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Bacteria Isolated from the Different Developmental Stages and Larval Organs of the Obligate Parasitic Fly, Wohlfahrtia magnifica (Diptera: Sarcophagidae)

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Abstract

Wohlfahrtia magnifica (Diptera: Sarcophagidae) is the major myiasis-causing fly species in the whole of Eurasia for most important domestic animals. The aim of the present work was to obtain data on the culturable bacteria isolated under aerobic conditions from this fly: bacteria were isolated from all developmental stages (larvae, pupa, and imago) of Wohlfahrtia magnifica, and the third-stage larval organs were also sampled. To determine the possible antagonistic effects between the dominant bacterial groups, an antibiosis assay was carried out. Plating and isolation of bacteria was performed by classical microbiological methods. Characterization of the isolated strains was carried out via a polyphasic approach; classical phenotypic tests, chemotaxonomical examinations, and 16S rDNA sequence analyses were also applied. In the case of maggot macerate samples, members of the family Enterobacteriaceae were characteristic. Members of a new genus (Schineria) belonging to the γ subdivision of proteobacteria were also isolated. According to our data, the shifts in the Schineria and Proteus populations within the larvae are strongly influenced by their interactions with each other and among the members of the family Enterobacteriaceae. The pupa and imago samples contained several other Gram-negative bacteria (Stenotrophomonas, Brevundimonas, etc.). Among Gram-positive bacteria, in all maggot macerate samples, members of the genus Bacillus and the Arthrobacter–Micrococcus group of actinobacteria were dominant (neither of them was a producer or sensitive to the compounds of other microorganisms), and bacteria related to the genus Corynebacterium were also found. From the larvae Aureobacterium liquefaciens and Enterococcus faecalis were isolated, and from the pupae Dietzia maris and Enterococcus faecalis. In the samples of third-stage larval organs, the dominant groups were the same as in the third-stage larval macerate sample; however, several additional genera/species were observed (Rhodococcus fascians, Streptomyces sp., Rathayibacter sp., Bacillus thuringiensis/cereus).

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Authors and Affiliations

  1. Department of Microbiology, Faculty of Science, Eötvös Loránd University, Pázmány P. sétány 1/c, H-1117, Budapest, Hungary

    E. M. Tóth, É. Hell, G. Kovács, A. K. Borsodi & K. Márialigeti

  2. Department of Parasitology and Zoology, Faculty of Veterinary Science, Szent István University, Budapest, Hungary

    É. Hell

  3. National Public Health and Health Officer's Service, Budapest, Hungary

    G. Kovács

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  1. E. M. Tóth
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  2. É. Hell
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  3. G. Kovács
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  5. K. Márialigeti
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Correspondence to E. M. Tóth.

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Tóth, E.M., Hell, É., Kovács, G. et al. Bacteria Isolated from the Different Developmental Stages and Larval Organs of the Obligate Parasitic Fly, Wohlfahrtia magnifica (Diptera: Sarcophagidae). Microb Ecol 51, 13–21 (2006). https://doi.org/10.1007/s00248-005-0090-6

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