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NADH- vs NADPH-coupled reduction of 5-hydroxymethyl furfural (HMF) and its implications on product distribution in Saccharomyces cerevisiae

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Abstract

Saccharomyces cerevisiae alcohol dehydrogenases responsible for NADH-, and NADPH-specific reduction of the furaldehydes 5-hydroxymethyl-furfural (HMF) and furfural have previously been identified. In the present study, strains overexpressing the corresponding genes (mut-ADH1 and ADH6), together with a control strain, were compared in defined medium for anaerobic fermentation of glucose in the presence and absence of HMF. All strains showed a similar fermentation pattern in the absence of HMF. In the presence of HMF, the strain overexpressing ADH6 showed the highest HMF reduction rate and the highest specific ethanol productivity, followed by the strain overexpressing mut-ADH1. This correlated with in vitro HMF reduction capacity observed in the ADH6 overexpressing strain. Acetate and glycerol yields per biomass increased considerably in the ADH6 strain. In the other two strains, only the overall acetate yield per biomass was affected. When compared in batch fermentation of spruce hydrolysate, strains overexpressing ADH6 and mut-ADH1 had five times higher HMF uptake rate than the control strain and improved specific ethanol productivity. Overall, our results demonstrate that (1) the cofactor usage in the HMF reduction affects the product distribution, and (2) increased HMF reduction activity results in increased specific ethanol productivity in defined mineral medium and in spruce hydrolysate.

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Acknowledgments

This work was sponsored by the Swedish Energy Agency and the Swedish Foundation for International Cooperation in Research and Higher Education. We would like to thank Prof. Bärbel Hahn-Hägerdal for critically reading the manuscript.

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Author notes

  1. Anja Röder

    Present address: Institut für Medizinische Mikrobiologie, Virologie und Hygiene, Universitätsklinikum Hamburg-Eppendorf, Campus Forschung, N27, 2 OG Martinistraβe, 52 20246, Hamburg, Germany

Authors and Affiliations

  1. Department of Applied Microbiology, Lund University, P.O. Box 124, 221 00, Lund, Sweden

    João R. M. Almeida, Anja Röder, Boaz Laadan & Marie-F. Gorwa-Grauslund

  2. Department of Chemical Engineering, Lund University, P.O. Box 124, 221 00, Lund, Sweden

    Tobias Modig & Gunnar Lidén

Authors
  1. João R. M. Almeida
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  6. Marie-F. Gorwa-Grauslund
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Correspondence to Marie-F. Gorwa-Grauslund.

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Almeida, J.R.M., Röder, A., Modig, T. et al. NADH- vs NADPH-coupled reduction of 5-hydroxymethyl furfural (HMF) and its implications on product distribution in Saccharomyces cerevisiae . Appl Microbiol Biotechnol 78, 939–945 (2008). https://doi.org/10.1007/s00253-008-1364-y

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