Abstract
We report the first gene-based linkage map of Lupinus angustifolius (narrow-leafed lupin) and its comparison to the partially sequenced genome of Medicago truncatula. The map comprises 382 loci in 20 major linkage groups, two triplets, three pairs and 11 unlinked loci and is 1,846 cM in length. The map was generated from the segregation of 163 RFLP markers, 135 gene-based PCR markers, 75 AFLP and 4 AFLP-derived SCAR markers in a mapping population of 93 recombinant inbred lines, derived from a cross between domesticated and wild-type parents. This enabled the mapping of five major genes controlling key domestication traits in L. angustifolius. Using marker sequence data, the L. angustifolius genetic map was compared to the partially completed M. truncatula genome sequence. We found evidence of conserved synteny in some regions of the genome despite the wide evolutionary distance between these legume species. We also found new evidence of widespread duplication within the L. angustifolius genome.
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Acknowledgements
The mapping population originated from research begun by WAC during his employment at the Department of Agriculture Western Australia. Subsequent development of RILs, phenotyping, mapping of markers and comparative mapping was supported by Grains Research and Development Council (GRDC) UWA372, the Australian Research Council (ARC) LP0454871, and the Polish State Committee for Scientifc Research. Salaries of MNN and WAC are supported by a grant from the Export Grains Centre Ltd. The development of Lupinus spp.-derived cross-species marker primers were supported by GRDC UMU105 and ARC DP0559547. We thank Bevan Buirchell for providing seed for the RIL population, Sarah Brien for early assistance in the project and Michael Francki, Craig Atkins and Penny Smith for providing the L. angustifolius cDNA library.
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Communicated by J. S. Heslop-Harrison
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Nelson, M.N., Phan, H.T.T., Ellwood, S.R. et al. The first gene-based map of Lupinus angustifolius L.-location of domestication genes and conserved synteny with Medicago truncatula . Theor Appl Genet 113, 225–238 (2006). https://doi.org/10.1007/s00122-006-0288-0
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DOI: https://doi.org/10.1007/s00122-006-0288-0