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In vitro anti-inflammatory and pro-aggregative effects of a lipid compound, petrocortyne A, from marine sponges

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Abstract

(3S,14S)-Petrocortyne A, a lipid compound (a C46 polyacetylenic alcohol), from marine sponges (Petrosia sp.) is potently cytotoxic against several solid tumour cells. In this study, we investigated in vitro anti-inflammatory and pro-aggregative effects of petrocortyne A at non-cytotoxic concentrations on various cellular inflammatory phenomena using the macrophage and monocytic cell lines RAW264.7 and U937. Petrocortyne A blocked tumour necrosis factor-α (TNF-α) production strongly and concentration-dependently in lipopolysaccharide (LPS)-activated RAW264.7 cells and phorbol 12-myristate 13-acetate (PMA)/LPS-treated U937 cells. It also blocked NO production concentration-dependently in LPS- or interferon (IFN)-γ-treated RAW264.7 cells. Among the migration factors tested, the compound selectively blocked the expression of hepatocyte growth factor/scatter factor (HGF/SF). On the other hand, as assessed by a cell-cell adhesion assay, petrocortyne A did not block the activation of adhesion molecules induced by aggregative antibodies to adhesion molecules, but suppressed PMA-induced cell-cell adhesion significantly. Intriguingly, petrocortyne A induced U937 homotypic aggregation following long exposure (2 and 3 days), accompanied by weak induction of pro-aggregative signals such as tyrosine phosphorylation of p132 and phosphorylation of extracellular signal-related kinase 1 and 2 (ERK 1/2). Petrocortyne A may thus inhibit cellular inflammatory processes and immune cell migration to inflamed tissue.

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Acknowledgements

This work was supported in part by New Faculty Member Support Grant 3003187-1-1 (2003260) from Kangwon National University (to J. Y. C.).

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Authors and Affiliations

  1. School of Biotechnology and Bioengineering, Kangwon National University, 192-1 Hyoja-2-Dong, Chuncheon, 200-701, Kangwon-Do, South Korea

    Jae Youl Cho

  2. Department of Genetic Engineering, Sungkyunkwan University, 440-746, Suwon, South Korea

    Sungyoul Hong & Sung Hwan Kim

  3. Department of Oral Biology, Oral Science Research Centre, College of Dentistry, Yonsei University, 120-752, Seoul, South Korea

    Sung Hwan Kim

  4. School of Veterinary Medicine, Kyungpook National University, 702-701, Daegu, South Korea

    Man Hee Rhee

  5. College of Pharmacy, Pusan National University, 609-735, Pusan, South Korea

    Ae Ra Kim & Jee H. Jung

  6. Department of Microbiology and Immunology, College of Medicine, Hanyang University, 133-791, Seoul, South Korea

    Taehoon Chun

  7. Department of Pharmacology, College of Medicine, Cheju National University, 690-756, Cheju, South Korea

    Eun Sook Yoo

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  1. Sungyoul Hong
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Correspondence to Jae Youl Cho.

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Hong, S., Kim, S.H., Rhee, M.H. et al. In vitro anti-inflammatory and pro-aggregative effects of a lipid compound, petrocortyne A, from marine sponges. Naunyn-Schmiedeberg's Arch Pharmacol 368, 448–456 (2003). https://doi.org/10.1007/s00210-003-0848-7

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