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Interaction between uranium and the cytochrome c 3 of Desulfovibrio desulfuricans strain G20

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An Erratum to this article was published on 11 May 2004

Abstract

Cytochrome c 3 of Desulfovibrio desulfuricans strain G20 is an electron carrier for uranium (VI) reduction. When D. desulfuricans G20 was grown in medium containing a non-lethal concentration of uranyl acetate (1 mM), the rate at which the cells reduced U(VI) was decreased compared to cells grown in the absence of uranium. Western analysis did not detect cytochrome c 3 in periplasmic extracts from cells grown in the presence of uranium. The expression of this predominant tetraheme cytochrome was not detectably altered by uranium during growth of the cells as monitored through a translational fusion of the gene encoding cytochrome c 3 (cycA) to lacZ. Instead, cytochrome c 3 protein was found tightly associated with insoluble U(IV), uraninite, after the periplasmic contents of cells were harvested by a pH shift. The association of cytochrome c 3 with U(IV) was interpreted to be non-specific, since pure cytochrome c 3 adsorbed to other insoluble metal oxides, including cupric oxide (CuO), ferric oxide (Fe2O3), and commercially available U(IV) oxide.

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Acknowledgments

This work was supported in part by the Natural and Accelerated Bioremediation Research Program and the Basic Energy Research Program of the US Department of Energy through grants DE-FG02-97ER62495 and DE-FG02-87ER13713, respectively; the Missouri Agricultural Experiment Station; and the Molecular Biology Program, University of Missouri-Columbia.

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Authors and Affiliations

  1. Department of Biochemistry, University of Missouri-Columbia, Columbia, MO, 65211, USA

    Rayford B. Payne, Laurence Casalot, Tessa Rivere, Lise Larsen, Barbara J. Giles & Judy D. Wall

  2. Argonne National Laboratory, 9700 S. Cass Avenue, Argonne, IL, 60439, USA

    Jeffrey H. Terry

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  1. Rayford B. Payne
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  2. Laurence Casalot
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  3. Tessa Rivere
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  6. Barbara J. Giles
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  7. Judy D. Wall
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Correspondence to Judy D. Wall.

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An erratum to this article can be found at http://dx.doi.org/10.1007/s00203-004-0685-1

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Payne, R.B., Casalot, L., Rivere, T. et al. Interaction between uranium and the cytochrome c 3 of Desulfovibrio desulfuricans strain G20. Arch Microbiol 181, 398–406 (2004). https://doi.org/10.1007/s00203-004-0671-7

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  • DOI: https://doi.org/10.1007/s00203-004-0671-7

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