Abstract
An efficient one-step transformation method for the dimorphic yeast Yarrowia lipolytica is described. Using cells grown overnight on agar plates, the whole process is carried out within 1 h. The transformant clones could be recovered on selective plates as early as 36–48 h after plating. The efficiency was better than 105 transformants/μg replicative plasmid DNA. Effects of cell density, dithiothreitol, heat shock, poly(ethylene glycol) 4000 concentration and the wetness of selective plates were investigated.
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Received: 17 February 1997 / Received revision: 4 April 1997 / Accepted: 19 April 1997
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Chen, DC., Beckerich, JM. & Gaillardin, C. One-step transformation of the dimorphic yeast Yarrowia lipolytica . Appl Microbiol Biotechnol 48, 232–235 (1997). https://doi.org/10.1007/s002530051043
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DOI: https://doi.org/10.1007/s002530051043