Abstract
Significant progress has been made in the construction of genetic maps in the tetraploid cotton Gossypium hirsutum. However, six linkage groups (LGs) have still not been assigned to specific chromosomes, which is a hindrance for integrated genetic map construction. In the present research, specific bacterial artificial chromosome (BAC) clones constructed in G. hirsutum acc. TM-1 for these six LGs were identified by screening the BAC library using linkage group-specific simple-sequence repeats markers. These BAC clones were hybridized to ten translocation heterozygotes of G. hirsutum. L as BAC-fluorescence in situ hybridization probes, which allowed us to assign these six LGs A01, A02, A03, D02, D03, and D08 to chromosomes 13, 8, 11, 21, 24, and 19, respectively. Therefore, the 13 homeologous chromosome pairs have been established, and we have proposed a new chromosome nomenclature for tetraploid cotton.
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Acknowledgements
This program was supported financially in part by Program for Changjiang Scholars and Innovative Research Team in University, the Ministry of Education, National Science Foundation in China (30070483, 30270806) Visiting Scholar Foundation of Key Laboratory in University (ZW2002003), and China Postdoctoral Science Foundation (2005037736). We thank Dr. X E Wang and Ms B Zhou, NAU, China for technical assistance.
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Wang, K., Song, X., Han, Z. et al. Complete assignment of the chromosomes of Gossypium hirsutum L. by translocation and fluorescence in situ hybridization mapping. Theor Appl Genet 113, 73–80 (2006). https://doi.org/10.1007/s00122-006-0273-7
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DOI: https://doi.org/10.1007/s00122-006-0273-7