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The Streptomyces coelicolor dnaK operon contains a second promoter driving the expression of the negative regulator hspR at physiological temperature

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Abstract

HspR (heat shock protein regulator) acts as a negative regulator of different genes in many bacteria. In Streptomyces coelicolor hspR gene is part and the transcriptional repressor of the dnaK operon which encodes the DnaK, GrpE, DnaJ chaperone machines and HspR itself. Our experiments led us to the discovery of a second promoter, internal to dnaK operon, located upstream hspR gene. Transcription from this promoter was detected at 30°C indicating that hspR could play a key physiological role.

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Acknowledgments

We thank Cristina Alaimo who has contributed to preliminary steps of experimental work and Maurizio Noto for his technical support. This work was partially supported by a grant from the Italian Ministero dell’Istruzione dell’Università e della Ricerca (MIUR-ex 60%).

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  1. Paola Salerno

    Present address: Department of Cell and Organism Biology, Lund University, Sölvegatan 35, 223 62, Lund, Sweden

Authors and Affiliations

  1. Dipartimento di Biologia Cellulare e dello Sviluppo, Viale delle Scienze-Parco d’Orleans II, 90128, Palermo, Italy

    Paola Salerno, Sandra Marineo & Anna Maria Puglia

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  1. Paola Salerno
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  2. Sandra Marineo
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  3. Anna Maria Puglia
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Correspondence to Sandra Marineo.

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Communicated by Jean-Luc Pernodet.

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Salerno, P., Marineo, S. & Puglia, A.M. The Streptomyces coelicolor dnaK operon contains a second promoter driving the expression of the negative regulator hspR at physiological temperature. Arch Microbiol 188, 541–546 (2007). https://doi.org/10.1007/s00203-007-0269-y

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