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Comprehensive analysis of microRNA expression patterns in renal biopsies of lupus nephritis patients

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Abstract

MicroRNAs (miRNAs) are noncoding RNA molecules of 21–24 nt that regulate the expression of target genes in a post-transcriptional manner. Evidence indicates that miRNAs play essential roles in embryogenesis, cell differentiation, and pathogenesis of human diseases. This study describes a comparison between the miRNA profile of kidney biopsies from lupus nephritis (LN) patients and the controls, to develop further understanding of the pathogenesis of LN. Kidney biopsies were taken from five LN patients detected LN Class II and three normal controls. The miRNA microarray chip analysis identified 66 miRNAs differentially expressed in LN. The chip results were confirmed by QRT-PCR. This work indicates that miRNAs are potential diagnosis biomarkers and probable factors involved in the pathogenesis of LN.

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Acknowledgments

The authors thank the patients with LN and renal tumor who participated in this study.

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Authors and Affiliations

  1. The Second Clinical Medical College, Jinan University, Shenzhen People’s Hospital, 518020, Guangdong Province, China

    Yong Dai

  2. Kidney Transplantation and Hemo Purification Center, 181 Hospital of Guilin, 541000, Guangxi Province, China

    Weiguo Sui, Huijuan Lan, Qiang Yan & He Huang

  3. Key Laboratory of Laboratory Medical Diagnostics, Ministry of Education, Chongqing Medical University, 400016, Chongqing, China

    YuanShuai Huang

Authors
  1. Yong Dai
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  2. Weiguo Sui
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  3. Huijuan Lan
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  4. Qiang Yan
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  5. He Huang
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  6. YuanShuai Huang
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Corresponding author

Correspondence to YuanShuai Huang.

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Dai, Y., Sui, W., Lan, H. et al. Comprehensive analysis of microRNA expression patterns in renal biopsies of lupus nephritis patients. Rheumatol Int 29, 749–754 (2009). https://doi.org/10.1007/s00296-008-0758-6

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  • DOI: https://doi.org/10.1007/s00296-008-0758-6

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