Abstract
Today, biomass covers about 10% of the world’s primary energy demand. Against a backdrop of rising crude oil prices, depletion of resources, political instability in producing countries and environmental challenges, besides efficiency and intelligent use, only biomass has the potential to replace the supply of an energy hungry civilisation. Plant biomass is an abundant and renewable source of energy-rich carbohydrates which can be efficiently converted by microbes into biofuels, of which, only bioethanol is produced on an industrial scale today. Biomethane is produced on a large scale, but is not yet utilised for transportation. Biobutanol is on the agenda of several companies and may be used in the near future as a supplement for gasoline, diesel and kerosene, as well as contributing to the partially biological production of butyl-t-butylether, BTBE as does bioethanol today with ETBE. Biohydrogen, biomethanol and microbially made biodiesel still require further development. This paper reviews microbially made biofuels which have potential to replace our present day fuels, either alone, by blending, or by chemical conversion. It also summarises the history of biofuels and provides insight into the actual production in various countries, reviewing their policies and adaptivity to the energy challenges of foreseeable future.
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References
Alterthum F, Ingram LO (1989) Efficient ethanol production from glucose, lactose, and xylose by recombinant Escherichia coli. Appl Environ Microbiol 55:1943–1948
Anonymous (2004) Biomethanol from sugar beat pulp. Energy & Sustainable Development Magazine, No. 3, p 15
Ariesyady HD, Ito T, Okabe S (2007) Functional bacterial and archaeal community structures of major trophic groups in a full-scale anaerobic sludge digester. Water Res 41:1554–1568
Brooks TA, Ingram LO (1995) Conversion of mixed waste office paper to ethanol by genetically engineered Klebsiella oxytoca strain P2. Biotechnol Prog 11:619–625
Cheng KK, Zhang JA, Liu DH, Sun Y, Liu HJ, Yang MD, Xu JM (2007) Pilot-scale production of 1,3-propanediol using Klebsiella pneumoniae. Process Biochem 42:740–744
Cirne DG, Lehtomäki A, Björnsson L, Blackall LL (2007) Hydrolysis and microbial community analyses in two-stage anaerobic digestion of energy crops. J Appl Microbiol 103:516–527
Claassen PAM, de Vrije T, Budde MAW (2004) Biological hydrogen production from sweet sorghum by thermopilic bacteria. Proceedings 2nd World Conference on Biomass for Energy, Rome, pp 1522–1525
Das D, Verziroglu TN (2001) Hydrogen production by biological processes: a survey of literature. Int J Hydrogen Energy 26:13–28
de Vrije T, Claassen PAM (2003) Dark hydrogen fermentations. In: Reith JH, Wijffels RH, Barten H (eds) Dutch Biological Hydrogen Foundation, Petten, pp 103–123
de Vrije T, de Haas GG, Tan GB, Keijsers ERP, Claassen PAM (2002) Pretreatment of Miscanthus for hydrogen production by Thermotoga elfii. Int J Hydrogen Energy 27:1381–1390
Demain AL, Newcomb M, Wu JHD (2005) Cellulase, clostridia, and ethanol. Microbiol Mol Biol Rev 69:124–154
Demirbas A (2007) Progress and recent trends in biofuels. Progr Energy Combust Sci 33:1–18
EIA (2006) International energy: outlook. Energy Information Administration, Office of Integrated Analysis and Forecasting. US Department of Energy, Washington, DOE/EIA-0484
Einspanier R, Lutz B, Rief S, Berezina O, Zverlov V, Schwarz WH, Mayer J (2004) Tracing residual recombinant feed molecules during digestion and rumen bacterial diversity in cattle fed transgene maize. Eur Food Res Technol 218:269–273
Esper B, Badura A, Rögner M (2006) Photosynthesis as a power supply for (bio-) hydrogen production. Trends Plant Sci 11:543–549
Eurostat (2007) Online Database of the European Union: http://epp.eurostat.ec.europa.eu, Eurostat, 2920 Luxembourg, 08.05.2007
Finlay MR (2004) Old efforts at new uses: a brief history of chemurgy and the American search for biobased materials. J Ind Ecol 7:33–46
FNR (2007) Fachagentur Nachwachsende Rohstoffe e.V.: Biokraftstoffe, online database: http://www.fnr-server.de/cms35/Biokraftstoffe.817.0.html, D-Gülzow, 10.05.2007
Gapes JR (2000) The economics of acetone–butanol fermentation: theoretical and market considerations. J Mol Microbiol Biotechnol 2:27–32
Gapes JR, Gapes RF (2007) Relevance & economics of a biodiesel/biofuels industry. Vision 20/20, IPENZ Annual Conference, Auckland, New Zealand, 23 March, 2007
Gassen HG (2005) Ein Beitrag zur umweltfreundlichen Energieversorgung: Biogasanlagen. Biol in Unserer Zeit 6:384–392
Giebelhaus AW (1980) Farming for fuel: the alcohol motor fuel movement in the 1930s. Agric Hist 54:173–184
Golias H, Dumsday GJ, Stanley GA, Pamment NB (2002) Evaluation of a recombinant Klebsiella oxytoca strain for ethanol production from cellulose by simultaneous saccharification and fermentation: comparison with native cellobiose-utilising yeast strains and performance in co-culture with thermotolerant yeast and Zymomonas mobilis. J Biotechnol 96:155–168
Gray KA, Zhao L, Emptage M (2006) Bioethanol. Curr Opin Chem Biol 10:141–146
GTZ (2005) German technical cooperation: liquid biofuels for transportation in Tanzania—potential and implications for sustainable agriculture and energy in the 21st century, August 2005
Hahn-Hägerdahl B, Karhumaa K, Fonseca C, Spencer-Martins I, Gorwa-Grauslund MF (2007) Towards industrial pentose-fermenting yeast strains. Appl Microbiol Biotechnol 74:937–953
Hayn M, Steiner W, Klinger R, Steinmüller H, Sinner M, Esterbauer H (1993) Basic research and pilot studies on the enzymatic conversion of lignocellulosics. In: Saddler JN (ed) Bioconversion of forest and agricultural residue. Biotechnology in agriculture series, no. 9. CAB International, Wallingford, UK, pp 33–72
Henstra AM, Sipma J, Rinzema A, Stams AJM (2007) Microbiology of synthesis gas fermentation for biofuel production. Curr Opin Biotechnol 18:1–7 (corrected proof, available online 30 March 2007)
Hill J, Nelson E, Tilman D, Polasky S, Tiffany D (2006) Environmental, economic, and energetic costs and benefits of biodiesel and ethanol biofuels. Proc Natl Acad Sci 103:11206–11210
IEA (2004) Biofuels for transport: an international perspective. International Energy Agency, Paris
Ingram LO, Doran JB (1995) Conversion of cellulosic materials to ethanol. FEMS Microbiol Rev 16:235–241
IPCC; Houghton JT, Ding Y, Griggs DJ, Noguer M, van der Linden PJ, Xiaosu D (eds) (2001) Climate Change 2001: the scientific basis. Contribution of Working Group I to the Third Assessment Report of the Intergovernmental Panel on Climate Change (IPCC). Cambridge University Press, UK, p 944
Jones DT, Woods DR (1986) Acetone–butanol fermentation revisited. Microbiol Rev 50:484–524
Judd B (2003) Feasibility of producing diesel fuels from biomass in New Zealand. Energy Efficiency and Conservation Authority, New Zealand, June 2003 online: http://eeca.govt.nz/eeca-library/renewable-energy/bioenergy/report/feasibility-of-producing-diesel-fuels-from-biomass-in-nz-03.pdf
Kalscheuer R, Stölting T, Steinbüchel A (2006) Microdiesel: Escherichia coli engineered for fuel production. Microbiology 152:2529–2536
Kildiran G, Yücel SÖ, Türkay S (1996) In-situ alcoholysis of soybean oil. JAOCS 73:225–228
Klocke M, Mähnert P, Mundt K, Souidi K, Linke B (2007) Microbial community analysis of a biogas-producing completely stirred tank reactor fed continuously with fodder beet silage as mono-substrate. Syst Appl Microbiol 30:139–151
Kovarik B (1998) Henry Ford, Charles Kettering, and the “fuel of the future.” Automot Hist Rev 32:7–27. Reproduced at http://www.radford.edu/_wkovarik/papers/fuel.html
Lapuerta M, Armas O, Garcia-Contreras R (2007) Stability of diesel–bioethanol blends for use in diesel engines. Fuel 86:1351–1357
Lynd LR, Weimer PJ, van Zyl WH, Pretorius IS (2002) Microbial cellulose utilization: fundamentals and biotechnology. Microbiol Mol Biol Rev 66:506–577
Ma F, Hanna MA (1999) Biodiesel production: a review. Bioresour Technol 70:1–15
Malhotra R (2007) Road to emerging alternatives—biofuels and hydrogen. Journal of the Petrotech Society 4:34–40
Moniruzzaman M, Dien BS, Ferrer B, Hespell RB, Dale BE, Ingram LO, Bothast RJ (1996) Ethanol production from AFEX pretreated corn fiber by recombinant bacteria. Biotechnol Lett 18:985–990
Nimcevic D, Gapes JR (2000) The acetone-butanol fermentation in pilot plant and pre-industrial scale. J Mol Microbiol Biotechnol 2:15–20
Nimcevic D, Puntigam R, Wörgetter M, Gapes JR (2000) Preparation of rapeseed oil esters of lower aliphatic alcohols. JAOCS 77:275–280
O’Sullivan CA, Burrell PC, Clarke WP, Blackall LL (2005) Structure of a cellulose degrading bacterial community during anaerobic digestion. Biotechnol Bioeng 92:871–878
O’Connell D (2006) Industrial microbiology: ‘microdiesel’ to the rescue? Nat Rev Microbiol 4:723
Odling-Smee L (2007) Biofuels bandwagon hits a rut. Nature 446:483
Ohta K, Beall DS, Mija JP, Shanmugam KT, Ingram LO (1991) Genetic improvement of Escherichia coli for ethanol production: chromosomal integration of Zymomonas mobilis genes encoding pyruvate decarboxylase and alcohol dehydrogenase II. Appl Environm Microbiol 57:893–900
Pesta G, Meyer-Pittroff R, Russ W (2006) Utilization of whey. In: Oreopoulou V, Russ W (eds) Utilization of byproducts and treatment of waste in the food industry. Springer, New York, pp 1–11, (1)
RFA (2007) Renewable Fuels Association: Statistics. Washington DC, online 15.05.2007: http://www.ethanolrfa.org/industry/statistics/
Sanderson K (2006) A field in ferment. Nature 444:673–676
Schwarz WH, Gapes JR (2006) Butanol—rediscovering a renewable fuel. BioWorld Europe 01-2006, pp 16–19
Schwarz WH, Gapes JR, Zverlov VV, Antoni D, Erhard W, Slattery M (2006) Personal communication and demonstration at the TU Muenchen (Campus Garching and Weihenstephan) in June 2006
Schwarz WH, Slattery M, Gapes JR (2007) The ABC of ABE. BioWorld Europe 02-2007, pp 8–10
Siso MIG (1996) The biotechnological utilization of cheese whey: a review. Bioresour Technol 57:1–11
Stern N (2006) The economics of climate change. The Stern Review. Cabinet Office – HM Treasury. Cambridge University Press http://www.hm-treasury.gov.uk/independent_reviews/stern_review_economics_climate_change/stern_review_report.cfm
Syutsubo K, Nagaya Y, Sakai S, Miya A (2005) Behavior of cellulose-degrading bacteria in thermophilic anaerobic digestion process. Water Sci Technol 52:79–84
Tailliez P, Girard H, Millet J, Beguin P (1989) Enhanced cellulose fermentation by an asporogenous and ethanol-tolerant mutant of Clostridium thermocellum. Appl Environ Microbiol 55:207–211
Torney F, Noeller L, Scarpa A, Wang K (2007) Genetic engineering approaches to improve bioethanol production from maize. Curr Opin Biotechnol 18:1–7
Wang Q (2006) Biomethanol conversion from sugar beet pulp with pectin methyl esterase. Master thesis, University of Maryland, https://drum.umd.edu/dspace/bitstream/1903/3833/1/umi-umd-3678.pdf
Weuster-Botz D (1993) Continuous ethanol production by Zymomonas mobilis in a fluidized bed reactor. I: Kinetic studies of immobilization in macroporous glass beads. Appl Microbiol Biotechnol 39:679–684
Wu SY, Hung CH, Lin CN, Chen HW, Lee AS, Chang JS (2005) Fermentative hydrogen production and bacterial community structure in high-rate anaerobic bioreactors containing silicone-immobilized and self-flocculated sludge. Biotechnol Bioeng 93:934–946
Yadvika, Santosh S, Sreekrishnan TR, Kohli S, Rana V (2004) Enhancement of biogas production from solid substrates using different techniques—a review. Bioresour Technol 95:1–10
Yusuf C (2007) Biodiesel from microalgae. Biotechnol Adv 3:294–306
Zaborsky OR (1982) Chemicals from renewable resources: an endorsement for biotechnology. Enzyme Microbiol Technol 4:364–365
Zaldivar J, Nielsen J, Olsson L (2001) Fuel ethanol production from lignocellulose: a challenge for metabolic engineering and process integration. Appl Microbiol Biotechnol 56:17–34
Zeikus JG (1980) Chemical and fuel production by anaerobic bacteria. Annu Rev Microbiol 34:423–464
Zhou S, Davis FC, Ingram LO (2001) Gene integration and expression and extracellular secretion of Erwinia chrysanthemi endoglucanase CelY (celY) and CelZ (celZ) in ethanologenic Klebsiella oxytoca P2. Appl Environ Microbiol 67:6–14
Zverlov VV, Berezina O, Velikodvorskaya GA, Schwarz WH (2006) Bacterial acetone and butanol production by industrial fermentation in the Soviet Union: use of hydrolyzed agricultural waste for biorefinery. Appl Microbiol Biotechnol 71:587–597
Acknowledgement
We thank the German Research Foundation (DFG) and the German Agency of Renewable Resources (FNR) for support to WHS and VVZ. The advice and support of M. Slattery, J. R. Gapes, W. Hiegl, R. Igelspacher and A. Schwarz in preparing, reading and commenting on the text is gratefully acknowledged.
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Antoni, D., Zverlov, V.V. & Schwarz, W.H. Biofuels from microbes. Appl Microbiol Biotechnol 77, 23–35 (2007). https://doi.org/10.1007/s00253-007-1163-x
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DOI: https://doi.org/10.1007/s00253-007-1163-x