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The −844 G/A PAI-1 polymorphism is associated with mRNA expression in rheumatoid arthritis

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Abstract

We assessed whether the −844 G/A polymorphism and mRNA expression of plasminogen activator inhibitor 1 (PAI-1) gene are associated with rheumatoid arthritis (RA). Demographic data, hematological, biochemical parameters, disease activity–disability indexes, −844 G/A genotypes and mRNA expression levels of the PAI-1 gene were determined in 50 RA patients and 50 healthy subjects (HS). Non-significant differences in genotype and allele frequencies related to −844 G/A polymorphism in RA versus HS, were found. High mRNA expression of the PAI-1 gene, was demonstrated in RA versus HS (P < 0.05). In addition, A/A genotype carriers showed increase of PAI-1 mRNA expression (3.1-fold) respect to G/G and G/A genotypes in RA patients (P < 0.05). Our finding suggest an association of A/A −844 PAI-1 genotype with high PAI-1 mRNA expression in RA patients.

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Acknowledgments

This work was supported by grant no. 45703-M to JFMV of the National Council of Science and Technology (CONACyT, México-Universidad de Guadalajara). Preliminary results were presented in the Annual European Congress of Rheumatology, EULAR 2006 in Amsterdam, The Netherlands.

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Authors and Affiliations

  1. Instituto de Investigación en Reumatología y del Sistema Músculo Esquelético, Departamento de Biología Molecular y Genómica, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Sierra Mojada No. 950, Col. Independencia, P.O. Box 2-207, Guadalajara, Jalisco, 44340, Mexico

    Nora Magdalena Torres-Carrillo, Norma Torres-Carrillo, Mónica Vázquez-Del Mercado, Edith Oregón-Romero & José Francisco Muñoz-Valle

  2. Laboratorio de Inmunología, Departamento de Fisiología, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Sierra Mojada No. 950, Col. Independencia, Guadalajara, Jalisco, 44340, Mexico

    Vidal Delgado-Rizo

  3. Unidad Académica de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero. Av. Lázaro Cárdenas S/N, Ciudad Universitaria, Chilpancingo, Guerrero, Mexico

    Isela Parra-Rojas

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  1. Nora Magdalena Torres-Carrillo
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  2. Norma Torres-Carrillo
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  3. Mónica Vázquez-Del Mercado
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  4. Vidal Delgado-Rizo
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  5. Edith Oregón-Romero
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  6. Isela Parra-Rojas
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  7. José Francisco Muñoz-Valle
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Correspondence to José Francisco Muñoz-Valle.

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Torres-Carrillo, N.M., Torres-Carrillo, N., Vázquez-Del Mercado, M. et al. The −844 G/A PAI-1 polymorphism is associated with mRNA expression in rheumatoid arthritis. Rheumatol Int 28, 355–360 (2008). https://doi.org/10.1007/s00296-007-0453-z

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  • DOI: https://doi.org/10.1007/s00296-007-0453-z

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