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Purification and characterization of laccase from Pycnoporus sanguineus and decolorization of an anthraquinone dye by the enzyme

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Abstract

The white rot fungus Pycnoporus sanguineus produced high amount of laccase in the basal liquid medium without induction. Laccase was purified using ultrafiltration, anion-exchange chromatography, and gel filtration. The molecular weight of the purified laccase was estimated as 61.4 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The enzyme oxidized typical substrates of laccases including 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonate), 2,6-dimethoxyphenol, and syringaldazine. The optimum pH and temperature for the purified laccase were 3.0 and 65°C, respectively. The enzyme was stable up to 40°C, and high laccase activity was maintained at pH 2.0–5.0. Sodium azide, l-cysteine, and dithiothreitol strongly inhibited the laccase activity. The purified enzyme efficiently decolorized Remazol Brilliant Blue R in the absence of added redox mediators. The high production of P. sanguineus laccase as well as its decolorization ability demonstrated its potential applications in dye decolorization.

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Authors and Affiliations

  1. College of Life Sciences, Northeast Forestry University, No. 26, Hexing Road, Harbin, Heilongjiang, 150040, People’s Republic of China

    Lei Lu, Min Zhao, Bei-Bei Zhang, Shu-Yu Yu, Xi-Jun Bian & Wei Wang

  2. Department of Applied Chemistry, Harbin Institute of Technology, No. 92, West Da-Zhi Street, Harbin, Heilongjiang, 150001, People’s Republic of China

    Yan Wang

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  1. Lei Lu
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  2. Min Zhao
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  3. Bei-Bei Zhang
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  4. Shu-Yu Yu
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  5. Xi-Jun Bian
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  6. Wei Wang
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  7. Yan Wang
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Correspondence to Yan Wang.

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Lu, L., Zhao, M., Zhang, BB. et al. Purification and characterization of laccase from Pycnoporus sanguineus and decolorization of an anthraquinone dye by the enzyme. Appl Microbiol Biotechnol 74, 1232–1239 (2007). https://doi.org/10.1007/s00253-006-0767-x

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  • DOI: https://doi.org/10.1007/s00253-006-0767-x

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