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Transferable bread wheat EST-SSRs can be useful for phylogenetic studies among the Triticeae species

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Abstract

The genetic similarity between 150 accessions, representing 14 diploidand polyploid species of the Triticeae tribe, was investigated following the UPGMA clustering method. Seventy-three common wheat EST-derived SSR markers (EST-SSRs) that were demonstrated to be transferable across several wheat-related species were used. When diploid species only are concerned, all the accessions bearing the same genome were clustered together without ambiguity while the separation between the different sub-species of tetraploid as well as hexaploid wheats was less clear. Dendrograms reconstructed based on data of 16 EST-SSRs mapped on the A genome confirmed that Triticum aestivum and Triticum durum had closer relationships with Triticum urartu than with Triticum monococcum and Triticum boeoticum, supporting the evidence that T. urartu is the A-genome ancestor of polyploid wheats. Similarly, another tree reconstructed based on data of ten EST-SSRs mapped on the B genome showed that Aegilops speltoides had the closest relationship with T. aestivum and T. durum, suggesting that it was the main contributor of the B genome of polyploid wheats. All these results were expected and demonstrate thus that EST-SSR markers are powerful enough for phylogenetic analysis among the Triticeae tribe.

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Acknowledgments

The authors gratefully thank G. Gay and A. Loussert for growing the plants and J. David for providing with T. turgidum sub-species. G. Boutet and C. Pont are also greatly acknowledged for their help in managing the robotics of the genotyping platform. This work was funded by the China Scholarship Council (CSC). All these experiments comply with the current laws of France.

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Authors and Affiliations

  1. UMR INRA-UBP Amélioration et Santé des Plantes, Domaine de Crouël, 234 Avenue du Brézet, 63100, Clermont-Ferrand, France

    L. Y. Zhang, C. Ravel, M. Bernard, F. Balfourier, P. Leroy, C. Feuillet & P. Sourdille

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  1. L. Y. Zhang
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  2. C. Ravel
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  3. M. Bernard
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  4. F. Balfourier
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  7. P. Sourdille
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Correspondence to M. Bernard.

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Communicated by M. Sorrells

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Zhang, L.Y., Ravel, C., Bernard, M. et al. Transferable bread wheat EST-SSRs can be useful for phylogenetic studies among the Triticeae species. Theor Appl Genet 113, 407–418 (2006). https://doi.org/10.1007/s00122-006-0304-4

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  • DOI: https://doi.org/10.1007/s00122-006-0304-4

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