Abstract
Aeromonas hydrophila CGMCC 0911 isolated from lake water was found to be able to synthesize a polyhydroxyalkanoate (PHA) copolymer (PHBHHx) consisting of 3-hydroxybutyrate (HB) and 4–6 mol% 3-hydroxyhexanoate (HHx). The wild-type bacterium accumulated 49% PHBHHx containing 6 mol% HHx in terms of cell dry weight (CDW) when grown on lauric acid for 48 h. When A. hydrophila CGMCC 0911 expressed the Acyl-CoA dehydrogenase gene (yafH) of Escherichia coli, the recombinant strain could accumulate 47% PHBHHx, while the HHx content reached 17.4 mol%. The presence of changing glucose concentration in the culture changed the HHx content both in wild type and recombinant A. hydrophila CGMCC 0911. When 5 g l−1 glucose was added to a culture containing 5 g l−1 lauric acid as co-substrate, 45% PHBHHx/CDW consisting of 8.8 mol% HHx was produced by wild-type A. hydrophila CGMCC 0911 compared with only 5% in the absence of glucose. When the recombinant A. hydrophila CGMCC 0911 was grown on a mixed substrate containing lauric acid and 8–10 g l−1 glucose, the HHx content could be further increased to 35.6 mol%. When the glucose concentration exceeded 10 g l−1, cell growth, PHA content and mole percentages of HHx in PHBHHx were significantly reduced.
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Acknowledgements
This research was supported by Natural Sciences Foundation of China Grant No. 30170017 and No. 20074020. The State 863 funds also provided financial contribution for this study. We are very grateful for the plasmid pBBR1MCS-2(R.e) provided by Dr. Philip Green of Procter & Gamble (Cincinnati, Ohio).
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Lu, X.Y., Wu, Q. & Chen, G.Q. Production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) with flexible 3-hydroxyhexanoate content in Aeromonas hydrophila CGMCC 0911. Appl Microbiol Biotechnol 64, 41–45 (2004). https://doi.org/10.1007/s00253-003-1417-1
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DOI: https://doi.org/10.1007/s00253-003-1417-1