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The complete DNA sequence of the mitochondrial genome of the dermatophyte fungus Epidermophyton floccosum

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Abstract

We report here the complete nucleotide sequence of the 30.9-kb mitochondrial genome of the dermatophyte fungus Epidermophyton floccosum. All genes are encoded on the same DNA strand and include seven subunits of the reduced nicotinamide adenine dinucleotide ubiquinone oxireductase (nad1, nad2, nad3, nad4, nad4L, nad5, and nad6), three subunits of cytochrome oxidase (cox1, cox2, and cox3), apocytochrome b (cob), three subunits of ATP synthase (atp6, atp8, and atp9), the small and large ribosomal RNAs (rns and rnl), and 25 tRNAs. A ribosomal protein gene (rps5) is present as an intronic ORF in the large ribosomal subunit. The genes coding for cob and cox1 carry one intron and nad5 carries two introns with ORFs. The mtDNA of E. floccosum has the same gene order as Trichophyton rubrum mtDNA, with the exception of some tRNA genes. Maximum likelihood phylogenetic analysis confirms T. rubrum as a close relative of E. floccosum. This is the first complete mitochondrial sequence of a species of the order Onygenales. This sequence is available under GenBank accession number AY916130.

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Abbreviations

bp:

base pairs

mtDNA:

mitochondrial DNA

rRNA:

ribosomal RNA

tRNA:

transfer RNA

ITS:

Internal transcribed spacer

Blast:

Basic local alignment search tool

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Acknowledgements

We thank Carolina Pomponio Barco for her essential contribution to the preparation of the libraries, Dr. Milton A. Typas for his help with proofreading the sequences and Dr. Walderez Gambale (Universidade de São Paulo) for providing the E. floccosum strain. Luis Gustavo Bueno Geraldo helped improve Fig. 1. We also acknowledge the support of Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).

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Authors and Affiliations

  1. Instituto de Ciências Biomédicas, Departamento de Microbiologia, Universidade de São Paulo, Av. Prof. Lineu Prestes, 1374, Ed. Biomédicas II, Cidade Universitária, CEP 05508-900, São Paulo, Brazil

    José Humberto M. Tambor

  2. Instituto de Pesquisa e Desenvolvimento, Universidade do Vale do Paraíba, Av. Shishima Hifumi, 2911, Urbanova, CEP 12244-000, São José dos Campos, Brazil

    José Humberto M. Tambor, Raquel F. Guedes, Marina P. Nobrega & Francisco G. Nobrega

  3. Laboratório de Genética Molecular e Genomas, IP&D, UNIVAP, Av. Shishima Hifumi 2911, Urbanova, 12244-000, São José dos Campos, São Paulo, Brazil

    Francisco G. Nobrega

Authors
  1. José Humberto M. Tambor
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  2. Raquel F. Guedes
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  3. Marina P. Nobrega
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  4. Francisco G. Nobrega
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Correspondence to Francisco G. Nobrega.

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Communicated by L. Tomaska

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Tambor, J.H.M., Guedes, R.F., Nobrega, M.P. et al. The complete DNA sequence of the mitochondrial genome of the dermatophyte fungus Epidermophyton floccosum . Curr Genet 49, 302–308 (2006). https://doi.org/10.1007/s00294-006-0057-2

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  • DOI: https://doi.org/10.1007/s00294-006-0057-2

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