Abstract
Aims/hypothesis
Diabetic cardiomyopathy (DCM) is common in type 2 diabetes. In DCM, insulin resistance may alter cardiac substrate supply and utilisation leading to changes in myocardial metabolism and cardiac function. In rats, exposure to excessive alimentary fat, inducing a type 2 diabetic phenotype, may result in myocardial insulin resistance and cardiac functional changes resembling DCM.
Materials and methods
Rats received high-fat (HFD) or low-fat (LFD) diets for 7 weeks. Prior to killing, insulin or saline was injected i.p. Contractile function and insulin signalling were assessed in papillary muscles and ventricular lysates, respectively.
Results
Fasting and post-load blood glucose levels were increased in HFD- vs LFD-rats (all p<0.02). Mean heart weight, but not body weight, was increased in HFD-rats (p<0.01). HFD-hearts showed structural changes and triglyceride accumulation. HFD-muscles developed higher baseline and maximum forces, but showed impaired recovery from higher workloads. Insulin-associated modulation of Ca2+-induced force augmentation was abolished in HFD-muscles. HFD reduced insulin-stimulated IRS1-associated phosphatidylinositol 3′-kinase activity and phosphorylation of protein kinase B, glycogen synthase kinase-3β, endothelial nitric oxide synthase, and forkhead transcription factors by 40–60% (all p<0.05). Insulin-mediated phosphorylation of phospholamban, a critical regulator of myocardial contractility, was decreased in HFD-hearts (p<0.05).
Conclusions/interpretation
HFD induced a hypertrophy-like cardiac phenotype, characterised by a higher basal contractile force, an impaired recovery from increased workloads and decreased insulin-mediated protection against Ca2+ overload. Cardiac dysfunction was associated with myocardial insulin resistance and phospholamban hypophosphorylation. Our data suggest that myocardial insulin resistance, resulting from exposure to excessive alimentary fat, may contribute to the pathogenesis of diabetes-related heart disease.
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Introduction
Type 2 diabetes is associated with a high incidence of coronary artery disease and congestive heart failure [1, 2]. Even in asymptomatic patients with uncomplicated type 2 diabetes, left ventricular hypertrophy and dysfunction are found [3, 4]. In the absence of coronary artery disease and hypertension, the myocardial abnormalities are ascribed to diabetic cardiomyopathy (DCM) [5]. Changes in myocardial energy metabolism, owing to altered cardiomyocyte substrate supply and utilisation, may underlie the development of DCM [6]. A key mechanism contributing to myocardial dysmetabolic changes may be impaired cardiac insulin signalling [6].
In insulin-resistant and type 2 diabetic subjects, high fluxes of NEFA and triglycerides exist because of unsuppressed lipolysis in adipose tissue and hepatic overproduction of triglyceride-rich particles. This excessive lipid exposure, in the presence of impaired glucose utilisation, results in accumulation of triglycerides in non-adipose tissues, including the myocardium. Inappropriate triglyceride deposition enlarges the intracellular pool of fatty acyl-CoA, thereby providing substrate for non-oxidative metabolic pathways leading to oxidative stress, cellular dysfunction and apoptosis [7]. In animal models of lipotoxicity, myocardial dysfunction was found, which was reversed by anti-steatotic treatment [8]. In vitro studies in cardiomyocytes from Zucker fatty rats suggest that impaired cardiac insulin signalling may underlie the observed abnormalities [9]. These findings are mainly derived from studies in genetic models of insulin resistance and type 2 diabetes, and therefore, it is unclear whether similar mechanisms operate in humans and animal models of alimentary fat-induced insulin resistance, which is believed to be fundamental in the development of human type 2 diabetes.
We investigated whether long-term exposure of normal rats to a high-fat diet (HFD), inducing a type 2 diabetic phenotype, results in myocardial dysfunction and whether these alterations are associated with abnormal myocardial insulin signalling.
Materials and methods
Animals
The investigation conformed to the Guide for the Care and Use of Laboratory Animals as published by the NIH (NIH Publ. No. 85-23, revised 1996) and the regulations of the Institutional Animal Care and Use Committee. Adult male Wistar (WU) rats (n=34; mean body weight 302±6 g; Harlan CBP, Horst, The Netherlands) were fed an HFD for 7 weeks. Animals that received an isocaloric low-fat diet (LFD) for 7 weeks served as controls. At week 6, rats fasted for 6–8 h received an oral glucose load (2 g/kg of body weight). Blood glucose was measured from tail bleeds with a HemoCue glucose analyser (Angelholm, Sweden) at 0, 15, 30, 60, 90 and 120 min after glucose ingestion. After 7 weeks, insulin stimulation was induced in fasted rats through i.p. injection of 10 U/kg body weight insulin (Actrapid 100 U/ml; Novo Nordisk, The Netherlands). Similar insulin levels were achieved in HFD- and LFD-rats. Animals were considered insulin-stimulated when plasma insulin levels exceeded 400 μU/ml. The effects of insulin were compared with those in animals that received a saline injection. Thirty minutes after saline or insulin injection, rats were killed by decapitation, and trunk blood was collected for glucose, insulin, leptin and NEFA determinations. Hearts were removed and transferred to ice-cold buffer containing (in mmol/l) 120 NaCl, 1.22 MgSO4, 1.99 NaH2PO4, 27 NaHCO3, 15 KCl, 1 CaCl2, 10 glucose, and 30 2,3-butanedione 2-monoxime, equilibrated with 95% O2 and 5% CO2. Hearts were perfused via the aorta with the chilled buffer and a papillary muscle with part of the septum and septal artery was dissected from the right ventricle [10]. The remaining ventricular tissue was either snap-frozen in dry-ice-chilled isopentane and stored at −80°C until analysis of insulin signalling, or fixed in Karnovky’s universal fixative (EMS15720) for electron microscopy.
Diets
Experimental diets were obtained from Hope Farms (Woerden, The Netherlands; HFD: cat#4148.02; LFD: cat#4148.01). The LFD consisted of 8 wt% total fat, 22 wt% protein and 60 wt% carbohydrate; the HFD contained 25 wt% fat, 32 wt% protein and 25 wt% carbohydrate, as well as more palmitate (91.12 g/kg) and oleate (100.24 g/kg) compared with the LFD (29.12 and 32.08 g/kg, respectively). In the case of the HFD, 50.4% of the ingested calories were derived from fat compared with 16.4% for the LFD.
In vitro contractile function
Papillary muscles were mounted in a superfusion bath and connected to a force transducer to assess contractile function [10]. The septal artery was cannulated and perfused at 60 cmH2O. The muscle was superfused and perfused by buffer containing (in mmol/l) 118 NaCl, 4.5 MgCl2, 4.5 KCl, 0.33 NaH2PO4, 25 NaHCO3, 1 CaCl2, 10 glucose and 0.01 adenosine, equilibrated with 95% O2 and 5% CO2 and kept at 27°C. Muscles were paced at 0.2 Hz and muscle dimensions were measured to enable normalisation of developed force (F dev) and maximal force (F max) on muscle cross-sectional area. The length of the muscle was set at 95% of the maximal length as determined by a force–length relationship, followed by a stabilisation period of 60 min. Muscles were subjected to a potentiation protocol to determine F max. A force–frequency protocol was applied to increase muscle workload and subsequently recovery of force (F rec) was determined, which is expressed as a percentage of initial force. Finally, to study the effect of Ca2+ on F dev, muscles were exposed to 2 mmol/l extracellular Ca2+.
Plasma determinations
Insulin was measured by RIA (INSIK-5; DiaSorin Biomedica, Saluggia, Italy), which shows 100% cross-reactivity with rat insulin. Plasma NEFA levels were determined using a colorimetric kit (WAKO NEFA-C; Wako Pure Chemical Industries, Osaka, Japan). Serum leptin concentrations were measured by RIA (LEP-R61; Mediagnost, Reutlingen, Germany).
Cardiac lipid analysis
Triglyceride content in ventricular lysates was determined as described previously [11].
Histology and electron microscopy
Frozen sections from liver, skeletal muscle and cardiac ventricular tissue were stained with oil-red-O and counterstained with Mayer’s haematoxylin. For electron microscopy, ultrathin sections of cardiac ventricular tissue were contrasted with uranyl acetate and Reynolds’ lead citrate.
Cardiac insulin signalling
To determine insulin signalling, ventricular tissue was homogenised using an ultraturrax mixer [9]. Lysates were centrifuged (15 min; 14,000 rpm; 4°C), and protein content was determined using a BCA kit (Pierce, Rockford, IL, USA).
The insulin receptor β-subunit, IRS1, and IRS2 were immunoprecipitated using polyclonal insulin receptor β-subunit antibody C19 (Santa Cruz Biotechnology, Santa Cruz, CA, USA), IRS1 antibody K6, and IRS2 antibody K4, respectively [9]. Tyrosine phosphorylation of the immunoprecipitated proteins and IRS1/2-associated phosphatidylinositol 3′-kinase (PI3K) activity were determined as described [9]. Expression and phosphorylation of insulin signalling intermediates were analysed by Western blotting using anti-insulin receptor β-subunit (C19), anti-FOXO3/FKHRL1 (H144), anti-phosphotyrosine-horseradish peroxidase conjugate, anti-endothelial nitric oxide synthase (eNOS; C20), anti-ryanodine receptor (RyR; N19) (Santa Cruz Biotechnology), anti-phospholamban (PLB), anti-PLB-Ser16 (Upstate, Lake Placid, NY, USA), anti-FOXO1/FKHR, anti-glycogen synthase kinase 3β (GSK3β), and phospho-specific protein kinase B (PKB)/Akt-Thr308, PKB/Akt-Ser473, GSK3-Ser9/Ser21, eNOS-Ser1177, Forkhead/FOXO-Thr24, and mammalian target of rapamycin (mTOR)-Ser2448 antibodies (Cell Signaling Technology, Beverly, MA, USA). Polyclonal mTOR has been described [12], whereas PKB/Akt #5178, and sarcoplasmic reticulum Ca2+ ATPase (SERCA2a) antibodies were kindly provided by Dr B. Burgering (UMC Utrecht, The Netherlands) and Dr W. S. Simonides (Laboratory for Physiology, VUMC, Amsterdam, The Netherlands), respectively. Immunoblots were quantified by densitometric analysis of the films.
Statistical analysis
Data are expressed as means±SD or means±SE. Differences between groups were determined by an unpaired Student’s two-tailed t-test. A p value of p<0.05 was considered statistically significant.
Results
HFD induces a non-obese phenotype with impaired glucose tolerance
Mean baseline body weights in LFD- and HFD-rats (302±4.6 and 301±3.7 g, respectively), and overall mean body weight gain during the course of the diet intervention (mean 49±4 days; 132±31 and 131±30 g, respectively) were similar. Fasting (p<0.001) and post-load blood glucose levels (p<0.02) were significantly higher in HFD- than in LFD-rats (Table 1, Fig. 1a). A significant short-term rise in post-load plasma insulin levels was observed in HFD-rats only (p<0.05; Fig. 1b). At killing, no differences in plasma insulin, leptin and NEFA levels were found between the two groups (Table 1). Insulin injection suppressed plasma NEFA levels by 74.4% (p<0.001) in LFD-rats and by 26.7% (p=0.111) in HFD-animals. Only the HFD induced skeletal muscle and liver steatosis (Fig. 1c–f).
HFD-feeding results in cardiac structural and functional abnormalities
Mean heart weight and heart-to-body-weight ratio of HFD-rats were increased as compared with LFD-animals (both p<0.01; Table 1). Electron microscopy revealed marked ultrastructural abnormalities in cardiomyocytes from HFD-rats, in particular degenerative changes of mitochondria, including matrix dilution, cristolysis and mitochondria-associated lamellar bodies (Fig. 2). Biochemical analysis showed a twofold rise in cardiac triglyceride content in HFD- vs LFD-rats (p<0.02; Table 1).
Increases in basal F dev and F max of 1.7- (p<0.01) and 1.6-fold (p<0.05), respectively were observed in papillary muscles obtained from saline-injected HFD-rats, as compared with those from LFD-rats (Fig. 3a,b). Similarly, F dev showed a 1.4-fold increase (p<0.05) in muscles from insulin-injected HFD- vs LFD-animals, but, when compared with the saline-injected groups, the stimulatory effect of insulin on F dev only reached significance in LFD-animals (Fig. 3a). F max was not affected by insulin (Fig. 3b).
Following an enhanced workload, F rec equalled 69.1 and 65.8% of baseline F dev in saline- and insulin-injected LFD- animals, respectively (Fig. 3c), and 36.6 and 47.3% in the corresponding muscles from HFD-rats (both p<0.05), indicating an impaired recovery of HFD muscles. Increasing the buffer Ca2+ concentration to 2 mmol/l induced a rise in F dev in both LFD- and HFD-muscles from saline-injected rats (both p<0.05; Fig. 4a). The Ca2+-induced F dev augmentation was blunted in muscles from insulin-injected LFD-rats (p<0.05), but this insulin-mediated protective effect against Ca2+-overload was abolished in muscles from HFD-rats (Fig. 4a). These findings were not explained by differences in expression levels of sarcoplasmic reticulum Ca2+-cycling proteins RyR and SERCA2a (Fig. 4b). However, a 43% decrease in Ser16-PLB phosphorylation/PLB expression ratio was observed in hearts from insulin-injected HFD-rats (p<0.05 vs LFD; Fig. 4b,c), suggesting an increased inhibitory action of PLB on SERCA2a activity.
HFD reduces activation of multiple myocardial insulin signalling pathways
Phosphorylation of PKB/Akt and its substrates
Insulin markedly increased both Thr308- and Ser473-PKB/Akt phosphorylation in LFD-hearts. PKB/Akt phosphorylation in HFD-hearts was reduced by 64 (Thr308; p<0.02) and 45% (Ser473; p<0.005) relative to LFD, respectively (Fig. 5a,b). Protein expression of PKB/Akt was similar in LFD- and HFD-animals (Fig. 5a). Phosphorylation of the PKB/Akt-substrates eNOS-Ser1177, GSK3β-Ser9 and FOXO1/3-Thr24 was increased in hearts from insulin-injected LFD-rats, but not in HFD-hearts (Fig. 5c). The blunted response to insulin of cardiac mTOR-Ser2448 phosphorylation in HFD- vs LFD-rats did not reach significance (Fig. 5c). Protein expression of the PKB/Akt-substrates did not differ among the experimental groups (Fig. 5d).
Tyrosine phosphorylation of IRS1/IRS2 and associated PI3K activity
Insulin stimulation resulted in a 2.4-fold increase in IRS1 tyrosine phosphorylation in LFD-hearts (Fig. 6a,b), whereas the levels of tyrosine phosphorylated IRS1 were diminished by 57% in HFD-hearts (p<0.001; Fig. 6a,b). Furthermore, insulin induced a 4.7- vs 2.8-fold increase in IRS1-associated PI3K activity in LFD- vs HFD-hearts (p<0.02; Fig. 6a,b). No difference was found in protein expression levels of IRS1 (Fig. 6c). The insulin-induced increase in tyrosine phosphorylation of IRS2 and IRS2-associated PI3K activity failed to reach significance under the experimental conditions used in this study (data not shown).
Tyrosine phosphorylation of the insulin receptor β-subunit
Insulin induced tyrosine phosphorylation of the insulin receptor β-subunit in both groups (Fig. 7a). However, the response to insulin in HFD- vs LFD-rats was reduced by 50% (p<0.05; Fig. 7b). Expression of the insulin receptor protein was similar in both groups (Fig. 7c).
Discussion
Rats fed an HFD developed a type 2 diabetic phenotype, characterised by glucose intolerance and hepatic, skeletal muscle and cardiac steatosis, but with a body weight gain similar to that of LFD controls. HFD-feeding resulted in mild cardiac hypertrophy with higher baseline contractile forces but a markedly diminished recovery after higher workloads. Also, a blunted force-augmentation response to high Ca2+ was observed in insulin-injected HFD-rat papillary muscles, and cardiac ultrastructural abnormalities were found in the HFD-heart only. Furthermore, hearts from insulin-injected HFD-rats showed impaired insulin signalling and PLB hypophosphorylation.
So far, most studies investigating cardiac lipotoxicity have been performed in genetically manipulated rodents, particularly in leptin-deficient or -resistant animals [8, 13]. Therefore, it was unclear whether cardiac lipid accumulation could be induced by mere dietary HFD intake in normal animals. Normal rats fed a 60% HFD for 6 weeks had only a slight elevation of cardiac triglyceride content [7], whereas 10-week-old leptin-deficient ob/ob mice fed standard chow showed a three-fold increase in heart triglyceride content, relative to controls [13]. We found a two-fold increase in cardiac triglyceride content after 7 weeks of HFD-feeding in normal rats, indicating that chronic exposure to a high dietary fat content can indeed induce cardiac lipid accumulation and the associated abnormalities [14, 15].
The observed cardiac hypertrophy in the HFD-rats may be regarded as a first step in the sequence of adaptive responses of the heart to stress caused by a large number of physiological and pathological conditions [16]. Indeed, papillary muscles from the HFD-hearts showed higher basal and maximum forces, but a decreased recovery after higher workload. The underlying mechanism linking HFD-feeding to development of mild cardiac hypertrophy is unclear. On the one hand, hyperinsulinaemia has been implicated in the regulation of cardiac growth [17]. Alternatively, a contribution of triglyceride accumulation and sustained protein kinase C activation through triglyceride metabolites to the hypertrophic changes in HFD-hearts cannot be excluded [18]. Finally, although diet-related blood pressure elevations, contributing to the cardiac hypertrophy, cannot be entirely ruled out, the existence of relevant hypertension in our rats is less likely since no concentric hypertrophy was found (data not shown). Others have shown that blood pressure changes in rats given a 74% fat-containing diet for 17 weeks were unremarkable [19].
Myocardial metabolic changes, i.e. increased NEFA oxidation at the expense of glucose uptake and metabolism, influence cardiac energy status and as such may have contributed to the observed functional alterations in HFD-muscles [20]. Although cardiac metabolism was not assessed, the observed signalling defects as well as the impaired capacity to recover from higher workloads are indicative of an impaired glucose metabolism in HFD-hearts. An increased NEFA supply will not only diminish the rate of glucose metabolism, but will also further increase oxygen consumption both by uncoupling oxidative phosphorylation [21] and by inducing cycling of NEFA in and out of the triglyceride pool, a futile, energy-consuming cycle [22]. Ischaemia is not likely to account for the observed changes since the muscle preparation was continuously perfused with oxygenated buffer [10]. In addition, high levels of NEFA may influence energy consumption related to excitation–contraction coupling as well as other processes related to cellular Ca2+ handling [23, 24]. Therefore, the energetic disadvantage of the predominant use of NEFA as a myocardial energy substrate may result in the reduced ability to recover from a workload challenge.
Associations between myocardial metabolic abnormalities and dysfunction have been reported by some authors [24–27], but not by others [28, 29]. These discrepancies may be the result of differences in animal models and the methods used to assess cardiac function, including substrate availability and the presence or absence of myocardial ischaemia. Insulin may be an important link between myocardial metabolism and function [17, 30, 31]; however, data on insulin-mediated effects and molecular aspects of insulin signalling are largely lacking from the aforementioned studies. Insulin improves cardiac energy efficiency by shifting substrate utilisation towards glucose, and in theory these changes may favourably affect myocardial function, in particular in the hypertrophic heart and under ischaemic conditions. These beneficial effects of insulin were shown both in animals [20], and in humans [32].
HFD-papillary muscles displayed functional insulin resistance, as reflected by the absence of positive inotropy and impaired protection against Ca2+ overload. An altered myocardial contractile response to insulin was previously described in models of diabetes and cardiac hypertrophy [33–37]. Alterations in cardiomyocyte Ca2+ handling in streptozotocin-induced diabetic hearts have been linked to decreased expression and activation of SERCA2a, amongst others through interaction with IRS1/2 [38, 39]. We found no effect of insulin on SERCA2a–IRS1/2 association (data not shown), and, in line with other studies in animal models of insulin resistance/type 2 diabetes, no diet-induced alterations in SERCA2a and RyR expression [40, 41]. However, the observed decrease in Ser16-PLB phosphorylation levels in insulin-injected HFD-hearts may have contributed to the impaired insulin-mediated protection against Ca2+ overload, as a reduction in Ser16-PLB phosphorylation increases the inhibitory action of PLB on SERCA2a activity [42].
At the molecular level, insulin resistance in HFD-hearts was reflected by impaired activation of the insulin receptor and IRS1/PI3K/PKB/Akt-mediated signalling. Although studies regarding insulin receptor activation in the skeletal muscle of type 2 diabetic subjects have yielded conflicting results [43], defects in insulin receptor activation were reported in skeletal muscle from HFD-rats [44] as well as in hearts from leptin-deficient rodents [45, 46]. Various studies have linked NEFA metabolites, like ceramides and diacylglycerol, to activation of protein kinase C, Jun N-terminal kinase, and inhibitor of nuclear factor-κB kinase, which in their turn can abrogate insulin signalling through serine phosphorylation of the insulin receptor and its substrates [14]. We found triglyceride accumulation in skeletal and cardiac muscle from HFD-rats in the presence of unaltered circulating NEFA levels, suggesting that an increased supply of NEFA originating from intramyocellular triglyceride stores, rather than elevated circulating NEFA levels, could be responsible for the observed cardiac insulin resistance. Furthermore, activation of protein phosphatases contributing to impaired insulin signalling in HFD-hearts cannot be ruled out, since preliminary data indicate a slight elevation of protein phosphatase 2A expression in HFD-hearts (data not shown), which has been implicated in the dephosphorylation of, amongst others, PKB/Akt and PLB [47, 48].
The decreased activation of PKB/Akt-mediated signalling in HFD-hearts may result not only in decreased GLUT4-mediated glucose uptake and glycogen synthesis [14], but also in adverse effects on cardiac function. Reduced phosphorylation of eNOS may impair nitric oxide generation and vasodilatation, thereby adversely affecting cardiac function [49]. Finally, here we report for the first time the insulin-mediated phosphorylation of the forkhead transcription factors FOXO1/3 in the heart. Phosphorylation of the forkhead transcription factors FOXO1/3 by PKB/Akt results in their nuclear exclusion and concurrent loss of their gene regulatory function [50]. Although in vitro studies implicate FOXO proteins in cell cycle control, cell survival and apoptosis, their function in the heart clearly depends on future target gene identification.
Taken together, an HFD induced a type 2 diabetic phenotype in rats, cardiac dysfunction and both functional and molecular myocardial insulin resistance. Insulin exerts many different effects that collectively may, directly or indirectly, have a beneficial impact on the heart. However, it still remains to be demonstrated whether insulin can effect these additional actions independently of its metabolic effects. Interventions aimed at ameliorating cardiac insulin signalling may constitute a major therapeutic contribution in the battle against diabetes-related heart disease.
Abbreviations
- DCM:
-
diabetic cardiomyopathy
- eNOS:
-
endothelial nitric oxide synthase
- Fdev :
-
developed force
- Fmax :
-
maximal force
- Frec :
-
recovered force
- FOXO/FKHR:
-
forkhead transcription factor
- GSK3:
-
glycogen synthase kinase 3
- HFD:
-
high-fat diet
- LFD:
-
low-fat diet
- mTOR:
-
mammalian target of rapamycin
- PI3K:
-
phosphatidylinositol 3′-kinase
- PKB/Akt:
-
protein kinase B
- PLB:
-
phospholamban
- RyR:
-
ryanodine receptor
- SERCA2a:
-
sarcoplasmic reticulum Ca2+ ATPase
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Acknowledgements
The authors wish to thank Dr H. Niessen (Department of Pathology, VU University Medical Centre, Amsterdam, The Netherlands) for helpful discussions, and Gerard van der Zon (Department of Molecular Cell Biology, LUMC, The Netherlands) and Maarten Tushuizen (Department of Endocrinology, VU University Medical Centre, Amsterdam, The Netherlands) for technical assistance.
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Ouwens, D.M., Boer, C., Fodor, M. et al. Cardiac dysfunction induced by high-fat diet is associated with altered myocardial insulin signalling in rats. Diabetologia 48, 1229–1237 (2005). https://doi.org/10.1007/s00125-005-1755-x
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DOI: https://doi.org/10.1007/s00125-005-1755-x