were produced by crossing two A. hypogaea cultivars (NC 6 and Argentine) with the two species and by colchicine-treating vegetative meristems, fertility was restored at the hexaploid (Co) level in the four hybrids. Hexaploids were allowed to self-pollinate for four generations, each referred to as a cycle (C1, C2, C3, and C4). At each cycle, a backcross was made with the respective A. hypogaea cultivar as the maternal parent and only lineages tracing back to a single hexaploid hybrid were used for RAPD analysis. Analysis of mapped, species-specific RAPD markers in BC1F1 to BC1F3 hybrids indicated that alien germplasm retention decreased every generation of inbreeding, especially in Argentine and in A. batizocoi crosses. A similar trend was also observed for every cycle in BC1F2 and BC1F3 families, possibly, due to the loss of alien chromosomes following selfing of hexaploids. RAPD marker analysis of 40–chromosome interspecific hybrid derivatives from the four crosses supported previous reports that reciprocal recombination and/or translocations are the predominant mechanisms for exchange of chromosomal segments. No evidence was found for preferential transfer of alien chromosomal regions to specific linkage groups. The implications for developing disease resistant peanut breeding lines are discussed in light of these findings.

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References

  • Abdou YAM, Gregory WC, Cooper WE (1974) Sources and nature of resistance to Cercospora arachidicola Hori and Cercosporidium personatum Berk. and Curt. Deighton in Arachis species. Peanut Sci 1:6–11

    Google Scholar 

  • Bianchi-Hall C, Keys RD, Stalker HT, Murphy JP (1993) Diversity of seed storage proteins in wild peanut (Arachis, Fabaceae) species. Plant Syst Evol 186:1–15

    Article  CAS  Google Scholar 

  • Company M, Stalker HT, Wynne JC (1982) Cytology and leaf spot resistance in Arachis hypogaea × wild species hybrids. Euphytica 31:885–894

    Article  Google Scholar 

  • Dhesi JS, Stalker HT (1994) Enhancing techniques for studying mitotic peanut chromosomes. Peanut Sci 21:92–94

    Google Scholar 

  • Garcia GM (1995) Evaluating efficiency of germplasm introgression from Arachis species to A. hypogaea L. PhD dissertation, Department of Crop Science, North Carolina State University

  • Garcia GM, Stalker HT, Kochert G (1995) Introgression analysis of an interspecific hybrid population in peanuts (Arachis hypogaea L.) using RFLP and RAPD markers. Genome 38:166–176

    PubMed  CAS  Google Scholar 

  • Garcia GM, Stalker HT, Schroeder E, Lyerly, JH and Kochert, G (2005) A RAPD-based linkage map of peanut based on a backcross population between the two diploid species Arachis stenosperma and A. cardenasii. Peanut Sci. (accepted)

  • Guok HP, Wynne JC, Stalker HT (1986) Recurrent selection within a population from an interspecific peanut cross. Crop Sci 26:249–253

    Article  Google Scholar 

  • Halward TM, Stalker HT (1987) Incompatibility mechanisms in interspecific peanut hybrids. Crop Sci 27:456–460

    Article  Google Scholar 

  • Halward T, Stalker HT, LaRue EA, Kochert G (1991) Genetic variation detectable with molecular markers among unadapted germplasm resources of cultivated peanut and related wild species. Genome 34:1013–1020

    CAS  Google Scholar 

  • Holbrook CC, Stalker HT (2003) Peanut breeding and genetic resources. Plant Breed Rev 22:297–356

    Google Scholar 

  • Kochert G, Halward T, Branch WC, Simpson CE (1991) RFLP variability in peanut (Arachis hypogaea L.) cultivars and wild species. Theor Appl Genet 81:565–570

    Article  CAS  Google Scholar 

  • Krapovickas A, Gregory WC (1994) Taxonomy of the genus Arachis (Leguminosae). Bonplandia 8:1–186

    Google Scholar 

  • Milla SR, Isleib TG, Stalker HT (2005) Taxonomic relationships among Arachis sect. Arachis species as revealed by AFLP markers. Genome 48:1–11

    Article  PubMed  CAS  Google Scholar 

  • Nelson SC, Starr JL, Simpson CE (1990) Expression of resistance to Meloidogyne arenaria in Arachis batizocoi and A. cardenasii. J Nematol 22:423–425

    PubMed  CAS  Google Scholar 

  • Reddy LJ, Nigam SN, Moss JP, Singh AK, Subrahmanyam P, McDonald D, Reddy AGS (1996) Registration of ICGV 86699 peanut germplasm line with multiple disease and insect resistance. Crop Sci 36:821

    Article  Google Scholar 

  • Simpson CE (1991) Pathways for introgression of pest resistance into Arachis hypogaea L. Peanut Sci 18:22–26

    Google Scholar 

  • Simpson CE, Starr JL (2001) Registration of ‘COAN’ peanut. Crop Sci 41:918

    Article  Google Scholar 

  • Smartt J, Gregory WC (1967) Interspecific cross-compatibility between the cultivated peanut Arachis hypogaea L. and other members of the genus Arachis. Oléagineux 22:455–459

    Google Scholar 

  • Spielman IV, Burge AP, Moss JP (1979) Chromosome loss and meiotic behavior in interspecific hybrids in the genus Arachis L. and their implications in breeding for disease resistance. Z Pflanzenzuchtg 53:236–250

    Google Scholar 

  • Stalker HT (1984) Utilizing Arachis cardenasii as a source of Cercospora leafspot resistance for peanut improvement. Euphytica 33:529–538

    Article  Google Scholar 

  • Stalker HT (1992) Utilizing Arachis germplasm resources. In: Nigam SN (ed) Groundnut—a global perspective. In: Proceedings of an international workshop, Hyderabad, India, 25–29 November 1991. International Crops Research Institute for the Semi-Arid Tropics, Patancheru, pp 281–295

  • Stalker HT, Beute MK (1993) Registration of four leaf-spot resistant germplasm lines. Crop Sci 33:1117

    Article  Google Scholar 

  • Stalker HT, Campbell WV (1983) Resistance of wild species of peanut to an insect complex. Peanut Sci 10:30–33

    Article  Google Scholar 

  • Stalker HT, Dalmacio RD (1981) Chromosomes of Arachis species, section Arachis. J Hered 72:403–408

    Google Scholar 

  • Stalker HT, Lynch RL (2002) Registration of four insect-resistant peanut germplasm lines. Crop Sci 42:313–314

    Article  PubMed  Google Scholar 

  • Stalker HT, Moss JP (1987) Speciation, cytogenetics and utilization of Arachis species. Adv Agron 41:1–40

    Article  Google Scholar 

  • Stalker HT, Simpson CE (1995) Germplasm resources in Arachis. In Pattee HE, Stalker HT (eds) Advances in peanut science. American Peanut Research and Educational Society, Stillwater

  • Stalker HT, Wynne JC, Company M (1979) Variation in progenies of an Arachis hypogaea × diploid wild species hybrid. Euphytica 28:675–684

    Article  Google Scholar 

  • Stalker HT, Phillips TD, Murphy JP, Jones TM (1994) Variation of isozyme patterns among Arachis species. Theor Appl Genet 87:746–755

    Article  CAS  Google Scholar 

  • Stalker HT, Shew BB, Garcia GM, Beute MK, Barker KR, Holbrook CC, Noe JP, Kochert GA (1995) Meloidogyne arenaria resistance in advanced-generation Arachis hypogaea × A. cardenasii hybrids. Proc Amer Peanut Res Educ Soc 27:24

    Google Scholar 

  • Stalker HT, Beute MK, Shew BB, Barker KR (2002a) Registration of two root-knot nematode-resistant peanut germplasm lines. Crop Sci 42:312–313

    Article  Google Scholar 

  • Stalker HT, Beute MK, Shew BB, Isleib TG (2002b) Registration of five leafspot-resistant peanut germplasm lines. Crop Sci 42:314–316

    Article  Google Scholar 

  • Subrahmanyam P, Ghanekar AM, Knolt BL, Reddy DVR, McDonald D (1985) Resistance to groundnut diseases in wild Arachis species. In: Moss JP (ed) Proceedings of the International Workshop on Cytogenetics of Arachis, Hyderabad, 31 Oct–2 Nov 1983. International Crops Research Institute for the Semi-Arid Tropics, Patancheru, pp 49–55

  • Tallury SP, Milla SR, Copeland SC, Stalker HT (2001) Genome donors of Arachis hypogaea L. Am Peanut Res Educ Soc 33:60

    Google Scholar 

  • Tallury SP, Hilu KW, Milla SR, Friend SA, Alsaghir M, Stalker HT, Quandt D (2005a) Genomic affinities in Arachis section Arachis (Fabaceae): molecular and cytogenetic evidence. Theor Appl Genet 111:1229–1237

    Article  CAS  Google Scholar 

  • Tallury SP, Isleib TG, Stalker HT (2005b) Developing peanut cultivars with genetic resistance to early leafspot. In: Proceedings of the American Peanut Research and Educational Society, vol 37 (in print)

  • Valls JFM, Simpson CE (2005) New Species of Arachis from Brazil, Paraguay and Bolivia. Bonplandia 14:1–2 (in press)

    Google Scholar 

  • Valls JFM, Rao VR, Simpson CE, Krapovickas A (1985) Current status of collection and conservation of South American groundnut germplasm with emphasis on wild species of Arachis. In: Moss JP (ed) Proceedings of the International Workshop on Cytogenetics of Arachis, Hyderabad, 31 Oct–2 Nov 1983. International Crops Research Institute for the Semi-Arid Tropics, Patancheru, pp 15–33

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Acknowledgments

The research reported herein was a cooperative effort of the North Carolina Agric. Res. Serv., a USDA competitive grant (#92-37300-7516) from the National Research Initiative Plant Genome Program, and the Peanut CRSP, USAID grant no. DAN-4048-G-SS-2065-00 and the recommendations represent neither an official position nor policy of the NCARS, USDA, or USAID. We also wish to thank Dr. J.S. Dhesi and J.A. Burns for their help with the chromosome counts and S.C. Copeland for her technical help during the crossing programs, seed planting, leaf collection, and DNA extractions.

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Correspondence to S. P. Tallury.

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Communicated by M. Kearsey

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Garcia, G.M., Tallury, S.P., Stalker, H.T. et al. Molecular analysis of Arachis interspecific hybrids. Theor Appl Genet 112, 1342–1348 (2006). https://doi.org/10.1007/s00122-006-0236-z

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  • DOI: https://doi.org/10.1007/s00122-006-0236-z

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