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Secondary DNA structure analysis of the coding strand switch regions of five Leishmania major Friedlin chromosomes

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Abstract.

As part of the EULEISH international genome project, a region of 74,674 nucleotides from chromosome 21 of Leishmania major Friedlin was subcloned and sequenced; and 31 new coding sequences were predicted. Of particular interest was a unique coding strand switching region covering 1.6 kb of DNA; and this was subjected to further investigation. Bioinformatic analysis of this region revealed an unusually high AT composition, a lack of putative hairpins and a strong curvature of the DNA in agreement with the structural characteristics of similar regions of other Leishmania chromosomes. These observations and a comparison with the secondary DNA structure of four other Leishmania chromosomes and chromosomes of different organisms could suggest a functional role of this region in transcription and mitotic division.

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Authors and Affiliations

  1. Microbiology Group, International Centre for Genetic Engineering and Biotechnology, AREA Science Park, Padriciano 99, 34012 Trieste, Italy, , , , ,

    Carlo V. Bruschi

  2. Department of Biology, Genomics Group, University of Trieste, Trieste, 34148, Italy, , , , ,

    Valentina Tosato & Laura Ciarloni

  3. The Sanger Centre, Hinxton, Cambridgeshire, CB10 1SA, UK, , , , ,

    Alasdair C. Ivens, Marie-Adèle Rajandream & Bart G. Barrell

Authors
  1. Valentina Tosato
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  2. Laura Ciarloni
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  3. Alasdair C. Ivens
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  4. Marie-Adèle Rajandream
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  5. Bart G. Barrell
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  6. Carlo V. Bruschi
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Tosato, V., Ciarloni, L., Ivens, A.C. et al. Secondary DNA structure analysis of the coding strand switch regions of five Leishmania major Friedlin chromosomes. Curr Genet 40, 186–194 (2001). https://doi.org/10.1007/s002940100246

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  • DOI: https://doi.org/10.1007/s002940100246

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