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Gene expression profiling analysis of Mycobacterium tuberculosis genes in response to salicylate

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Abstract

Salicylate stimulates the oxygen consumption and also induces multiple drug resistance in Mycobacterium tuberculosis. To gain insight into the mechanisms involved in the above observations, a microarray analysis of M. tuberculosis genes in response to salicylate was performed. Salicylate, besides highly inducing the 27 kD gene (Rv0560c) previously identified as highly salicylate-inducible, also caused increased transcription of a range of genes including an open reading frame (Rv0559c) that is located immediately downstream of the 27 kD gene, and some membrane/transmembrane proteins that may serve as potential efflux pumps or porins. Salicylate also caused a general shutdown of transcription and translation and energy production by down-regulating a range of genes involved in RNA and protein synthesis and ATP synthesis. The role of the salicylate-regulated genes in salicylate induced drug resistance and its unique effect on stimulating oxygen consumption in tubercle bacillus is discussed.

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Acknowledgments

The work was supported by NIH grant AI-44063 and Natural Science Foundation of China (30328031). The supply of M. tuberculosis arrays by TIGR PFGRC sponsored by NIH is gratefully acknowledged.

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Authors and Affiliations

  1. Department of Molecular Microbiology and Immunology Bloomberg School of Public Health, Johns Hopkins University, 615 N. Wolfe Street, Baltimore, MD, 21205, USA

    Steven Denkin, Sean Byrne & Ying Zhang

  2. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University, Baltimore, MD, 21205, USA

    Charles Jie

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  1. Steven Denkin
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  2. Sean Byrne
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  3. Charles Jie
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  4. Ying Zhang
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Correspondence to Ying Zhang.

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Denkin, S., Byrne, S., Jie, C. et al. Gene expression profiling analysis of Mycobacterium tuberculosis genes in response to salicylate. Arch Microbiol 184, 152–157 (2005). https://doi.org/10.1007/s00203-005-0037-9

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  • DOI: https://doi.org/10.1007/s00203-005-0037-9

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