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Multivariate image analysis of a set of FTIR microspectroscopy images of aged bovine muscle tissue combining image and design information

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Abstract

In this paper we present an algorithm for analysing sets of FTIR microscopic images of tissue sections. The proposed approach allows one to investigate sets of many FTIR tissue images both with respect to sample information (variation from image to image) and spatial variations of tissues (variation within the image). The algorithm is applied to FTIR microscopy images of beef loin muscles containing myofibre and connective tissue regions. The FTIR microscopy images are taken of sub-samples from five different beef loin muscles that were aged for four different lengths of time. The images were investigated regarding variation due to the ageing length and due to the homogeneity of the connective tissue regions. The presented algorithm consists of the following main elements: (1) pre-processing of the spectra to overcome large quality differences in FTIR spectra and differences due to scatter effects, (2) identification of connective tissue regions in every image, (3) labelling of every connective tissue spectrum with respect to its location in the connective tissue region, and (4) analysis of variations in the FTIR microscopic images in regard to ageing time and pixel position of the spectra in the connective tissue region. Important spectral parameters characterising collagen and proteoglycan structure were determined.

Effective optical path length estimated by EMSC

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Acknowledgement

R. Ofstad and A. Kohler are grateful for financial support by the Norwegian Research Council through grant No. 153381/140. The authors thank V. Høst for fine collaboration and Ulrike Böcker and Ganesh Sockalingum for useful discussions.

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Authors and Affiliations

  1. Center for Biospectroscopy and Data Modelling, Norwegian Food Research Institute, Matforsk, Osloveien 1, 1430, Ås, Norway

    A. Kohler, H. Martens, K. Hannesson, C. Kirschner & R. Ofstad

  2. CIGENE Center for Integrative Genetics, Norwegian University of Life Sciences, 1432, Ås, Norway

    A. Kohler & H. Martens

  3. Department of Mathematical Sciences and Technology (IMT), Norwegian University of Life Sciences, 1432, Ås, Norway

    A. Kohler

  4. Unité de Sensométrie et de Chimiométrie, ENITIAA, BP 82225, 44322, Nantes Cedex 3, France

    D. Bertrand

  5. IKBM, Norwegian University of Life Sciences, 1432, Ås, Norway

    H. Martens

  6. University of Copenhagen, Faculty of Life Science, Rolighedsvej 30, 1958, Frederiksberg, Denmark

    H. Martens

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  1. A. Kohler
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  2. D. Bertrand
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  3. H. Martens
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  4. K. Hannesson
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  5. C. Kirschner
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  6. R. Ofstad
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Correspondence to A. Kohler.

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Kohler, A., Bertrand, D., Martens, H. et al. Multivariate image analysis of a set of FTIR microspectroscopy images of aged bovine muscle tissue combining image and design information. Anal Bioanal Chem 389, 1143–1153 (2007). https://doi.org/10.1007/s00216-007-1414-9

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  • DOI: https://doi.org/10.1007/s00216-007-1414-9

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