Abstract
Xylanases have received great attention in the development of environment-friendly technologies in the paper and pulp industry. Their use could greatly improve the overall lignocellulosic materials for the generation of liquid fuels and chemicals. Fungi are widely used as xylanase producers and are generally considered as more potent producers of xylanases than bacteria and yeasts. Large-scale production of xylanases is facilitated with the advent of genetic engineering. Recent breakthroughs in genomics have helped to overcome the problems such as limited enzyme availability, substrate scope, and operational stability. Genes encoding xylanases have been cloned in homologous and heterologous hosts with the objectives of overproducing the enzyme and altering its properties to suit commercial applications. Owing to the industrial importance of xylanases, a significant number of studies are reported on cloning and expression of the enzymes during the last few years. We, therefore, have reviewed recent knowledge regarding cloning of fungal xylanase genes into various hosts for heterologous production. This will bring an insight into the current status of cloning and expression of the fungal xylanases for industrial applications.
Similar content being viewed by others
References
Ahmed S, Aslam N, Latif F, Rajoka MI, Jamil A (2005) Molecular cloning of cellulase genes from Trichoderma harzianum. In: Atta-ur-Rehman, Choudhary, Khan (eds) Frontiers in natural product chemistry, vol 1. Bentham Science, The Netherlands, pp 73–75
Ahmed S, Jabeen A, Jamil A (2007) Xylanase from Trichoderma harzianum: enzyme characterization and gene isolation. J Chem Soc Pak 29:176–182
Alcalde M, Ferrer M, Plou FJ, Ballesteros A (2006) Environmental biocatalysts: from remediation with enzymes to novel green processes. Trends Biotechnol 24:281–287
Apel P, Panaccione D, Holden F, Walton J (1993) Cloning and targeted gene disruption of XYL1 a β-1,4-xylanase gene from the maize pathogen Cochliobolus carbonum. Mol Plant–Microb Interact 6:467–473
Apel-Birkhold P, Walton J (1996) Cloning, disruption and expression of two endo-β-1,4-xylanase genes, XYL2 and XYL3, from Cochliobolus carbonum. Appl Environ Microbiol 62:4129–4135
Baba T, Shinke R, Nanmori T (1994) Identification and characterization of clustered genes for thermostable xylan-degrading enzymes, β-xylosidase and xylanase of Bacillus stearothermophilus 21. Appl Environ Microbiol 60:2252–2258
Badhan AK, Chadha BS, Kaur J, Saini HS, Bhat MK (2007) Production of multiple xylanolytic and cellulolytic enzymes by thermophilic fungus Myceliophthora sp. IMI 387099. Bioresour Technol 98:504–510
Balaa AB, Wouters J, Dogne S, Rossini C, Schaus J, Depiereux E, Vandenhaute J, Housen I (2006) Identification, cloning, and expression of Scytalidium acidophilum XYLI gene encoding for acidophilic xylanase. Biosci Biotechnol Biochem 70:269–272
Baneyx F (1999) Recombinant protein expression in Escherichia coli. Curr Opin Biotechnol 10:411–421
Basaran P, Hang YD, Basaran N, Worobo RW (2001) Cloning and heterologous expression of xylanase from Pichia stipitis in Escherichia coli. J Appl Microbiol 90:248–255
Beg QK, Kapoor M, Mahajan L, Hoondal GS (2001) Microbial xylanases and their industrial applications: a review. Appl Microbiol Biotechnol 56:326–338
Bergquist P, Teo'O V, Gibb M (2002) Expression of xylanase enzymes from thermophilic microorganisms in fungal host. Extremophiles 6:177–184
Berrin JG, Wiliamson G, Puigserver A, Chaix JC, McLauchlan WR, Juge N (2000) High level production of recombinant fungal endo-β-1,4-xylanase in the methylotrophic yeast Pichia pastoris. Protein Expr Purif 19:179–187
Biely P (1985) Microbial xylanolytic systems. Trends Biotechnol 3:286–290
Boonyapakron K, Pootanakit K, Chantasingh D, Kirtikara K, Eurwilaichitr L (2005) Cloning and expression of xylanase 10 from Cryptovalsa mangrovei (BCC7197) in Pichia pastoris. DNA Seq 16:372–378
Buchert J, Tenkanen M, Kantelinen A, Viikari L (1994) Application of xylanases in the pulp and paper industry. Bioresour Technol 50:65–72
Cao Y, Qiao J, Li Y, Lu W (2007) De novo synthesis, constitutive expression of Aspergillus sulphureus beta-xylanase gene in Pichia pastoris and partial enzymic characterization. Appl Microbiol Biotechnol 76:579–585
Cereghino JL, Cregg JM (2000) Heterologous protein expression in methylotrophic yeast Pichia pastoris. FEMS Microbiol Rev 24:45–66
Chand S, Mishra P (2003) Research and application of microbial enzymes-India's contribution. Adv Biochem Eng Biotechnol 85:95–124
Chantasingh DK, Champreda PV, Kanokratana P, Eurwilaichitr L (2006) Cloning, expression and characterization of a xylanase 10 from Aspergillus terrus (BCC129) in Pichia pastoris. Protein Expr Purif 46:143–149
Chavez R, Fierro F, Gordillo F, Martín JF, Eyzaguirre J (2001) Electrophoretic karyotype of the filamentous fungus Penicillium purpurogenum and chromosomal location of several xylanolytic genes. FEMS Microbiol Lett 205:379–383
Chavez R, Navarro C, Calderon I, Peorano A, Bull P, Eyzaguirre J (2002) Secretion of endoxylanase A from Penicillium purpurogenum by Saccharomyces cerevisiae transformed with genomic fungal DNA. FEMS Microbiol Lett 212:237–241
Chen H, Li XL, Ljungdahl LG (1997) Sequencing of a 1,3-1,4-beta-d-glucanase (lichenase) from the anaerobic fungus Orpinomyces strain PC-2: properties of the enzyme expressed in Escherichia coli and evidence that the gene has a bacterial origin. J Bacteriol 179:6028–6034
Cheng YF, Yang CH, Liu WH (2005) Cloning and expression of Thermoibifida xylanase gene in the methylotrophic yeast Pichia pastoris. Enzyme Microb Technol 37:541–546
Choi ID, Kim HY, Choi YJ (2000) Gene cloning and characterization of α-glucuronidase of Bacillus stearothermophilus no. 263. Biosci Biotechnol Biochem 64:2530–2537
Collins T, Gerday C, Feller G (2005) Xylanases, xylanase families and extremophilic xylanases. FEMS Microbiol Rev 29:3–23
Cregg JM (1999) Expression in methylotrophic yeast Pichia pastoris. In: Fernandez JM, Hoeffler JP (eds) Gene expression systems. Academic, New York, pp 157–191
Cregg JM, Madden KR, Barringer KJ, Thill GP, Stillman CA (1989) Functional characterization of the two alcohol oxidase genes from the yeast Pichia pastoris. Mol Cell Biol 9:1316–1323
Crous JM, Pretorius IS, van Zyl WH (1995) Cloning and expression of an Aspergillus kawachii endo-1,4-β-xylanase gene in Saccharomyces cerevisiae. Curr Genet 28:467–473
Dalboge H (1997) Expression cloning of fungal enzyme genes; a novel approach for efficient isolation of enzyme genes of industrial relevance. FEMS Microbiol Rev 21:29–42
Damaso MCT, Almeida MS, Kurtenbach E, Martins OB, Pereira N, Andrade CMMMC, Albano RM (2003) Optimized expression of a thermostable xylanase from Thermomyces lanuginosus in Pichia pastoris. Appl Environ Microbiol 69:6064–6072
Das RC, Shultz JL (1987) Secretion of heterologous proteins from Saccharomyces cerevisiae. Biotechnol Prog 3:43–48
de Faria FP, Teo VSJ, Bergquist PL, Azevedo MO, Nevalainen KM (2002) Expression and processing of major xylanase (XYN2) from the thermophilic fungus Humicola grisea var. thermoidea in Trichoderma reesei. Lett Appl Microbiol 34:119–123
Decelle B, Tsang A, Storms RK (2004) Cloning, functional expression and characterization of three Phanerochaete chyrsosporium endo-1,4-β-xylanases. Curr Genet 46:166–175
Degefu Y, Lohtander K, Paulin L (2004) Expression patterns and phylogenetic analysis of two xylanase genes (htxyl1 and htxyl2) from Helminthosporium turcicum, the cause of northern leaf blight of maize. Biochimie 86:83–90
Den Haan R, van Zyl WH (2003) Enhanced xylan degradation and utilization by Pichia stipitis overproducing fungal xylanolytic enzymes. Enzyme Microb Technol 33:620–628
Deng P, Li D, Cao Y, Lu W, Wang C (2006) Cloning of a gene encoding an acidophilic endo-β-1,4-xylanase obtained from Aspergillus niger CGMCC1067 and constitutive expression in Pichia pastoris. Enzyme Microb Technol 39:1096–1102
Diaz R, Sapag A, Peirano A, Steiner J, Eyzaguirre J (1997) Cloning, sequencing and expression of the cDNA of endoxylanase B from Penicillium purpurogenum. Gene 187:247–251
Ebanks R, Dupont M, Shareck F, Morosoli R, Kluepfel D, Dupont C (2000) Development of an Escherichia coli expression system and thermostability screening assay for libraries of mutant xylanase. J Ind Microbiol Biotech 25:310–314
Egli T, van Dijken JP, Veenhuis M, Harder W, Fiechter A (1980) Methanol metabolism in yeast: regulation of the synthesis of catabolic enzymes. Arch Microbiol 124:115–121
Emami K, Hack E (1998) A xylanase gene from Cochliobolus sativus (Bipolaris sorokiniana). In: Duveiller E, Dubin HJ, Reeves J, McNab A (eds) Helminthsosprium blights of wheat: spot blotch and tan spot. CIMMYT, Mexico, pp 314–321
Emami K, Hack E (2001) Characterization of a xylanase gene from Cochliobolus sativus and its expression. Mycol Res 105:352–359
Fang H-Y, Chang S-M, Lan C-H, Fang TJ (2008) Purification and characterization of a xylanase from Aspergillus carneus M34 and its potential use in photoprotectant preparation. Process Biochem 43:49–55
Fengxia L, Mei L, Zhaoxin L, Xiaomei B, Haizhen Z, Yi W (2008) Purification and characterization of xylanase from Aspergillus ficuum AF-98. Bioresour Technol 99:5938–5941
Gellissen G (2000) Heterologous protein production in methylotrophic yeasts. Appl Microbiol Biotechnol 54:741–750
Giesbert S, Lepping HB, Tenberge KB, Tudzynski P (1998) The xylanolytic system of Claviceps purpurea: cytological evidence for secretion of xylanases in infected rye tissue and molecular characterization of two xylanase genes. Biochem Cell Biol 88:1020–1030
Han SO, Yukawa H, Inui M, Doi RH (2003) Regulation of expression of cellulosomal cellulase and hemicellulase genes in Clostridium cellulovorans. J Bacteriol 185:6067–6075
Hessing JG, Rotterdam CV, Verbake JM, Roza M, Maat J, Gorcom RFV, Hondel CAVD (1994) Isolation and characterization of a 1,4-β-endoxylanase gene of A. awamori. Curr Genet 26:228–232
Hitzeman RA, Hagie FE, Hayflick JS, Chen CY, Seeburg PH, Derynck R (1982) The primary structure of the Saccharomyces cerevisiae gene for 3-phosphoglycerate kinase. Nucleic Acids Res 10:7791–7808
Huang YH, Huang CT, Hseu RS (2005) Effects of dockerin domains on Neocallimastix frontalis xylanases. FEMS Microbiol Lett 243:455–460
Ilmen M, Thrane C, Penttila M (1996) The glucose repressor gene cre1 of Trichoderma: isolation and expression of a full-length and a truncated mutant form. Mol Gen Genet 251:451–460
Ilmen M, Saloheimo A, Onnela ML, Pentilla ME (1997) Regulation of cellulase gene expression in the filamentous fungus T. reesei. Appl Environ Microbiol 63:1298–1306
Innis MA, Holland MJ, McCabe PC, Cole GE, Wittman VP, Tal R, Watt KWK, Gelfand DH, Holland JP, Meade JH (1985) Expression, glycosylation, and secretion of an Aspergillus glucoamylase by Saccharomyces cerevisiae. Science 228:21–26
Ito K, Ikemasu T, Ishikawa T (1992) Cloning and sequencing of the xynA gene encoding xylanase A of Aspergillus kawachii. Biosci Biotechnol Biochem 56:906–912
Jamil A, Naim S, Ahmed S, Ashraf M (2005) Production of industrially important enzymes using molecular approaches; cellulases and xylanases. In: Thangadurai D, Pullaiah T, Balatti PA (eds) Genetic resources and biotechnology II, volume two. Regency, New Delhi
Jeffries T (1996) Biochemistry and genetics of microbial xylanases. Curr Opin Biotechnol 7:337–342
Jun H, Bing Y, Zhang K, Ding X, Daiwen C (2008) Expression of a Trichoderma reesei β-xylanase gene in Escherichia coli and activity of the enzyme on fiber-bound substrates. Protein Expr Purif 67:1–6
Karlsson EN, Dahlberg L, Torto N, Gorton L, Holst O (1998) Enzymatic specificity and hydrolysis pattern of the catalytic domain of the xylanase xyn1 from Rhodothermus marinus. J Biotechnol 60:23–35
Kaur J, Sharma R (2006) Directed evolution: an approach to engineer enzymes. Crit Rev Biotechnol 26:165–199
Khandeparker R, Numan MT (2008) Bifunctional xylanases and their potential use in biotechnology. J Ind Microbiol Biotech 35:635–644
Kimura T, Kitamoto N, Kito Y, Karita S, Sakka K, Ohmiya K (1998) Molecular cloning of xylanase gene xynG1 from Aspergillus oryzae KBN 616, a Shoyu Koji mold, and analysis of its expression. J Ferment Bioeng 85:10–16
Kimura T, Ito J, Kawano A, Makino T, Kondo H, Karita S, Sakka K, Ohmiya K (2000) Purification, characterization, and molecular cloning of acidophilic xylanase from Penicillium sp. 40. Biosci Biotechnol Biochem 64:1230–1237
Kimura T, Suzuki H, Furuhashi H, Aburatani T, Morimoto K, Sakka K, Ohmiya K (2002) Molecular cloning, characterization and expression analysis of the xynF3 gene from Aspergillus oryzae. Biosci Biotechnol Biochem 66:285–292
Kitamoto N, Yoshino S, Ito M, Kimura T, Ohmiya K, Tukagoshi N (1998) Repression of the expression of genes encoding xylanolytic enzymes in Aspergillus oryzae by introduction of multiple copies of the xynF1 promoter. Appl Microbiol Biotechnol 50:558–563
Kitamoto N, Yoshino S, Ohmiya K, Tsukagoshi N (1999) Purification and characterization of the overexpressed Aspergillus oryzae xylanase, xynF1. Biosci Biotechnol Biochem 63:1791–1794
Korona B, Korona D, Bielecki S (2006) Efficient expression and secretion of two co-produced xylanases from Aspergillus niger in Pichia pastoris directed by their native signal peptides and the Saccharomyces cerevisiae α-mating factor. Enzyme Microb Technol 39:683–689
Krisana A, Rutchadaporn S, Jarupan G, Lily E, Sutipa T, Kanyawim K (2005) Endo β-1,4-xylanase B from Aspergillus cf. niger BCC14405 isolated in Thailand: purification, characterization and gene isolation. J Biochem Mol Biol 38:17–23
Kulkarni N, Shendye A, Rao M (1999) Molecular and biotechnological aspects of xylanases. FEMS Microbiol Rev 23:411–456
Kumar R, Singh S, Sing OV (2008) Bioconversion of lignocellulosic biomass: biochemical and molecular prospectives. J Ind Microbiol Biotech 33:377–391
Kurjan J, Herskowitz I (1982) Structure of a yeast pheromone gene (MF alpha): a putative alpha-factor precursor contains four tandem copies of mature alpha-factor. Cell 30:933–943
La Grange DC, Pretorius IS, van Zyl WH (1996) Expression of a Trichoderma reesei β-xylanase gene (xyn2) in Saccharomyces cerevisiae. Appl Environ Microbiol 62:1036–1044
La Grange DC, Claeyssens M, Pretorius IS, van Zyl WH (2000) Co-expression of the Bacillus pumilus β-xylosidase (xynB) gene with the Trichoderma reesei β-xylosidase 2 (xyn2) gene in yeast Saccharomyces cerevisiae. Appl Microbiol Biotechnol 54:195–200
La Grange DC, Pretorius IS, Claeyssens M, van Zyl WH (2001) Degradation of xylan to d-xylose by recombinant Saccharomyces cerevisiae coexpressing the Aspergillus niger beta-xylosidase (xlnD) and the Trichoderma reesei xylanase II (xyn2) genes. Appl Environ Microbiol 67:5512–5519
Lee CC, Wong DW, Robertson GH (2005) Cloning and characterization of the xyn11A gene from Lentinula edodes. Protein J 24:21–26
Levasseur A, Asther M, Record E (2005) Overproduction and characterization of xylanase B of Aspergillus niger. Can J Microbiol 51:177–183
Li XL, Ljungdahl LG (1994) Cloning, sequencing and regulation of a xylanase gene from Aureobasidium pullulans Y-2311-1. Appl Environ Microbiol 60:3160–3166
Li XL, Ljungdahl LG (1996) Expression of Aureobasidium pullulans xynA in, and secretion of the xylanase from, Saccharomyces cerevisiae. Appl Environ Microbiol 62:209–213
Li XL, Skory CD, Ximenes EA, Jordan DB, Dien BS, Hughes SR, Cotta MA (2007) Expression of an AT-rich xylanase gene from the anaerobic fungus Orpinomyces sp. strain PC-2 in and secretion of the heterologous enzyme by Hypocrea jecorina. Appl Microbiol Biotechnol 74:1264–1275
Liu MQ, Wang XY, Sun JY (2006) Expression of recombinant Aspergillus niger xylanase A in Pichia pastoris and its action on xylan. Protein Expr Purif 48:292–299
Liu JR, Chung-Hang D, Xin Z, Jason T, Kuo-Joan C, Cheng-Kang P (2008) Cloning of a rumen fungal xylanase gene and purification of the recombinant enzyme via artificial oil bodies. Appl Microbiol Biotechnol 79:225–233
Luttig M, Pretorius IS, van Zyl WH (1997) Cloning of two β-xylanase encoding genes from Aspergillus niger and their expression in Saccharomyces cerevisiae. Biotechnol Lett 19:411–415
Mach RL, Zeilinger S (2003) Regulation of gene expression in industrial fungi: Trichoderma. Appl Microbiol Biotechnol 60:515–522
Mantyla A, Paloheimo M, Suominen P (1998) Industrial mutants and recombinants strains of Trichoderma reesei. In: Harman GE, Kubicek CP (eds) Trichoderma and Gliocladium, vol 2. Taylor and Francis, London, pp 291–309
Mantyla A, Paloheimo M, Hakola S, Lindberg E, Leskinen S, Kallio J, Vehmaanpera J, Lantoo R, Suominen P (2007) Production in Trichoderma reesei of three xylanases from Chaetomium thermophile: a recombinant thermoxylanase for bleaching of kraft pulp. Appl Microbiol Biotechnol 76:377–386
Mergulhaoa FJM, Summersb DK, Monteiro GA (2005) Recombinant protein secretion in Escherichia coli. Biotechnol Adv 23:177–202
Moreau A, Durand S, Morosoli R (1992) Secretion of a Cryptococcus albidus xylanase in Saccharomyces cerevisiae. Gene 116:109–113
Moure A, Gullón P, Domínguez H, Parajo JC (2006) Advances in the manufacture, purification and applications of xylo-oligosaccharides as food additives and utraceuticals. Process Biochem 41:1913–1923
Muller S, Sandal T, Kamp-Hansen P, Dalboge H (1998) Comparison of expression systems in the yeasts Saccharomyces cerevisiae, Hansenula polymorpha, Klyveromyces lactis, Schizosaccharomyces pombe and Yarrowia lipolytica. Cloning of two novel promoters from Yarrowia lipolytica. Yeast 14:1267–1283
Nair SG, Sindhu R, Shashidhar S (2008) Purification and biochemical characterization of two xylanases from Aspergillus sydowii SBS 45. Appl Biochem Biotechnol 149:229–243
Nevalainen KMH, Teo VSJ, Bergquist PL (2005) Heterologous protein expression in filamentous fungi. Trends Biotechnol 23:468–474
Ninawe S, Kapoor M, Kuhad RC (2008) Purification and characterization of extracellular xylanase from Streptomyces cyaneus SN32. Bioresour Technol 99:1252–1258
Ning L, Yang P, Wang Y, Luo H, Meng K, Wu N, Fan Y, Yao B (2008) Cloning, expression, and characterization of protease-resistant xylanase from Streptomyces fradiae var. k11. J Microbiol Biotechnol 18:410–416
Ogasawara W, Shida Y, Furukawa T, Shimada R, Nakagawa S, Kawamura M, Yagyu T, Kosuge A, Xu J, Nogawa M, Okada H, Morikawa Y (2006) Cloning, functional expression and promoter analysis of xylanase III gene from Trichoderma reesei. Appl Microbiol Biotechnol 72:995–1003
Ohta K, Moriyama S, Tanaka H, Shige T, Akimoto H (2001) Purification and characterization of an acidophilic xylanase from Aureobasidium pullulans var. melanigenum and sequence analysis of the encoding gene. J Biosci Bioeng 92:262–270
Okada H, Wakamatsu M, Takano Y, Nogawa M, Morikawa Y (1999) Expression of two Trichoderma reesei xylanases in the fission yeast Schizosaccaromyces pombe. J Biosci Bioeng 88:563–566
Parachin NS, Siqueira S, de Faria FP, Torres FAG, de Moraes LMP (2009) Xylanases from Cryptococcus flavus isolate I-11: enzymatic profile, isolation and heterologous expression of CfXYN1 in Saccharomyces cerevisiae. J Mol Catal B Enzym 59:52–57
Pedersen M, Lauritzen HK, Frisvad JC, Meyer AS (2007) Identification of thermostable beta-xylosidase activities produced by Aspergillus brasiliensis and Aspergillus niger. Biotechnol Lett 29:743–748
Perez-Gonzalez AJ, De Graff LH, Visser J, Ranmon D (1996) Molecular cloning and expression in Saccharomyces cerevisiae of two Aspergillus nidulans xylanase genes. Appl Environ Microbiol 62:2179–2182
Perez-Gonzalez JA, van Peij NNME, Bezoen A, MacCabe AP, Ramon D, Graff LHD (1998) Molecular cloning and transcriptional regulation of the Aspergillus nidulans xlnD gene encoding a β-xylosidase. Appl Environ Microbiol 64:1412–1419
Polizeli ML, Rizzatti AC, Monti R, Terenzi HF, Jorge JA, Amorim DS (2005) Xylanases from fungi: properties and industrial applications. Minireview. Appl Microbiol Biotechnol 67:577–591
Prade RA (1996) Xylanases: from biology to biotechnology. Biotechnol Genet Eng Rev 13:101–131
Rauscher R, Würleitner E, Wacenovsky C, Aro N, Stricker AR, Zeilinger S, Kubicek CP, Penttilä M, Mach RL (2006) Transcriptional regulation of xyn1, encoding xylanase I, in Hypocrea jecorina. Eukaryot Cell 3:447–456
Romanos MA, Scorer CA, Clare JJ (1992) Foreign gene expression in yeast: a review. Yeast 8:423–488
Rose SH, van Zyl WH (2002) Constitutive expression of the Trichoderma reesei beta-1,4-xylanase gene (xyn2) and the beta-1,4-endoglucanase gene (egl) in Aspergillus niger in molasses and defined glucose media. Appl Microbiol Biotechnol 58:461–468
Ruanglek V, Sriprang R, Ratanaphan N, Tirawongsaroj P, Chantasigh D, Tanapongpipat S, Pootanakit K, Eurwilaichitr L (2007) Cloning expression, characterization and high cell density production of recombinant endo 1,4-β-xylanases from Aspergillus niger in Pichias pastoris. Enzyme Microb Technol 41:19–25
Saadia M, Ahmed S, Jamil A (2008) Isolation and cloning of cre1 gene from a filamentous fungus Trichoderma harzianum. Pak J Bot 40:421–426
Saarelainen R, Paloheimo M, Fagerstrom R, Suominen PL, Nevalainen KMH (1993) Cloning, sequencing and enhanced expression of the Trichoderma reesei endoxylanse II (pI 9) gene xln2. Mol Gen Genet 241:497–503
Saleem F, Ahmed S, Jamil A (2008) Isolation of a xylan degrading gene from genomic DNA library of a thermophilic fungus Chaetomium thermophile ATCC 28076. Pak J Bot 40:1225–1230
Salles BC, Teo VSJ, Gibbs MD, Bergquist PL, Filho EXF, Ximenes EA, Nevalainen KM (2007) Identification of two novel xylanase-encoding genes (xyn5 and xyn6) from Acrophialophra nainiana and hetrologous expression of xyn6 in Trichoderma reesei. Biotechnol Lett 29:1195–1201
Sa-Pereira P, Paveia H, Costa-Ferreira M, Aires-Barros MR (2003) A new look at xylanases: an overview of purification strategies. Mol Biotechnol 24:257–281
Schlacher A, Holzmann K, Hayn M, Steiner W, Schwab H (1996) Cloning and characterization of the gene for the thermostable xylanase XynA from Thermomyces lanuginosus. J Biotechnol 49:211–218
Sorensen HP, Mortensen KK (2005) Advanced genetic strategies for recombinant protein expression in Escherichia coli. J Biotechnol 115:113–128
Studier FW, Moffatt BA (1986) Use of bacteriphage T7 RNA polymerase to direct selective high level expression of cloned genes. J Mol Biol 189:113–130
Sun YJ, Liu MQ, Wang XY, Qian LC, Gu SH (2007) Expression of recombinant Thermomonospora fusca xylanase A in Pichia pastoris and xylooligosachharides released from xylan by it. Food Chem 104:1055–1064
Sunna A, Antranikian G (1997) Xylanolytic enzymes from fungi and bacteria. Crit Rev Biotechnol 17:39–67
Tanaka H, Okuno T, Moriyama S, Muguruma M, Ohta K (2004) Acidophilic xylanase from Aureobasidium pullulans: efficient expression and secretion in Pichia pastoris and mutational analysis. J Biosci Bioeng 98:338–343
Tanaka H, Nakamura T, Ohta K (2005) Purification and properties of an extracellular xylanase from Penicillium citrinum and characterization of the encoding gene. J Biosci Bioeng 6:623–630
Tanaka H, Muguruma M, Ohta K (2006) Purification and properties of a family-10 xylanase from Aureobasidium pullulans ATCC 20524 and characterization of the encoding gene. Appl Microbiol Biotechnol 70:202–211
Teather RM, Wood PJ (1982) Use of Congo red polysaccharides interactions in enumeration and characterization of cellulolytic bacteria form the bovine rumen. Appl Environ Microbiol 43:777–780
Tsai CT, Huang CT (2008) Overexpression of the Neocallimastix frontalis xylanase gene in the methylotrophic yeasts Pichia pastoris and Pichia methanolica. Enzyme Microb Technol 42:459–465
van Peij NN, Visser J, De Graaff LH (1998) Isolation and analysis of xlnR, encoding a transcriptional activator co-ordinating xylanolytic expression in Aspergillus niger. Mol Microbiol 27:131–142
Wakiyama M, Tanaka H, Yoshihara K, Hayashi S, Ohta K (2008) Purification and properties of family-10 endo-1,4-β-xylanase from Penicillium citrinum and structural organization of encoding gene. J Biosci Bioeng 105:367–374
Wong KKY, Tan LUL, Saddler JN (1988) Multiplicity of β-1,4-xylanase in microorganisms: functions and applications. Microbiol Rev 52:305–317
Wu SC, Halley JE, Luttig C, Fernekes LM, Sanchez GG, Dravill AG, Albersheim P (2006) Identification of an endo-β-1,4-d-xylanase from Magnaporhte grisea by gene knockout analysis, purification, and heterologous expression. Appl Environ Microbiol 72:986–993
Xue GP, Denman SE, Glassop D, Johnson JS, Dierens LM, Gobius KS, Aylward JH (1995) Modification of a xylanase cDNA isolated from an anaerobic fungus Neocallimastix patriciarum for high-level expression in Escherichia coli. J Biotechnol 38:269–277
Yoshino S, Oishi M, Moriyama R, Kato M, Tsukagoshi N (1995) Two family G xylanase genes from Chaetomium gracile and their expression in Aspergillus nidulans. Curr Genet 29:73–80
Youderian P, Bouvier S, Susskind MM (1982) Sequence determinants of promoter activity. Cell 30:843–853
Yu EKC, Tan LUL, Chan MK-H, Deschatelets L, Saddler JN (1987) Production of thermostable xylanase by a thermophilic fungus, Thermoascus aurantiacus. Enzyme Microb Technol 9:16–24
Zhang GM, Huang J, Huang GR, Ma LX, Zhang XE (2007) Molecular cloning and expression of a new xylanase gene from Plectosphaerella cucmerina. Appl Microbiol Biotechnol 74:339–346
Zhou C, Bai J, Deng S, Wang J, Zhu J, Wu M, Wang W (2008) Cloning of a xylanase gene from Aspergillus usamii and its expression in Escherichia coli. Bioresour Technol 99:831–838
Acknowledgement
The authors highly acknowledge the Higher Education Commission, Government of Pakistan for providing the grant to conduct research work on molecular cloning and expression of fungal xylanases.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Ahmed, S., Riaz, S. & Jamil, A. Molecular cloning of fungal xylanases: an overview. Appl Microbiol Biotechnol 84, 19–35 (2009). https://doi.org/10.1007/s00253-009-2079-4
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00253-009-2079-4