Skip to main content
SpringerLink
Log in

Modulation of Calcium-dependent Chloride Secretion by Basolateral SK4-like Channels in a Human Bronchial Cell Line

  • Published:
The Journal of Membrane Biology Aims and scope Submit manuscript

Abstract

The human bronchial cell line16HBE14o− was used as a model of airway epithelial cells to study the Ca2+-dependent Cl− secretion and the identity of KCa channels involved in the generation of a favorable driving force for Cl− exit. After ionomycin application, a calcium-activated short-circuit current (I sc) developed, presenting a transient peak followed by a plateau phase. Both phases were inhibited to different degrees by NFA, glybenclamide and NPPB but DIDS was only effective on the peak phase. 86Rb effluxes through both apical and basolateral membranes were stimulated by calcium, blocked by charybdotoxin, clotrimazole and TPA. 1-EBIO, a SK-channel opener, stimulated 86Rb effluxes. Block of basolateral KCa channels resulted in I sc inhibition but, while reduced, I sc was still observed if mucosal Cl− was lowered. Among SK family members, only SK4 and SK1 mRNAs were detected by RT-PCR. KCNQ1 mRNAs were also identified, but involvement of KcAMP channels in Cl− secretion was unlikely, since cAMP application had no effect on 86Rb effluxes. Moreover, chromanol 293B or clofilium, specific inhibitors of KCNQ1 channels, had no effect on cAMP-dependent I sc. In conclusion, two distinct components of Cl− secretion were identified by a pharmacological approach after a Ca 2+i rise. KCa channels presenting the pharmacology of SK4 channels are present on both apical and basolateral membranes, but it is the basolateral SK4-like channels that play a major role in calcium-dependent chloride secretion in 16HBE14o− cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8

Similar content being viewed by others

References

  1. J. Arreola K. Park J.E. Melvin T. Begenisich (1996) ArticleTitleThree distinct chloride channels control anion movements in rat parotid acinar cells. J. Physiol. 490 351–362 Occurrence Handle1:CAS:528:DyaK28XovVOntw%3D%3D Occurrence Handle8821134

    CAS  PubMed  Google Scholar 

  2. J. Arreola J.E. Melvin T. Begenisich (1998) ArticleTitleDifferences in regulation of Ca2+-activated Cl− channels in colonic and parotid secretory cells. Am. J. Physiol. 274 C161–166 Occurrence Handle1:CAS:528:DyaK1cXotFyruw%3D%3D Occurrence Handle9458724

    CAS  PubMed  Google Scholar 

  3. H. Atherton J. Mesher C.T. Poll H. Danahay (2003) ArticleTitlePreliminary pharmacological characterisation of an interleukin-13-enhanced calcium-activated chloride conductance in the human airway epithelium. Naunyn Schmiedebergs Arch. Pharmacol. 367 214–217 Occurrence Handle1:CAS:528:DC%2BD3sXhsVSltr4%3D Occurrence Handle12595965

    CAS  PubMed  Google Scholar 

  4. J. Barhanin F. Lesage E. Guillemare M. Fink M. Lazdunski G. Romey (1996) ArticleTitleK(V)LQT1 and IsK (minK) proteins associate to form the I(Ks) cardiac potassium current. Nature 384 78–80 Occurrence Handle1:CAS:528:DyaK28XmvVegs7c%3D Occurrence Handle8900282

    CAS  PubMed  Google Scholar 

  5. K.E. Barrett S.J. Keely (2000) ArticleTitleChloride secretion by the intestinal epithelium: molecular basis and regulatory aspects. Annu. Rev. Physiol. 62 535–572 Occurrence Handle10.1146/annurev.physiol.62.1.535 Occurrence Handle1:CAS:528:DC%2BD3cXltlGrtr4%3D Occurrence Handle10845102

    Article  CAS  PubMed  Google Scholar 

  6. A.L. Blatz K.L. Magleby (1986) ArticleTitleSingle apamin-blocked Ca2+-activated K+ channels of small conductance in cultured rat skeletal muscle. Nature 323 718–720 Occurrence Handle1:CAS:528:DyaL2sXjtlOj Occurrence Handle2430185

    CAS  PubMed  Google Scholar 

  7. M. Bleich M. Briel A.E. Busch H.J. Lang U. Gerlach H. Gogelein R. Greger K. Kunzelmann (1997) ArticleTitleKVLQT channels are inhibited by the K+ channel blocker 293B. Pfluegers Arch. 434 499–501 Occurrence Handle10.1007/s004240050427 Occurrence Handle1:CAS:528:DyaK2sXks12itL8%3D

    Article  CAS  Google Scholar 

  8. C.T. Bond J. Maylie J.P. Adelman (1999) ArticleTitleSmall-conductance calcium-activated potassium channels. Ann. N. Y. Acad. Sci. 868 370–378 Occurrence Handle1:CAS:528:DyaK1MXjvFKitLc%3D Occurrence Handle10414306

    CAS  PubMed  Google Scholar 

  9. A.E. Busch G.L. Busch E. Ford H. Suessbrich H.J. Lang R. Greger K. Kunzelmann B. Attali W. Stuhmer (1997) ArticleTitleThe role of the IsK protein in the specific pharmacological properties of the IKs channel complex. Br. J. Pharmacol. 122 187–199 Occurrence Handle1:CAS:528:DyaK2sXmtlyntbo%3D Occurrence Handle9313924

    CAS  PubMed  Google Scholar 

  10. L. Butterfield G. Warhurst M.N. Jones G.I. Sandle (1997) ArticleTitleCharacterization of apical potassium channels induced in rat distal colon during potassium adaptation. J. Physiol. 501 537–547 Occurrence Handle1:CAS:528:DyaK2sXkt1eqsLk%3D Occurrence Handle9218214

    CAS  PubMed  Google Scholar 

  11. C. Carignani R. Roncarati R. Rimini G.C. Terstappen (2002) ArticleTitlePharmacological and molecular characterisation of SK3 channels in the TE671 human medulloblastoma cell line. Brain Res. 939 11–18 Occurrence Handle10.1016/S0006-8993(02)02535-0 Occurrence Handle1:CAS:528:DC%2BD38XjvVShu7w%3D Occurrence Handle12020846

    Article  CAS  PubMed  Google Scholar 

  12. T. Chijiwa A. Mishima M. Hagiwara M. Sano I. Hayash T. Inoue K. Naito T. Toshioka H. Hidaka (1990) ArticleTitleInhibition of forskolin-induced neurite outgrowth and protein phosphorylation by a newly synthesized selective inhibitor of cyclic AMP-dependent protein kinase, N-[2-(p-bromocinnamylamino)ethyl]-5-isoquinolinesulfonamide (H-89), of PC12D pheochromocytoma cells. J. Biol. Chem. 265 5267–5272 Occurrence Handle1:CAS:528:DyaK3cXitlaqt7k%3D Occurrence Handle2156866

    CAS  PubMed  Google Scholar 

  13. L.L. Clarke R.C. Boucher (1992) ArticleTitleChloride secretory response to extracellular ATP in human normal and cystic fibrosis nasal epithelia. Am. J. Physiol. 263 C348–C356 Occurrence Handle1:CAS:528:DyaK38XmtFSitL0%3D Occurrence Handle1514583

    CAS  PubMed  Google Scholar 

  14. E.A. Cowley P. Linsdell (2002) ArticleTitleCharacterization of basolateral K+ channels underlying anion secretion in the human airway cell line Calu-3. J. Physiol. 538 747–757 Occurrence Handle10.1113/jphysiol.2001.013300 Occurrence Handle1:CAS:528:DC%2BD38XislGns74%3D Occurrence Handle11826162

    Article  CAS  PubMed  Google Scholar 

  15. A.L. Cozens M.J. Yezzi K. Kunzelmann T. Ohrui L. Chin K. Eng W.E. Finkbeiner J.H. Widdicombe D.C. Gruenert (1994) ArticleTitleCFTR expression and chloride secretion in polarized immortal human bronchial epithelial cells. Am. J. Respir. Cell. Mol. Biol. 10 38–47 Occurrence Handle1:CAS:528:DyaK2cXis1Kms7w%3D Occurrence Handle7507342

    CAS  PubMed  Google Scholar 

  16. S.A. Cunningham M.S. Awayda J.K. Bubien I.I. Ismailov M.P. Arrate B.K. Berdiev D.J. Benos C.M. Fuller (1995) ArticleTitleCloning of an epithelial chloride channel from bovine trachea. J. Biol. Chem. 270 31016–31026 Occurrence Handle10.1074/jbc.270.52.31016 Occurrence Handle1:CAS:528:DyaK28XivFSrtg%3D%3D Occurrence Handle8537359

    Article  CAS  PubMed  Google Scholar 

  17. T.J. Dale J.E. Cryan M.X. Chen D.J. Trezise (2002) ArticleTitlePartial apamin sensitivity of human small conductance Ca2+-activated K+ channels stably expressed in Chinese hamster ovary cells. Naunyn Schmiedebergs Arch. Pharmacol. 366 470–477 Occurrence Handle10.1007/s00210-002-0622-2 Occurrence Handle1:CAS:528:DC%2BD38XnvVOqt7g%3D Occurrence Handle12382077

    Article  CAS  PubMed  Google Scholar 

  18. M.C. Dechecchi A. Tamanini G. Berton G. Cabrini (1993) ArticleTitleProtein kinase C activates chloride conductance in C127 cells stably expressing the cystic fibrosis gene. J. Biol. Chem. 268 11321–11325 Occurrence Handle1:CAS:528:DyaK3sXkt1Kjsro%3D Occurrence Handle7684379

    CAS  PubMed  Google Scholar 

  19. D.C. Devor R.J. Bridge J.M. Pilewski (2000) ArticleTitlePharmacological modulation of ion transport across wild type and ΔF508 CFTR-expressing human bronchial epithelia. Am. J. Physiol. 279 C461–C479 Occurrence Handle1:CAS:528:DC%2BD3cXmtFyjsbw%3D

    CAS  Google Scholar 

  20. D.C. Devor R.A. Frizzell (1993) ArticleTitleCalcium-mediated agonists activate an inwardly rectified K+ channel in colonic secretory cells. Am. J. Physiol. 265 C1271–C1280 Occurrence Handle1:CAS:528:DyaK2cXhtVeisbY%3D Occurrence Handle7694492

    CAS  PubMed  Google Scholar 

  21. D.C. Devor A.K. Singh R.A. Frizzell R.J. Bridge (1996) ArticleTitleModulation of Cl− secretion by benzimidazolone. I. Direct activation of a Ca2+-dependent K+ channel. Am. J. Physiol. 271 L775–L784 Occurrence Handle1:CAS:528:DyaK28XntF2ju7w%3D Occurrence Handle8944721

    CAS  PubMed  Google Scholar 

  22. D.C. Devor A.K. Singh L.C. Lambert A. Deluca R.A. Frizzell R.J. Bridges (1999) ArticleTitleBicarbonate and chloride secretion in Calu-3 human airway epithelial cells. J. Gen. Physiol. 113 743–760 Occurrence Handle10.1085/jgp.113.5.743 Occurrence Handle1:CAS:528:DyaK1MXjtFCrsLc%3D Occurrence Handle10228185

    Article  CAS  PubMed  Google Scholar 

  23. J. Ehrenfeld C. Raschi E. Brochiero (1994) ArticleTitleBasolateral potassium membrane permeability of A6 cells and cell volume regulation. J. Membrane. Biol. 138 181–195 Occurrence Handle1:CAS:528:DyaK2cXisVSrtLo%3D

    CAS  Google Scholar 

  24. C.M. Fuller D.J. Benos (2000) ArticleTitleElectrophysiological characteristics of the Ca2+-activated Cl− channel family of anion transport proteins. Clin. Exp. Pharmacol. Physiol. 27 906–910 Occurrence Handle10.1046/j.1440-1681.2000.03359.x Occurrence Handle1:CAS:528:DC%2BD3cXnvVShtLo%3D Occurrence Handle11071308

    Article  CAS  PubMed  Google Scholar 

  25. C.M. Fuller D.J. Benos (2000) ArticleTitleCa2+-activated Cl− Channels: A newly emerging anion transport family. News Physiol. Sci. 15 165–171 Occurrence Handle1:CAS:528:DC%2BD3cXmslSrsbg%3D Occurrence Handle11390902

    CAS  PubMed  Google Scholar 

  26. R.A. Frizzell (1999) ArticleTitleTen years with CFTR. Physiol Rev. 79 S1–S2 Occurrence Handle1:STN:280:DyaK1M7is1Kisg%3D%3D Occurrence Handle9922373

    CAS  PubMed  Google Scholar 

  27. M.L. Garcia A. Galvez M. Garcia-Calvo V.F. King J. Vazquez G.J. Kaczorowski (1991) ArticleTitleUse of toxins to study potassium channels. J. Bioenerg. Biomembr. 23 615–646 Occurrence Handle1:CAS:528:DyaK3MXltlSrt70%3D Occurrence Handle1917911

    CAS  PubMed  Google Scholar 

  28. U. Gerlach J. Brendel H.J. Lang E.F. Paulus K. Weidmann A. Bruggemann A.E. Busch H. Suessbrich M. Bleich R. Greger (2001) ArticleTitleSynthesis and activity of novel and selective I(Ks)-channel blockers. J. Med. Chem. 44 3831–3837 Occurrence Handle10.1021/jm0109255 Occurrence Handle1:CAS:528:DC%2BD3MXntl2ls70%3D Occurrence Handle11689069

    Article  CAS  PubMed  Google Scholar 

  29. F. Grahammer R. Warth J. Barhanin M. Bleich M.J. Hug (2001) ArticleTitleThe small conductance K+ channel, KCNQ1: expression, function, and subunit composition in murine trachea. J. Biol. Chem. 276 42268–42275 Occurrence Handle10.1074/jbc.M105014200 Occurrence Handle1:CAS:528:DC%2BD3MXosVGmsrc%3D Occurrence Handle11527966

    Article  CAS  PubMed  Google Scholar 

  30. R. Greger M. Bleich N. Riedemann W. Van Driessche D. Ecke R. Warth (1997) ArticleTitleThe role of K+ channels in colonic Cl− secretion. Comp. Biochem. Physiol. A Physiol. 118 271–275 Occurrence Handle10.1016/S0300-9629(96)00304-0 Occurrence Handle1:STN:280:DyaK1c%2Fjt1Omuw%3D%3D Occurrence Handle9366055

    Article  CAS  PubMed  Google Scholar 

  31. B.R. Grubb R.C. Boucher (1999) ArticleTitlePathophysiology of gene-targeted mouse models for cystic fibrosis. Physiol. Rev. 79 S193–S214 Occurrence Handle1:CAS:528:DyaK1MXhs1Orsrs%3D Occurrence Handle9922382

    CAS  PubMed  Google Scholar 

  32. B.R. Grubb R.J. Pickles H. Ye J.R. Yankaskas R.N. Vick J.F. Engelhardt J.M. Wilson L.G. Johnson R.C. Boucher (1994) ArticleTitleInefficient gene transfer by adenovirus vector to cystic fibrosis airway epithelia of mice and humans. Nature 371 802–806 Occurrence Handle10.1038/371802a0 Occurrence Handle1:CAS:528:DyaK2MXitVSisLw%3D Occurrence Handle7523956

    Article  CAS  PubMed  Google Scholar 

  33. A.D. Gruber R.C. Elble H.L. Ji K.D. Schreur C.M. Fuller B.U. Pauli (1998) ArticleTitleGenomic cloning, molecular characterization, and functional analysis of human CLCA1, the first human member of the family of Ca2+-activated Cl− channel proteins. . 54 200–214 Occurrence Handle10.1006/geno.1998.5562 Occurrence Handle1:CAS:528:DyaK1cXnvFWqur0%3D

    Article  CAS  Google Scholar 

  34. A.D. Gruber K.D. Schreur H.L. Ji C.M. Fuller B.U. Pauli (1999) ArticleTitleMolecular cloning and transmembrane structure of hCLCA2 from human lung, trachea, and mammary gland. Am. J. Physiol. 276 C1261–C1270 Occurrence Handle1:CAS:528:DyaK1MXktlSrtbg%3D Occurrence Handle10362588

    CAS  PubMed  Google Scholar 

  35. K.L. Hamilton L. Meads A.G. Butt (1999) ArticleTitle1-EBIO stimulates Cl− secretion by activating a basolateral K+ channel in the mouse jejunum. Pfluegers Arch. 439 158–166 Occurrence Handle10.1007/s004240051140 Occurrence Handle1:CAS:528:DC%2BD3cXhslOlu7w%3D

    Article  CAS  Google Scholar 

  36. J.M. Herbert J.M. Augereau J. Gleye J.P. Maffrand (1990) ArticleTitleChelerythrine is a potent and specific inhibitor of protein kinase C. Biochem. Biophys. Res. Commun. 172 993–999 Occurrence Handle1:CAS:528:DyaK3MXjvVaquw%3D%3D Occurrence Handle2244923

    CAS  PubMed  Google Scholar 

  37. E. Honoré B. Attali G. Romey C. Heurteaux P. Ricard F. Lesage M. Lazdunski J. Barhanin (1991) ArticleTitleCloning, expression, pharmacology and regulation of a delayed rectifier K+ channel in mouse heart. EMBO J. 10 2805–2811 Occurrence Handle1:CAS:528:DyaK38Xlt1yltb8%3D Occurrence Handle1655403

    CAS  PubMed  Google Scholar 

  38. T.M. Ishii C. Silvia B. Hirschberg C.T. Bond J.P. Adelman J. Maylie (1997) ArticleTitleA human intermediate conductance calcium-activated potassium channel. Proc. Natl. Acad. Sci. USA 94 11651–11656 Occurrence Handle10.1073/pnas.94.21.11651 Occurrence Handle1:CAS:528:DyaK2sXmslejt78%3D Occurrence Handle9326665

    Article  CAS  PubMed  Google Scholar 

  39. T. Ishikawa (1996) ArticleTitleA bicarbonate- and weak acid-permeable chloride conductance controlled by cytosolic Ca2+ and ATP in rat submandibular acinar cells. J. Membrane Biol. 153 147–159 Occurrence Handle10.1007/s002329900118 Occurrence Handle1:CAS:528:DyaK28XlslentLw%3D

    Article  CAS  Google Scholar 

  40. H. Jager J.P. Adelman S. Grissmer (2000) ArticleTitleSK2 encodes the apamin-sensitive Ca2+-activated K+ channels in the human leukemic T cell line, Jurkat. FEBS Lett. 469 196–202 Occurrence Handle10.1016/S0014-5793(00)01236-9 Occurrence Handle1:CAS:528:DC%2BD3cXhsFGrtLg%3D Occurrence Handle10713270

    Article  CAS  PubMed  Google Scholar 

  41. C. Jeulin J. Fournier F. Marano A.C. Dazy (2000) ArticleTitleEffects of hydroxyl radicals on outwardly rectifying chloride channels in a cultured human bronchial cell line (16HBE14o−). Pfluegers Arch. 439 331–338 Occurrence Handle10.1007/s004240050947 Occurrence Handle1:CAS:528:DC%2BD3cXhslOltL0%3D

    Article  CAS  Google Scholar 

  42. W.J. Joiner L.Y. Wang M.D. Tang L.K. Kaczmarek (1997) ArticleTitlehSK4, a member of a novel subfamily of calcium-activated potassium channels. Proc. Natl. Acad. Sci. USA 94 11013–11028 Occurrence Handle10.1073/pnas.94.20.11013 Occurrence Handle1:CAS:528:DyaK2sXmtlCmt7k%3D Occurrence Handle9380751

    Article  CAS  PubMed  Google Scholar 

  43. G. Kerst U. Beschorner B. Unsold T. Von Hahn R. Schreiber R. Greger U. Gerlach H.J. Lang K. Kunzelmann M. Bleich (2001) ArticleTitleProperties and function of KCNQ1 K+ channels isolated from the rectal gland of Squalus acanthias. Pfluegers Arch. 443 146–154 Occurrence Handle10.1007/s004240100632 Occurrence Handle1:CAS:528:DC%2BD3MXotVaiurw%3D

    Article  CAS  Google Scholar 

  44. M.R. Knowles L.L. Clarke R.C. Boucher (1991) ArticleTitleActivation by extracellular nucleotides of chloride secretion in the airway epithelia of patients with cystic fibrosis. N. Engl. J. Med. 325 533–538 Occurrence Handle1:CAS:528:DyaK3MXmtVGit7o%3D Occurrence Handle1857389

    CAS  PubMed  Google Scholar 

  45. M. Kohler B. Hirschberg C.T. Bond J.M. Kinzie N.V. Marrion J. Maylie J.P. Adelman (1996) ArticleTitleSmall-conductance, calcium-activated potassium channels from mammalian brain. Science 273 1709–1714 Occurrence Handle8781233

    PubMed  Google Scholar 

  46. T. Koslowsky T. Hug D. Ecke P. Klein R. Greger D.C. Gruenert K. Kunzelmann (1994) ArticleTitleCa2+- and swelling-induced activation of ion conductances in bronchial epithelial cells. Pfluegers Arch. 428 597–603 Occurrence Handle1:CAS:528:DyaK2MXit1Cqurs%3D

    CAS  Google Scholar 

  47. M. Köttgen A. Hoefer S.J. Kim U. Beschorner R. Schreiber M.J. Hug R. Greger (1999) ArticleTitleCarbachol activates a K+ channel of very small conductance in the basolateral membrane of rat pancreatic acinar cells. Pfluegers Arch. 438 597–603 Occurrence Handle10.1007/s004240051082

    Article  Google Scholar 

  48. K. Kunzelmann S. Kathofer A. Hipper D.C. Gruenert R. Gregner (1996) ArticleTitleCulture-dependent expression of Na+ conductances in airway epithelial cells. Pfluegers Arch. 431 78–86 Occurrence Handle10.1007/s004240050038

    Article  Google Scholar 

  49. N. Lalevee M. Joffre (1999) ArticleTitleInhibition by cAMP of calcium-activated chloride currents in cultured Sertoli cells from immature testis. J. Membrane Biol. 169 167–174 Occurrence Handle10.1007/s002329900528 Occurrence Handle1:CAS:528:DyaK1MXjvF2ru78%3D

    Article  CAS  Google Scholar 

  50. W.A. Large Q. Wang (1996) ArticleTitleCharacteristics and physiological role of the Ca2+-activated Cl− conductance in smooth muscle. Am. J. Physiol. 271 C435–C454 Occurrence Handle1:CAS:528:DyaK28XlsV2lurs%3D Occurrence Handle8769982

    CAS  PubMed  Google Scholar 

  51. R. Latorre A. Oberhauser P. Labarca O. Alvarez (1989) ArticleTitleVarieties of calcium-activated potassium channels. Annu. Rev. Physiol. 51 385–399 Occurrence Handle10.1146/annurev.ph.51.030189.002125 Occurrence Handle1:CAS:528:DyaL1MXhvV2rsL4%3D Occurrence Handle2653189

    Article  CAS  PubMed  Google Scholar 

  52. M. Mall T. Gonska J. Thomas R. Schreider H.H. Seydewitz J. Kuehr M. Brandis K. Kunzelmann (2003) ArticleTitleModulation of Ca2+ -activated Cl− secretion by basolateral K+ channels in human normal and cystic fibrosis airway epithelia. Pediatr. Res. 53 608–618 Occurrence Handle10.1203/01.PDR.0000057204.51420.DC Occurrence Handle1:CAS:528:DC%2BD3sXitVKgsLw%3D Occurrence Handle12612194

    Article  CAS  PubMed  Google Scholar 

  53. M. Mall A. Wissner R. Schreiber J. Kuehr H.H. Seydewitz M. Brandis R. Greger K. Kunzelmann (2000) ArticleTitleRole of K(V)LQT1 in cyclic adenosine monophosphate-mediated Cl− secretion in human airway epithelia. Am. J. Respir. Cell Mol. Biol. 23 283–289 Occurrence Handle1:CAS:528:DC%2BD3cXntVSht70%3D Occurrence Handle10970817

    CAS  PubMed  Google Scholar 

  54. A.D. Marmorstein L.Y. Marmorstein M. Rayborn X. Wang J.G. Hollyfield K. Petrukhin (2000) ArticleTitleBestrophin, the product of the Best vitelliform macular dystrophy gene (VMD2), localizes to the basolateral plasma membrane of the retinal pigment epithelium. Proc. Natl. Acad. Sci. USA 97 12758–12763 Occurrence Handle10.1073/pnas.220402097 Occurrence Handle1:CAS:528:DC%2BD3cXotFyls7Y%3D Occurrence Handle11050159

    Article  CAS  PubMed  Google Scholar 

  55. S.J. Mason A.M. Paradiso R.C. Boucher (1991) ArticleTitleRegulation of transepithelial ion transport and intracellular calcium by extracellular ATP in human normal and cystic fibrosis airway epithelium. Br. J. Pharmacol. 103 1649–1656 Occurrence Handle1:CAS:528:DyaK3MXltFaiurg%3D Occurrence Handle1718521

    CAS  PubMed  Google Scholar 

  56. J.D. McCann M.J. Welsh (1990) ArticleTitleBasolateral K+ channels in airway epithelia. II. Role in Cl− secretion and evidence for two types of K+ channel. Am. J. Physiol. 258 L343–L348 Occurrence Handle1:CAS:528:DyaK3cXkslGnsbg%3D Occurrence Handle1694405

    CAS  PubMed  Google Scholar 

  57. A. Nakanishi S. Morita H. Iwashita Y. Sagiya Y. Ashida H. Shirafuji Y. Fujisawa O. Nishimura M. Fujino (2001) ArticleTitleRole of gob-5 in mucus overproduction and airway hyperresponsiveness in asthma. Proc. Natl. Acad. Sci. USA 98 5175–5180 Occurrence Handle10.1073/pnas.081510898 Occurrence Handle1:CAS:528:DC%2BD3MXjt1OnsLw%3D Occurrence Handle11296262

    Article  CAS  PubMed  Google Scholar 

  58. N. Neyroud F. Tesson L. Denjoy M. Leibovici C. Donger J. Barhanin S. Faure F. Gary P. Coumel C. Petit K. Schwartz P. Guicheney (1997) ArticleTitleA novel mutation in the potassium channel gene KVLQT1 causes the Jervell and Lange-Nielsen cardioauditory syndrome. Nature Genet. 15 186–189 Occurrence Handle1:CAS:528:DyaK2sXhtVOlur8%3D Occurrence Handle9020846

    CAS  PubMed  Google Scholar 

  59. B. Nilius J. Prenen G. Szucs L. Wei F. Tanzi T. Voets G. Droogmans (1997) ArticleTitleCalcium-activated chloride channels in bovine pulmonary artery endothelial cells. J. Physiol. 498 381–396 Occurrence Handle1:CAS:528:DyaK2sXhtFCls7k%3D Occurrence Handle9032686

    CAS  PubMed  Google Scholar 

  60. A.M. Paradiso C.M. Ribeiro R.C. Boucher (2001) ArticleTitlePolarized signaling via purinoceptors in normal and cystic fibrosis airway epithelia. J. Gen. Physiol. 117 53–67 Occurrence Handle10.1085/jgp.117.1.53 Occurrence Handle1:CAS:528:DC%2BD3MXltFOktA%3D%3D Occurrence Handle11134231

    Article  CAS  PubMed  Google Scholar 

  61. B.U. Pauli M. Abdel-Ghany H.C. Cheng A.D. Gruber H.A. Archibald R.C. Elble (2000) ArticleTitleMolecular characteristics and functional diversity of CLCA family members. Clin. Exp. Pharmacol. Physiol. 27 901–905 Occurrence Handle10.1046/j.1440-1681.2000.03358.x Occurrence Handle1:CAS:528:DC%2BD3cXnvVShtL0%3D Occurrence Handle11071307

    Article  CAS  PubMed  Google Scholar 

  62. M. Paulmichl J. Pfeilschifter E. Woll F. Lang (1991) ArticleTitleCellular mechanisms of ATP-induced hyperpolarization in renal epithelioid MDCK-cells. J. Cell Physiol. 147 68–75 Occurrence Handle1:CAS:528:DyaK3MXks12ht70%3D Occurrence Handle1903796

    CAS  PubMed  Google Scholar 

  63. P. Pedarzani J. Mosbacher A. Rivard L.A. Cingolani D. Oliver M. Stocker J.P. Adelman B. Fakler (2001) ArticleTitleControl of electrical activity in central neurons by modulating the gating of small conductance Ca2+-activated K+ channels. J. Biol. Chem. 276 9762–9769 Occurrence Handle10.1074/jbc.M010001200 Occurrence Handle1:CAS:528:DC%2BD3MXis1Olu74%3D Occurrence Handle11134030

    Article  CAS  PubMed  Google Scholar 

  64. K.A. Pedersen R.L. Schroder B. Skaaning-Jensen D. Strobaek S.P. Olesen P. Christophersen (1999) ArticleTitleActivation of the human intermediate-conductance Ca2+-activated K+ channel by 1-ethyl-2-benzimidazolinone is strongly Ca2+-dependent. Biochim. Biophys. Acta 1420 231–240 Occurrence Handle10.1016/S0005-2736(99)00110-8 Occurrence Handle1:CAS:528:DyaK1MXlt1Wktr8%3D Occurrence Handle10446306

    Article  CAS  PubMed  Google Scholar 

  65. K. Petrukhin M.J. Koisti B. Bakall W. Li G. Xie T. Marknell O. Sandgren K. Forsman G. Holmgren S. Andreasson M. Vujic A.A. Bergen V. Mcgarty-Dugan D. Figueroa C.P. Austin M.L. Metzker C.T. Caskey C. Wadelius (1998) ArticleTitleIdentification of the gene responsible for Best macular dystrophy. Nature Genet. 19 241–247 Occurrence Handle10.1038/915 Occurrence Handle1:CAS:528:DyaK1cXls1Sns7Y%3D Occurrence Handle9662395

    Article  CAS  PubMed  Google Scholar 

  66. Z. Qu H.C. Hartzell (2001) ArticleTitleFunctional geometry of the permeation pathway of Ca+-activated Cl− channels inferred from analysis of voltage-dependent block. J. Biol. Chem. 276 18423–18429 Occurrence Handle10.1074/jbc.M101264200 Occurrence Handle1:CAS:528:DC%2BD3MXktFWmtL4%3D Occurrence Handle11279188

    Article  CAS  PubMed  Google Scholar 

  67. B.C. Schroeder S. Waldegger S. Fehr M. Bleich R. Warth R. Greger T.J. Jentsch (2000) ArticleTitleA constitutively open potassium channel formed by KCNQ1 and KCNE3. Nature 403 196–199 Occurrence Handle1:CAS:528:DC%2BD3cXmslWmsg%3D%3D Occurrence Handle10646604

    CAS  PubMed  Google Scholar 

  68. E.M. Schwiebert D.J. Benos M.E. Egan M.J. Stutts W.B. Guggino (1999) ArticleTitleCFTR is a conductance regulator as well as a chloride channel. Physiol. Rev. 79 S145–S166 Occurrence Handle1:CAS:528:DyaK1MXhs1Orsrw%3D Occurrence Handle9922379

    CAS  PubMed  Google Scholar 

  69. M. Shah D.G. Haylett (2000) ArticleTitleThe pharmacology of hSK1 Ca2+-activated K+ channels expressed in mammalian cell lines. Br. J. Pharmacol. 129 627–630 Occurrence Handle1:CAS:528:DC%2BD3cXhslWqs70%3D Occurrence Handle10683185

    CAS  PubMed  Google Scholar 

  70. I. Sienaert S. Huyghe J.B. Parys M. Malfait K. Kunzelmann H. De Smedt G.M. Verleden L. Missiaen (1998) ArticleTitleATP-induced Ca2+ signals in bronchial epithelial cells. Pfluegers Arch. 436 40–48 Occurrence Handle10.1007/s004240050602 Occurrence Handle1:CAS:528:DyaK1cXitlyjs70%3D

    Article  CAS  Google Scholar 

  71. S. Singh C.A. Syme A.K. Singh D.C. Devor R.J. Bridges (2001) ArticleTitleBenzimidazolone activators of chloride secretion: potential therapeutics for cystic fibrosis and chronic obstructive pulmonary disease. J. Pharmacol. Exp. Ther. 296 600–611 Occurrence Handle1:CAS:528:DC%2BD3MXosleitw%3D%3D Occurrence Handle11160649

    CAS  PubMed  Google Scholar 

  72. P.L. Smith R.A. Frizzell (1984) ArticleTitleSecretion by canine tracheal epithelium. IV. Basolateral membrane K permeability parallels secretion rate. J. Membrane Biol. 77 187–199 Occurrence Handle1:CAS:528:DyaL2cXotlejsA%3D%3D

    CAS  Google Scholar 

  73. J.N. Snouwaert K.K. Brigman A.M. Latour N.N. Malouf R.C. Boucher O. Smithies B.H. Roller (1992) ArticleTitleAn animal model for cystic fibrosis made by gene targeting. Science. 257 1083–1088 Occurrence Handle1:CAS:528:DyaK38XlvV2ms7c%3D Occurrence Handle1380723

    CAS  PubMed  Google Scholar 

  74. H. Stöhr A. Marquardt I. Nanda M. Schmid B.H. Weber (2002) ArticleTitleThree novel human VMD2-like genes are members of the evolutionary highly conserved RFP-TM family. Eur. J. Hum. Genet. 10 281–284 Occurrence Handle10.1038/sj.ejhg.5200796 Occurrence Handle12032738

    Article  PubMed  Google Scholar 

  75. O. Strauss M. Wiederholt M. Wienrich (1996) ArticleTitleActivation of Cl− currents in cultured rat retinal pigment epithelial cells by intracellular applications of inositol-1,4,5-triphosphate: differences between rats with retinal dystrophy (RCS) and normal rats. J. Membrane Biol. 151 189–200 Occurrence Handle10.1007/s002329900069 Occurrence Handle1:CAS:528:DyaK28XjtlOktb8%3D

    Article  CAS  Google Scholar 

  76. H. Sun T. Tsunenari K.W. Yau J. Nathans (2002) ArticleTitleThe vitelliform macular dystrophy protein defines a new family of chloride channels. Proc. Natl. Acad. Sci. USA 99 4008–4013 Occurrence Handle10.1073/pnas.052692999 Occurrence Handle1:CAS:528:DC%2BD38Xis1Klu70%3D Occurrence Handle11904445

    Article  CAS  PubMed  Google Scholar 

  77. C.A. Syme A.C. Gerlach A.K. Singh D.C. Devor (2000) ArticleTitlePharmacological activation of cloned intermediate- and small-conductance Ca2+-activated K+ channels. Am. J. Physiol. 278 C570–C581 Occurrence Handle1:CAS:528:DC%2BD3cXhvV2qt7k%3D

    CAS  Google Scholar 

  78. J.A. Tabcharani X.B. Chang J.R. Riordan J.W. Hanrahan (1991) ArticleTitlePhosphorylation-regulated Cl− channel in CHO cells stably expressing the cystic fibrosis gene. Nature 352 628–631 Occurrence Handle1:STN:280:By6A38ngvF0%3D Occurrence Handle1714039

    CAS  PubMed  Google Scholar 

  79. T. Takahata M. Hayashi T. Ishikawa (2003) ArticleTitleSK4/IK1-like channels mediate TEA-insensitive, Ca2+-activated K+ currents in bovine parotid acinar cells. Am. J. Physiol. 284 C127–C144 Occurrence Handle1:CAS:528:DC%2BD3sXmvF2qtQ%3D%3D

    CAS  Google Scholar 

  80. T. Takumi H. Ohkubo S. Nakanishi (1988) ArticleTitleCloning of a membrane protein that induces a slow voltage-gated potassium current. Science 242 1042–1045 Occurrence Handle1:CAS:528:DyaL1MXksFWlsLw%3D Occurrence Handle3194754

    CAS  PubMed  Google Scholar 

  81. C. Vergara R. Latorre N.V. Marrion J.P. Adelman (1998) ArticleTitleCalcium-activated potassium channels. Curr. Opin. Neurobiol. 8 321–329 Occurrence Handle10.1016/S0959-4388(98)80056-1 Occurrence Handle1:CAS:528:DyaK1cXktlCju7w%3D Occurrence Handle9687354

    Article  CAS  PubMed  Google Scholar 

  82. J.P. Vincent H. Schweitz M. Lazdunski (1975) ArticleTitleStructure-function relationships and site of action of apamin, a neurotoxic polypeptide of bee venom with an action on the central nervous system. Biochemistry 14 2521–2525 Occurrence Handle1:CAS:528:DyaE2MXltV2msbw%3D Occurrence Handle1138869

    CAS  PubMed  Google Scholar 

  83. T. Von Hahn I. Thiele L. Zingaro K. Hamm M. Garcia-Alzamora M. Kottgen M. Bleich R. Warth (2001) ArticleTitleCharacterisation of the rat SK4/IK1 K+ channel. Cell Physiol. Biochem. 11 219–230 Occurrence Handle10.1159/000051936 Occurrence Handle1:CAS:528:DC%2BD3MXnt1Orsr0%3D Occurrence Handle11509830

    Article  CAS  PubMed  Google Scholar 

  84. J.A. Wagner A.L. Cozens H. Schulman D.C. Gruenert L. Stryer P. Gardner (1991) ArticleTitleActivation of chloride channels in normal and cystic fibrosis airway epithelial cells by multifunctional calcium/calmodulin-dependent protein kinase. Nature 349 793–796 Occurrence Handle10.1038/349793a0 Occurrence Handle1:CAS:528:DyaK3MXhsVSqurk%3D Occurrence Handle1705665

    Article  CAS  PubMed  Google Scholar 

  85. D.E. Walsh B.J. Harvey V. Urbach (2000) ArticleTitleCFTR regulation of intracellular calcium in normal and cystic fibrosis human airway epithelia. J. Membrane Biol. 177 209–219 Occurrence Handle10.1007/s002320010004 Occurrence Handle1:CAS:528:DC%2BD3cXns1Cltb4%3D

    Article  CAS  Google Scholar 

  86. R. Warth M. Bleich (2000) ArticleTitleK+ channels and colonic function. Rev. Physiol. Biochem. Pharmacol. 140 1–62 Occurrence Handle1:CAS:528:DC%2BD3cXkt1ant7w%3D Occurrence Handle10857397

    CAS  PubMed  Google Scholar 

  87. R. Warth K. Hamm M. Bleich K. Kunzelmann T. Von Hahn R. Schreiber E. Ullrich M. Mengel N. Trautmann P. Kindle A. Schwab R. Greger (1999) ArticleTitleMolecular and functional characterization of the small Ca2+-regulated K+ channel (rSK4) of colonic crypts. Pfluegers Arch. 438 437–444 Occurrence Handle10.1007/s004240051059 Occurrence Handle1:CAS:528:DyaK1MXms1ansLs%3D

    Article  CAS  Google Scholar 

  88. M.J. Welsh J.D. McCann (1985) ArticleTitleIntracellular calcium regulates basolateral potassium channels in a chloride-secreting epithelium. Proc. Natl. Acad. Sci. USA 82 8823–8826 Occurrence Handle1:CAS:528:DyaL28Xms1KmsA%3D%3D Occurrence Handle2417229

    CAS  PubMed  Google Scholar 

  89. M.J. Welsh C.M. Liedtke (1986) ArticleTitleChloride and potassium channels in cystic fibrosis airway epithelia. Nature 322 467–470 Occurrence Handle1:CAS:528:DyaL28XltFeisbo%3D Occurrence Handle2426598

    CAS  PubMed  Google Scholar 

  90. H. Wulff M.J. Miller W. Hansel S. Grissmer M.D. Cahalan K.G. Chandy (2000) ArticleTitleDesign of a potent and selective inhibitor of the intermediate-conductance Ca2+ -activated K+ channel, IKCa1: A potential immunosuppressant. Proc. Natl. Acad. Sci. USA 97 8151–8156 Occurrence Handle10.1073/pnas.97.14.8151 Occurrence Handle1:CAS:528:DC%2BD3cXkvFCmsLg%3D Occurrence Handle10884437

    Article  CAS  PubMed  Google Scholar 

  91. W.P. Yang P.C. Levesque W.A. Little M.L. Conder F.Y. Shalaby M.A. Blanar (1997) ArticleTitleKvLQT1, a voltage-gated potassium channel responsible for human cardiac arrhythmias. Proc. Natl. Acad. Sci. USA 94 4017–4021 Occurrence Handle10.1073/pnas.94.8.4017 Occurrence Handle1:CAS:528:DyaK2sXis1anu7Y%3D Occurrence Handle9108097

    Article  CAS  PubMed  Google Scholar 

  92. Y. Zhou Q. Dong I. Louahed C. Dragwa D. Savio M. Huang C. Weiss Y. Tomer M.P. Mclane N.C. Nicolaides R.C. Levitt (2001) ArticleTitleCharacterization of a calcium-activated chloride channel as a shared target of Th2 cytokine pathways and its potential involvement in asthma. Am. J. Respir. Cell Mol. Biol. 25 486–491 Occurrence Handle1:CAS:528:DC%2BD3MXos1eju7o%3D Occurrence Handle11694454

    CAS  PubMed  Google Scholar 

Download references

Acknowledgements

We thank Dr. D.C. Gruenert for kindly providing the 16HBE14o− cells and Dr. P. Durbec for the TE671 cell line and Dr. B. Rossi for Jurkat T cells. This work was supported by the CNRS, the MENRT and the “Vaincre la Mucoviscidose” Association (Paris, France). We thank Dr. S. Schmieder for critical reading of the manuscript.

Author information

Authors and Affiliations

  1. Laboratoire de Physiologie des Membranes cellulaires, Université de Nice-Sophia Antipolis, UMR 6078/CNRS, 06230 Villefranche-sur-Mer, France

    K. Bernard, S. Bogliolo, O. Soriani & J. Ehrenfeld

Authors
  1. K. Bernard
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. Bogliolo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. O. Soriani
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. J. Ehrenfeld
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to J. Ehrenfeld.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bernard, K., Bogliolo, S., Soriani, O. et al. Modulation of Calcium-dependent Chloride Secretion by Basolateral SK4-like Channels in a Human Bronchial Cell Line . J. Membrane Biol. 196, 15–31 (2003). https://doi.org/10.1007/s00232-003-0621-3

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00232-003-0621-3

Keywords

Search

Navigation