Abstract
Entry into the stationary phase requires the yeast BCY1 gene, which encodes the regulatory subunit of the cAMP-dependent protein kinase (cAPK). New bcy1 mutants, constructed by in vitro mutagenesis of the 3′-region encoding the cAMP-binding domains, were classified as early or late-acting mutants based on viability studies. The late-acting bcy1 mutants accumulated fewer stationary phase-specific Bcy1p isoforms and had decreased cAPK activity. This late-acting class is novel and dies after 7 days in culture, later than two previously reported stationary phase mutants, ubi4 and ard1.
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Received: 4 November 1996 / 12 April 1997
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Peck, V., Fuge, E., Padilla, P. et al. Yeast bcy1 mutants with stationary phase-specific defects. Curr Genet 32, 83–92 (1997). https://doi.org/10.1007/s002940050251
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DOI: https://doi.org/10.1007/s002940050251