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Purification and characterization of plantaricin LR14: a novel bacteriocin produced by Lactobacillus plantarum LR/14

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Abstract

Bacteriocin produced by Lactobacillus plantarum strain LR/14 was purified to homogeneity by a multi-step protocol consisting of ammonium sulfate precipitation, cation-exchange chromatography, gel-filtration, and reverse-phase FPLC. L. plantarum LR/14 secreted a low-molecular-weight bacteriocin consisting of two peptides designated as plantaricin LR14α and -β with molecular mass of 3,012.46 and 5,605.74 Da, respectively. The purified peptides were characterized to be highly thermostable and active in acidic pH range, with a pI of >10.0. Both α and β peptides showed bactericidal mode of action against indicator strain, Micrococcus luteus and together showed a synergistic action. These peptides were differentially sensitive to a range of proteolytic enzymes, indicating differences in their composition. Amino acid sequencing revealed that the N-terminus in both the cases is blocked; thus, only a partial sequence could be obtained after CNBr digestion. These sequences, when compared with those available in the database, showed no homology with known bacteriocins, indicating it to be a novel compound.

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Acknowledgements

This work was supported by grants from Council of Scientific and Industrial Research (CSIR) and Department of Biotechnology (DBT), India. The authors thank Dr. A.K. Panda, National Institute of Immunology, New Delhi for his technical advice and helpful discussion. The facilities provided to the Department of Genetics, by University Grants Commission under SAP and by Department of Science and Technology, Government of India under FIST programs are thankfully acknowledged. SKT was supported by an ICMR fellowship.

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  1. Department of Genetics, University of Delhi South Campus, New Delhi, 110021, India

    Santosh Kumar Tiwari & Sheela Srivastava

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  1. Santosh Kumar Tiwari
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  2. Sheela Srivastava
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Correspondence to Sheela Srivastava.

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Tiwari, S.K., Srivastava, S. Purification and characterization of plantaricin LR14: a novel bacteriocin produced by Lactobacillus plantarum LR/14. Appl Microbiol Biotechnol 79, 759–767 (2008). https://doi.org/10.1007/s00253-008-1482-6

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  • DOI: https://doi.org/10.1007/s00253-008-1482-6

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