Abstract
Cigarette smoke components have a proven negative influence on human health. Adverse metabolic effects were observed in tissues and single cells. T lymphocytes get in contact with affected organs (e.g., lung) or cells (e.g., erythrocytes), as well as with smoke components and bioactive molecules, whose production is triggered by tobacco smoke. We therefore compared the gene expression profiles in these cells of the adaptive immune system of three male heavy smokers and three male nonsmokers using rapid T cell isolation and Affymetrix GeneChip® HG U133A 2.0 microarray analysis. Eighty-eight genes were found to be significantly (t test) differentially expressed by a factor of 1.5-fold or larger between smokers and nonsmokers. Using the gene function groups of the gene ontology consortium to categorize the functions of the differentially expressed genes, the group termed “response to stimulus” was found to be most significantly affected by smoking. Our data indicate a prominent role of cytotoxic T lymphocytes in response to smoking. Several genes that are typically expressed in these cells were found regulated although the ratio of cytotoxic and helper T lymphocytes remained unchanged in smokers. Our data show that, in principle, it might be possible to identify health-related biomarkers in the transcriptome of T lymphocytes.
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Abbreviations
- CEL:
-
cell intensity file
- CSC:
-
cigarette smoke components
- EDTA:
-
ethylenediaminetetraacetic acid
- FACS:
-
fluorescence-activated cell sorting
- GCOS:
-
GeneChip Operating Software
- GEO:
-
Gene Expression Omnibus
- GEP:
-
gene expression profile
- GO:
-
gene ontology
- NK cell:
-
natural killer cell
- PBMC:
-
peripheral blood mononuclear cell
- PBS:
-
phosphate-buffered saline
References
Andersson A, Qvarfordt I, Laan M, Sjostrand M, Malmhall C, Riise GC et al (2004) Impact of tobacco smoke on interleukin-16 protein in human airways, lymphoid tissue and T lymphocytes. Clin Exp Immunol 138(1):75–82
Ashburner M, Ball CA, Blake JA, Botstein D, Butler H, Cherry JM et al (2000) Gene ontology: tool for the unification of biology. The Gene Ontology Consortium. Nat Genet 25(1):25–29
Grossman WJ, Verbsky JW, Tollefsen BL, Kemper C, Atkinson JP, Ley TJ (2004) Differential expression of granzymes A and B in human cytotoxic lymphocyte subsets and T regulatory cells. Blood 104(9):2840–2848
Lambert C, McCue J, Portas M, Ouyang Y, Li J, Rosano TG et al (2005) Acrolein in cigarette smoke inhibits T-cell responses. J Allergy Clin Immunol 116(4):916–922
Lampe JW, Stepaniants SB, Mao M, Radich JP, Dai H, Linsley PS et al (2004) Signatures of environmental exposures using peripheral leukocyte gene expression: tobacco smoke. Cancer Epidemiol Biomarkers Prev 13(3):445–453
Larkin JE, Frank BC, Gavras H, Sultana R, Quackenbush J (2005) Independence and reproducibility across microarray platforms. Nat Methods 2(5):337–344
McMahon CW, Raulet DH (2001) Expression and function of NK cell receptors in CD8+ T cells. Curr Opin Immunol 13(4):465–470
Moszczynski P, Zabinski Z, Moszczynski P Jr, Rutowski J, Slowinski S, Tabarowski Z (2001) Immunological findings in cigarette smokers. Toxicol Lett 118(3):121–127
Ockene IS, Miller NH (1997) Cigarette smoking, cardiovascular disease, and stroke: a statement for healthcare professionals from the American Heart Association. American Heart Association Task Force on Risk Reduction. Circulation 96(9):3243–3247
Schaberg T, Theilacker C, Nitschke OT, Lode H (1997) Lymphocyte subsets in peripheral blood and smoking habits. Lung 175(6):387–394
Stievano L, Tosello V, Marcato N, Rosato A, Sebelin A, Chieco-Bianchi L et al (2003) CD8+ alpha beta+ T cells that lack surface CD5 antigen expression are a major lymphotactin (XCL1) source in peripheral blood lymphocytes. J Immunol 171(9):4528–4538
van Leeuwen DM, Gottschalk RW, van Herwijnen MH, Moonen EJ, Kleinjans JC, van Delft JH (2005) Differential gene expression in human peripheral blood mononuclear cells induced by cigarette smoke and its constituents. Toxicol Sci 86(1):200–210
Williams H, Macsween K, McAulay K, Higgins C, Harrison N, Swerdlow A et al (2004) Analysis of immune activation and clinical events in acute infectious mononucleosis. J Infect Dis 190(1):63–71
Wu Z, Irizarry RA, Gentleman R, Martinez Murillo F, Spencer F (2003) A Model based background adjustment for oligonucleotide expression arrays. JASA 99:909–917
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This work was supported by the Bundesministerium für Bildung und Forschung. The experiments comply with the laws of Germany.
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Büttner, P., Mosig, S. & Funke, H. Gene expression profiles of T lymphocytes are sensitive to the influence of heavy smoking: a pilot study. Immunogenetics 59, 37–43 (2007). https://doi.org/10.1007/s00251-006-0177-3
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DOI: https://doi.org/10.1007/s00251-006-0177-3