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Extensive sharing of MHC class II alleles between rhesus and cynomolgus macaques

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Abstract

In contrast to rhesus monkeys, substantial knowledge on cynomolgus monkey major histocompatibility complex (MHC) class II haplotypes is lacking. Therefore, 17 animals, including one pedigreed family, were thoroughly characterized for polymorphic Mhc class II region genes as well as their mitochondrial DNA (mtDNA) sequences. Different cynomolgus macaque populations appear to exhibit unique mtDNA profiles reflecting their geographic origin. Within the present panel, 10 Mafa-DPB1, 14 Mafa-DQA1, 12 Mafa-DQB1, and 35 Mafa-DRB exon 2 sequences were identified. All of these alleles cluster into lineages that were previously described for rhesus macaques. Moreover, about half of the Mafa-DPB1, Mafa-DQA1, and Mafa-DQB1 alleles and one third of the Mafa-DRB exon 2 sequences are identical to rhesus macaque orthologues. Such a high level of Mhc class II allele sharing has not been reported for primate species. Pedigree analysis allowed the characterization of nine distinct Mafa class II haplotypes, and seven additional ones could be deduced. Two of these haplotypes harbor a duplication of the Mafa-DQB1 locus. Despite extensive allele sharing, rhesus and cynomolgus monkeys do not appear to possess identical Mhc class II haplotypes, thus illustrating that new haplotypes were generated after speciation by recombination-like processes.

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Acknowledgements

The authors wish to thank Ms. Donna Devine for editing the manuscript and Henk van Westbroek for preparing the figures. This study was supported, in part, by the National Institutes of Health (NIH) project 1-R24-RR16038-01 (Catalog of Federal Domestic Assistance 93.306) and the NIH/National Center for Research Resources project U24-RR18144-01. The experiments complied with relevant current laws of The Netherlands.

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Authors and Affiliations

  1. Department of Comparative Genetics and Refinement, Biomedical Primate Research Centre, Rijswijk, The Netherlands

    Gaby G. M. Doxiadis, Annemiek J. M. Rouweler, Natasja G. de Groot, Nel Otting & Ronald E. Bontrop

  2. Department of Animal Science, Biomedical Primate Research Centre, Rijswijk, The Netherlands

    Annet Louwerse

  3. Department of Virology, Biomedical Primate Research Centre, Rijswijk, The Netherlands

    Ernst J. Verschoor

  4. Biomedical Primate Research Centre, P.O. Box 3306, 2280 GH, Rijswijk, The Netherlands

    Gaby G. M. Doxiadis

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  1. Gaby G. M. Doxiadis
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Correspondence to Gaby G. M. Doxiadis.

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Doxiadis, G.G.M., Rouweler, A.J.M., de Groot, N.G. et al. Extensive sharing of MHC class II alleles between rhesus and cynomolgus macaques. Immunogenetics 58, 259–268 (2006). https://doi.org/10.1007/s00251-006-0083-8

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