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Monocyte-derived DC maturation strategies and related pathways: a transcriptional view

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Abstract

Ex vivo production of highly stimulator mature dendritic cells (DCs) for cellular therapy has been used to treat different pathological conditions with the aim of inducing a specific immune response. In the last decade, several protocols have been developed to mature monocyte-derived DCs: each one has led to the generation of DCs showing different phenotypes and stimulatory abilities, but it is not yet known which one is the best for inducing effective immune responses. We grouped several different maturation protocols according to the downstream pathways they activated and reviewed the shared features at a transcriptomic level to reveal the potential of DCs matured by each protocol to develop Th-polarized immune responses.

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Acknowledgments

This work is supported by the Intramural Programs of the National Institutes of Health Clinical Center and National Cancer Institute.

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Authors and Affiliations

  1. Cell Processing Section, Department of Transfusion Medicine, Clinical Center, National Institutes of Health, Bethesda, MD, 20892, USA

    Luciano Castiello, Marianna Sabatino, Ping Jin & David F. Stroncek

  2. Laboratory of Cellular and Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD, 20892, USA

    Carol Clayberger & Alan M. Krensky

  3. Infectious Disease and Immunogenetics Section, Department of Transfusion Medicine, Clinical Center, and Center for Human Immunology (CHI), National Institutes of Health, Bethesda, MD, 20892, USA

    Francesco M. Marincola

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  1. Luciano Castiello
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  2. Marianna Sabatino
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  3. Ping Jin
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  4. Carol Clayberger
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  7. David F. Stroncek
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Correspondence to David F. Stroncek.

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Castiello, L., Sabatino, M., Jin, P. et al. Monocyte-derived DC maturation strategies and related pathways: a transcriptional view. Cancer Immunol Immunother 60, 457–466 (2011). https://doi.org/10.1007/s00262-010-0954-6

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