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Resistance to Erysiphe necator in the grapevine ‘Kishmish vatkana’ is controlled by a single locus through restriction of hyphal growth

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Abstract

Vitis vinifera ‘Kishmish vatkana’, a cultivated grapevine from Central Asia, does not produce visible symptoms in response to natural or artificial inoculation with the fungus Erysiphe necator Schwein., the casual agent of powdery mildew. ‘Kishmish vatkana’ allowed pathogen entry into epidermal cells at a rate comparable to that in the susceptible control Vitis vinifera ‘Nimrang’, but was able to limit subsequent hyphal proliferation. Density of conidiophores was significantly lower in ‘Kishmish vatkana’ (33.6 ± 8.7 conidiophores mm−2) than in ‘Nimrang’ (310.5 ± 24.0 conidiophores mm−2) by 120 h after inoculation. A progeny of 310 plants from a ‘Nimrang’ × ‘Kishmish vatkana’ cross were scored for the presence or absence of visible conidiophores throughout two successive seasons. Phenotypic segregation revealed the presence of a single dominant allele termed Resistance to Erysiphe necator 1 (REN1), which was heterozygous in ‘Kishmish vatkana’. A bulked segregant analysis was carried out using 195 microsatellite markers uniformly distributed across the entire genome. For each marker, association with the resistance trait was inferred by measuring in the bulks the ratio of peak intensities of the two alleles inherited from ‘Kishmish vatkana’. The phenotypic locus was assigned to linkage group 13, a genomic region in which no disease resistance had been reported previously. The REN1 position was restricted to a 7.4 cM interval by analyzing the 310 offspring for the segregation of markers that surrounded the target region. The closest markers, VMC9H4-2, VMCNG4E10-1 and UDV-020, were located 0.9 cM away from the REN1 locus.

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Acknowledgments

We thank the Uzbek Research Institute of Horticulture, Viticulture and Enology, Tashkent, Uzbekistan for kindly providing the original plant of ‘Kishmish vatkana’. We also thank A. Pfeiffer for SSR genotyping, A. Szabo for plant growing, and T. Dula for phenotypic tests, W. Qiu for facilitating the transfer of ‘Kishmish vatkana’ to Missouri State University, and J. Howard and C. Coleman for proofreading the manuscript. This research was supported by the Hungarian Scientific Research Fund OTKA K62535, USDA-CSREES Federal Appropriation Grant 2004-38901-02138, and by the Regional Administration of Friuli Venezia Giulia (Italy).

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Authors and Affiliations

  1. Research Institute of Viticulture and Enology, Pázmány Péter u. 4, 7634, Pecs, Hungary

    Sarolta Hoffmann & Pál Kozma

  2. Dipartimento di Scienze Agrarie e Ambientali, University of Udine, via delle Scienze 208, 33100, Udine, Italy

    Gabriele Di Gaspero & Raffaele Testolin

  3. Istituto di Genomica Applicata, Parco Scientifico e Tecnologico Luigi Danieli, via Jacopo Linussio 51, 33100, Udine, Italy

    Gabriele Di Gaspero & Raffaele Testolin

  4. Department of Agriculture, Missouri State University, Mountain Grove, MO, 65711, USA

    László Kovács & Susanne Howard

  5. Institute of Genetics and Biotechnology, Szent István University, Gödöllö, 2100, Hungary

    Erzsébet Kiss & Zsuzsanna Galbács

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  1. Sarolta Hoffmann
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Correspondence to Gabriele Di Gaspero.

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Communicated by H. Nybom.

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122_2007_680_MOESM1_ESM.tif

Figure S1. Microsatellite markers used for bulked segregant analysis. A set of 291 microsatellite markers scattered over the genome was selected in order to evenly cover all linkage groups. Microsatellites belonged to the markers series scu, UDV, VMC, VrZag, VVI, and VVS (Di Gaspero et al., 2007). Marker orders and distances are based on the consensus map of Doligez et al. (2006). Gaps were filled in with additional markers present in the consensus map of Di Gaspero et al. (2007). Each additional marker was projected onto the map of Doligez et al. (2006) in the interval between the surrounding two markers shared by the two maps in a position at the centre center of the interval. No map distance is given for these markers in this map. Bold markers were heterozygous in ‘Kishmish vatkana’ and hence informative for BSA (TIF 6.34 kb)

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Hoffmann, S., Di Gaspero, G., Kovács, L. et al. Resistance to Erysiphe necator in the grapevine ‘Kishmish vatkana’ is controlled by a single locus through restriction of hyphal growth . Theor Appl Genet 116, 427–438 (2008). https://doi.org/10.1007/s00122-007-0680-4

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