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Genetic mapping and annotation of genomic microsatellites isolated from globe artichoke

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Abstract

Cynara cardunculus includes three taxa, the globe artichoke (subsp. scolymus L. Hegi), the cultivated cardoon (var. altilis) and their progenitor, the wild cardoon (var. sylvestris). Globe artichoke is an important component of the Mediterranean rural economy, but its improvement through breeding has been rather limited and its genome organization remains largely unexplored. Here, we report the isolation of 61 new microsatellite loci which amplified a total of 208 alleles in a panel of 22 C. cardunculus genotypes. Of these, 51 were informative for linkage analysis and 39 were used to increase marker density in the available globe artichoke genetic maps. Sequence analysis of the 22 loci associated with genes showed that 9 are located within coding sequence, with the repetitive domain probably being involved in DNA binding or in protein–protein interactions. The expression of the genes associated with 9 of the 22 microsatellite loci was demonstrated by RT-PCR.

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Acknowledgments

We are very grateful to Prof. Giovanni Mauromicale and Dr. Rosario Mauro, form D.A.C·P.A. – Agronomical Sciences, University of Catania, for having provided most of the C. cardunculus genotypes in study. We also thank Dr. Robert Koebner (http://www.smartenglish.co.uk) for manuscript editing.

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Authors and Affiliations

  1. Di.Va.P.R.A. Plant Genetics and Breeding, University of Turin, via L. da Vinci 44, 10095, Grugliasco (Turin), Italy

    Alberto Acquadro, Sergio Lanteri, Davide Scaglione & Ezio Portis

  2. Plant Research International, P.O. Box 16, 6700 AA, Wageningen, The Netherlands

    Paul Arens & Ben Vosman

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  1. Alberto Acquadro
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  2. Sergio Lanteri
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  3. Davide Scaglione
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  4. Paul Arens
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  5. Ben Vosman
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  6. Ezio Portis
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Correspondence to Ezio Portis.

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Communicated by A. Bervillé.

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Acquadro, A., Lanteri, S., Scaglione, D. et al. Genetic mapping and annotation of genomic microsatellites isolated from globe artichoke. Theor Appl Genet 118, 1573–1587 (2009). https://doi.org/10.1007/s00122-009-1005-6

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  • DOI: https://doi.org/10.1007/s00122-009-1005-6

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