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Fine scale genetic and physical mapping using interstitial deletion mutants of Lr34 /Yr18: a disease resistance locus effective against multiple pathogens in wheat

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Abstract

The Lr34/Yr18 locus has contributed to durable, non-race specific resistance against leaf rust (Puccinia triticina) and stripe rust (P. striiformis f. sp. tritici) in wheat (Triticum aestivum). Lr34/Yr18 also cosegregates with resistance to powdery mildew (Pm38) and a leaf tip necrosis phenotype (Ltn1). Using a high resolution mapping family from a cross between near-isogenic lines in the “Thatcher” background we demonstrated that Lr34/Yr18 also cosegregated with stem rust resistance in the field. Lr34/Yr18 probably interacts with unlinked genes to provide enhanced stem rust resistance in “Thatcher”. In view of the relatively low levels of DNA polymorphism reported in the Lr34/Yr18 region, gamma irradiation of the single chromosome substitution line, Lalbahadur(Parula7D) that carries Lr34/Yr18 was used to generate several mutant lines. Characterisation of the mutants revealed a range of highly informative genotypes, which included variable size deletions and an overlapping set of interstitial deletions. The mutants enabled a large number of wheat EST derived markers to be mapped and define a relatively small physical region on chromosome 7DS that carried Lr34/Yr18. Fine scale genetic mapping confirmed the physical mapping and identified a genetic interval of less than 0.5 cM, which contained Lr34/Yr18. Both rice and Brachypodium genome sequences provided useful information for fine mapping of ESTs in wheat. Gene order was more conserved between wheat and Brachypodium than with rice but these smaller grass genomes did not reveal sequence information that could be used to identify a candidate gene for rust resistance in wheat. We predict that Lr34/Yr18 is located within a large insertion in wheat not found at syntenic positions in Brachypodium and rice.

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Acknowledgments

We thank Sutha Chandramohan, Libby Viccars, Kylie Groom and John To for excellent technical assistance. This work was funded by the Grains Research and Development Corporation as a component project of the Australian Winter Cereal Molecular marker Program (grant # CSP00063) to CSIRO and Project CIM13 to CIMMYT. We are also grateful to Karin Deal, Ming Cheng Luo, Sylvie Cloutier, and Boulos Chalhoub for supplying us with BAC clones.

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Authors and Affiliations

  1. CSIRO Plant Industry, GPO Box 1600, Canberra, ACT, 2601, Australia

    W. Spielmeyer, H. McFadden & E. S. Lagudah

  2. CIMMYT, Apdo. Postal 6-641, 06600, Mexico DF, Mexico

    R. P. Singh

  3. Plant Breeding Institute, University of Sydney, Cobbitty, NSW, 2570, Australia

    C. R. Wellings

  4. Campo Experimental Valle de México INIFAP, Apdo. Postal 10, 56230, Chapingo, Edo de México, Mexico

    J. Huerta-Espino

  5. Chinese Academy of Agricultural Science, Beijing, China

    X. Kong

  6. Centre for Comparative Genomics, Murdoch University, Perth, 6150, Australia

    R. Appels

Authors
  1. W. Spielmeyer
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  2. R. P. Singh
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  3. H. McFadden
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  4. C. R. Wellings
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  5. J. Huerta-Espino
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  6. X. Kong
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  7. R. Appels
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  8. E. S. Lagudah
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Corresponding author

Correspondence to E. S. Lagudah.

Additional information

Communicated by R. Waugh.

W. Spielmeyer and R. P. Singh contributed equally to the study through the “Thatcher” and “Lalbahadur” genetic stocks, respectively.

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Spielmeyer, W., Singh, R.P., McFadden, H. et al. Fine scale genetic and physical mapping using interstitial deletion mutants of Lr34 /Yr18: a disease resistance locus effective against multiple pathogens in wheat. Theor Appl Genet 116, 481–490 (2008). https://doi.org/10.1007/s00122-007-0684-0

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  • DOI: https://doi.org/10.1007/s00122-007-0684-0

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