Abstract
A characteristic feature of the mitochondrial expression system in Saccharomyces cerevisiae is the requirement for gene-specific translational activator proteins. Translation of mitochondrial apocytochrome b mRNA requires the nucleus-encoded proteins Cbs1p and Cbs2p. These proteins are thought to tether cytochrome b mRNA to the mitochondrial inner membrane via binding to the 5′ untranslated mRNA leader. Here, we demonstrate by the use of affinity chromatography and coimmunoprecipitation that Cbs2p interacts with the mitoribosomes. We further provide evidence that the C-terminus of Cbs2p is important for ribosome association, while the N-terminal portion is essential for the formation of homomeric structures.
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Acknowledgements
The authors gratefully thank W. Zachariae (MPI-CBG Dresden) for providing plasmid pGA2254, J.M. Herrmann (Universität München), N. Bonnefoy (CNRS, Gif sur Yvette) and A. Chacinska (Universität Freiburg) for the MrpL36, Mrp51p and Nam9p-antibodies, respectively, and K. Ostermann for critical reading of the manuscript. The precious advice of D. Schaft (MPI-CBG Dresden) and C. Hanisch (DKFZ Heidelberg) concerning the TAP method is acknowledged.
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Krause-Buchholz, U., Barth, K., Dombrowski, C. et al. Saccharomyces cerevisiae translational activator Cbs2p is associated with mitochondrial ribosomes. Curr Genet 46, 20–28 (2004). https://doi.org/10.1007/s00294-004-0503-y
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DOI: https://doi.org/10.1007/s00294-004-0503-y