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Continuous Enrichment Culture and Molecular Monitoring to Investigate the Microbial Diversity of Thermophiles Inhabiting Deep-Sea Hydrothermal Ecosystems

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Abstract

The microflora developing during a continuous enrichment culture from a hydrothermal chimney sample was investigated by molecular methods. The culture was performed in a gas-lift bioreactor under anaerobic conditions, at 90°C and pH 6.5, on a complex medium containing sulfur as the terminal electron acceptor. Archaeal and bacterial diversity was studied. Microorganisms affiliated with the genera Pyrococcus, Marinitoga, and Bacillus were detected through DGGE analysis of 16S rDNA. Additional sequences phylogenetically related to Thermococcus and ε-Proteobacteria were detected by cloning and sequencing of 16S rDNA from two samples of the enrichment culture. In comparison, the sequences retrieved from cloning analysis from an enrichment culture performed in a flask (batch condition) using the same culture medium showed that only members of the genus Thermococcus were cultivated. Therefore, continuous enrichment culture using the gas-lift bioreactor can be considered as an efficient and improved method for investigating microbial communities originating from deep-sea hydrothermal vents.

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Acknowledgments

The authors want to thank P.M. Sarradin, chief scientist of the ATOS cruise, as well as the captain and crew of the Atalante and the Victor team. We thank Drs. P. Roger and L. Casalot (IRD) for revising the manuscript. This work was supported by Ifremer, European VENTOX Program, and Région Bretagne.

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Authors and Affiliations

  1. UMR 6197, Laboratoire de Microbiologie des Environnements Extrêmes, IFREMER, Centre de Brest, BP 70, 29280 Plouzané, France

    Anne Postec, Laurent Urios, Françoise Lesongeur, Joël Querellou & Anne Godfroy

  2. Laboratoire de Microbiologie, Institut de Recherche pour le Développement, UR 101, IFR-BAIM, Universités de Provence et de la Méditerranée, ESIL case 925, 163 Avenue de Luminy, 13288, Marseille Cedex 9, France

    Bernard Ollivier

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  1. Anne Postec
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Postec, A., Urios, L., Lesongeur, F. et al. Continuous Enrichment Culture and Molecular Monitoring to Investigate the Microbial Diversity of Thermophiles Inhabiting Deep-Sea Hydrothermal Ecosystems. Curr Microbiol 50, 138–144 (2005). https://doi.org/10.1007/s00284-004-4443-z

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