Abstract
We describe the construction of a reference genetic linkage map for the Brassica A genome, which will form the backbone for anchoring sequence contigs for the Multinational Brassica rapa Genome Sequencing Project. Seventy-eight doubled haploid lines derived from anther culture of the F1 of a cross between two diverse Chinese cabbage (B. rapa ssp. pekinensis) inbred lines, ‘Chiifu-401-42’ (C) and ‘Kenshin-402-43’ (K) were used to construct the map. The map comprises a total of 556 markers, including 278 AFLP, 235 SSR, 25 RAPD and 18 ESTP, STS and CAPS markers. Ten linkage groups were identified and designated as R1–R10 through alignment and orientation using SSR markers in common with existing B. napus reference linkage maps. The total length of the linkage map was 1,182 cM with an average interval of 2.83 cM between adjacent loci. The length of linkage groups ranged from 81 to 161 cM for R04 and R06, respectively. The use of 235 SSR markers allowed us to align the A-genome chromosomes of B. napus with those of B. rapa ssp. pekinensis. The development of this map is vital to the integration of genome sequence and genetic information and will enable the international research community to share resources and data for the improvement of B. rapa and other cultivated Brassica species.
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Acknowledgements
This study was supported by grants in Korea from Bio-Green 21 Program, Rural Development Administration from Ministry of Agriculture and Forestry; and Korean Science and Engineering Foundation (R21-2004-000-10010-0). In the UK the work was funded by the Biotechnology and Biological Sciences Research Council. We wish to thank Neale Grant, Carol Evered, Volkan Cevik, Jason Pole, and James Lynn at the Warwick HRI in UK, K Suwabe, M Matsumoto and M Hirai of the Kyoto Prefectural University in Japan, and Hayley Mountford, Clare Hopkins of the Plant Biotechnology Centre in Australia, Akihito Wakatsuki, Sakata Seed Corporation in Japan, Thomas C. Osborn at Seminis Vegetable Seeds Inc. in USA, and Andrew Sharpe, Clarke Wayne at the AAFC Saskatoon Research Centre in Canada for the contribution of the BLAST information.
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Choi, S.R., Teakle, G.R., Plaha, P. et al. The reference genetic linkage map for the multinational Brassica rapa genome sequencing project. Theor Appl Genet 115, 777–792 (2007). https://doi.org/10.1007/s00122-007-0608-z
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DOI: https://doi.org/10.1007/s00122-007-0608-z