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One-step transformation of the dimorphic yeast Yarrowia lipolytica

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Abstract

An efficient one-step transformation method for the dimorphic yeast Yarrowia lipolytica is described. Using cells grown overnight on agar plates, the whole process is carried out within 1 h. The transformant clones could be recovered on selective plates as early as 36–48 h after plating. The efficiency was better than 105 transformants/μg replicative plasmid DNA. Effects of cell density, dithiothreitol, heat shock, poly(ethylene glycol) 4000 concentration and the wetness of selective plates were investigated.

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Authors and Affiliations

  1. Laboratoire de Genétique Moléculaire et Cellulaire, Institut National de la Recherche Agromique-CNRS, Centre de Biotechnologie Agro-Industrielle, Institut National Agronomique Paris-Grignon, F-78850, Thiverval-Grignon, Paris, France Tel.: (+33 1) 30815443 Fax: (+33 1) 30815457, , , , , , FR

    D.-C. Chen, J.-M. Beckerich & C. Gaillardin

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  1. D.-C. Chen
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  2. J.-M. Beckerich
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  3. C. Gaillardin
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Received: 17 February 1997 / Received revision: 4 April 1997 / Accepted: 19 April 1997

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Chen, DC., Beckerich, JM. & Gaillardin, C. One-step transformation of the dimorphic yeast Yarrowia lipolytica . Appl Microbiol Biotechnol 48, 232–235 (1997). https://doi.org/10.1007/s002530051043

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  • DOI: https://doi.org/10.1007/s002530051043

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