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Plant regeneration from hairy-root cultures transformed by infection with Agrobacterium rhizogenes in Catharanthus roseus

  • Genetic Transformation and Hybridization
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Abstract

Hypocotyl explants of Catharanthus roseus produced hairy roots when cultured on Murashige and Skoog (MS) basal medium after infection by Agrobacterium rhizogenes. Explants gave rise to adventitious shoots at a frequency of up to 80% when cultured on MS medium supplemented with 31.1 μM 6-benzyladenine and 5.4 μM α-naphthaleneacetic acid. There was a significant difference in the frequency of adventitious shoot formation for each hairy-root line derived from a different cultivar. Plants derived from hairy roots exhibited prolific rooting and had shortened internodes. Approximately half of the plants had wrinkled leaves and an abundant root mass with extensive lateral branching, but otherwise appeared morphologically normal. Plants with hairy roots that were derived from the cultivar Cooler Apricot developed flowers with petals that were white in the proximal region, whereas the wild-type flower petals are red. PCR and Southern blot analyses revealed that plants derived from hairy roots retained the Ri TL-DNA.

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Fig. 1A-F
Fig. 2A–C

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Abbreviations

BA :

6-Benzyladenine

MS :

Murashige and Skoog medium

NAA :

α-Naphthaleneacetic acid

SH :

Schenk and Hildebrandt medium

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Acknowledgements

This work was supported by the following grants to J.R.L.: grant no. M10104000234-01J000-10710 from the National Research Laboratory Program; grant no. BDM 0100211 from the Strategic National R&D Program through the Genetic Resources and Information Network Center; a grant from the Korea Science and Engineering Foundation through the Plant Metabolism Research Center of Kyung Hee University funded by the Korean Ministry of Science and Technology; and a grant from the BioGreen 21 Program funded by the Korean Rural Development Administration.

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Correspondence to J.R. Liu.

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Communicated by I.S. Chung

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Choi, P., Kim, Y., Choi, K. et al. Plant regeneration from hairy-root cultures transformed by infection with Agrobacterium rhizogenes in Catharanthus roseus . Plant Cell Rep 22, 828–831 (2004). https://doi.org/10.1007/s00299-004-0765-3

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  • DOI: https://doi.org/10.1007/s00299-004-0765-3

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