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Enzymes involved in the anoxic utilization of phenyl methyl ethers by Desulfitobacterium hafniense DCB2 and Desulfitobacterium hafniense PCE-S

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Abstract

Phenyl methyl ethers are utilized by Desulfitobacterium hafniense DCB2 and Desulfitobacterium hafniense PCE-S; the methyl group derived from the O-demethylation of these substrates can be used as electron donor for anaerobic fumarate respiration or dehalorespiration. The activity of all enzymes involved in the oxidation of the methyl group to carbon dioxide via the acetyl-CoA pathway was detected in cell extracts of both strains. In addition, a carbon monoxide dehydrogenase activity could be detected. Activity staining of this enzyme indicated that the enzyme is a bifunctional CO dehydrogenase/acetyl-CoA synthase.

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Acknowledgments

This work was supported by a grant from the Deutsche Forschungsgemeinschaft (DFG). We would also like to express our gratitude to Yvonne Greiser for her skillful technical assistance.

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Authors and Affiliations

  1. Institute of Microbiology, Chair of Applied and Ecological Microbiology Friedrich Schiller University Jena, Philosophenweg 12, 07743, Jena, Germany

    Sandra Kreher & Gabriele Diekert

  2. Institute of Microbiology, Christian Albrecht University Kiel, Am Botanischen Garten 1-9, 24118, Kiel, Germany

    Anke Schilhabel

Authors
  1. Sandra Kreher
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  2. Anke Schilhabel
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  3. Gabriele Diekert
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Correspondence to Gabriele Diekert.

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Communicated by Gregory Cook.

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Kreher, S., Schilhabel, A. & Diekert, G. Enzymes involved in the anoxic utilization of phenyl methyl ethers by Desulfitobacterium hafniense DCB2 and Desulfitobacterium hafniense PCE-S. Arch Microbiol 190, 489–495 (2008). https://doi.org/10.1007/s00203-008-0400-8

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  • DOI: https://doi.org/10.1007/s00203-008-0400-8

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