Abstract
SCO3900, a putative target gene of the developmental regulator WhiI of Streptomyces coelicolor, encodes a protein similar to PadR from Pediococcus pentosaceus. The SCO3900 disruption mutant exhibited a bald phenotype with sparse aerial hyphae. However, a single copy of SCO3900 under the control of its native promoter could not complement the mutant phenotype. A fragment containing SCO3900 and its downstream three genes completely restored the mutant phenotype to the wild-type. These suggested that the disruption of SCO3900 exerted a polar effect on its downstream genes. The co-transcription of SCO3899, SCO3898, and SCO3897 with SCO3900 was confirmed by the RT–PCR. Moreover, overexpression of SCO3900 in the wild-type strain caused the same phenotype as that of the disruption mutant. The results suggested that SCO3900 encodes a negative regulator participating in morphological differentiation of S. coelicolor by influencing the expression of its downstream genes.
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Acknowledgments
We are grateful to Professor Keith Chater (John Innes Centre, Norwich, UK) for providing E. coli ET12567/pUZ8002, pKC1132 and pSET152. We also thank Dr. Brenda Leskiw (University of Alberta, Canada) for the gift of apramycin. This work was supported by grants from the National Natural Science Foundation of China [30670033 to H. T.] and the Ministry of Science and Technology of China [2009CB118905 to H. T.].
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Zhang, G., Tian, Y., Hu, K. et al. SCO3900, Co-Transcripted with Three Downstream Genes, Is Involved in the Differentiation of Streptomyces coelicolor . Curr Microbiol 60, 268–273 (2010). https://doi.org/10.1007/s00284-009-9536-2
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DOI: https://doi.org/10.1007/s00284-009-9536-2