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Characterization of the HMW glutenin subunits from Aegilops searsii and identification of a novel variant HMW glutenin subunit

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Abstract

High molecular weight (HMW) glutenin subunits are conserved seed storage proteins in wheat and related species. Here we describe a more detailed characterization of the HMW glutenin subunits from Aegilops searsii, which is diploid and contains the Ss genome related to the S genome of Aegilops speltoides and the A, B and D genomes of hexaploid wheat. SDS-PAGE experiments revealed two subunits (one x and one y) for each of the nine Ae. searsii accessions analyzed, indicating that the HMW glutenin subunit gene locus of Ae. searsii is similar to the Glu-1 locus found in wheat in containing both x and y genes. The primary structure of the four molecularly cloned subunits (from two Ae. searsii accessions) was highly similar to that of the previously reported x and y subunits. However, in one accession (IG49077), the last 159 residues of the x subunit (1Ssx49077), which contained the sequence element GHCPTSPQQ, were identical to those of the y subunit (1Ssy49077) from the same accession. Consequently, 1Ssx49077 contains an extra cysteine residue located at the C-terminal part of its repetitive domain, which is novel compared to the x-type subunits reported so far. Based on this and previous studies, the structure and expression of the Glu-1 locus in Ae. searsii is discussed. A hypothesis on the genetic mechanism generating the coding sequence for the novel 1Ssx49077 subunit is presented.

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Acknowledgments

We thank Professor Moshe Feldman for constructive comments on this work and manuscript preparation. This work is supported by grants from Ministry of Science and Technology of China (2002CB111301, 2003AA222040) and the Chinese Academy of Sciences (KSCX2-SW-304).

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Authors and Affiliations

  1. The State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China

    Xia Sun, Xin Liu, Weiqiang Qian, Shanting Hao, Aimin Zhang & Daowen Wang

  2. College of Agriculture, Northeast Agricultural University, Harbin, 150030, China

    Xia Sun

  3. Southwest University of Science and Technology, Mianyang, 621010, China

    Xia Sun & Shanglian Hu

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  1. Xia Sun
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  2. Shanglian Hu
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  3. Xin Liu
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Correspondence to Daowen Wang.

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Communicated by J. W. Snape

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Sun, X., Hu, S., Liu, X. et al. Characterization of the HMW glutenin subunits from Aegilops searsii and identification of a novel variant HMW glutenin subunit. Theor Appl Genet 113, 631–641 (2006). https://doi.org/10.1007/s00122-006-0327-x

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