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The rice pathogen-related protein 10 (JIOsPR10) is induced by abiotic and biotic stresses and exhibits ribonuclease activity

  • Biotic and Abiotic Stress
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An Erratum to this article was published on 03 September 2008

Abstract

We previously reported that rice blast fungus or jasmonic acid induced the expression of rice pathogenesis-related class 10 (JIOsPR10) proteins (Kim et al. 2003, 2004). However, no further studies have been carried out to examine the expression, localization, and enzymatic activity of this protein in either developmental tissues or in tissues under abiotic stress conditions. In this study, rice JIOsPR10 was examined by Western blot analysis, immunolocalization, and biochemical assays. Western blots revealed that the JIOsPR10 protein was expressed in developmental tissues, including in flower and root. The protein was also expressed under abiotic stresses, such as occurs during senescence and wounding. Using immunohistochemical techniques, we determined that expression of JIOsPR10 was localized to the palea of flower, in the exodermis, and inner part of the endodermis of the root. In senescencing tissues of leaf and coleoptiles, its expression was localized in vascular bundles. The RNase activity using JIOsPR10 recombinant protein was determined and abolished after treatment with DTT in a native in-gel assay. To test this, we created JIOsPR10 mutant proteins containing serine substitutions of amino acids C81S, C83S, or both and examined their RNase activities. The activity of the C83S mutant was decreased in the agarose gel assay compared to the wild type. Taken together, we hypothesize that the JIOsPR10 protein possesses RNase activity that is sensitive to DTT, suggesting the importance of the disulfide bonding between cysteine residues and that it might play a role in constitutive self-defense mechanisms in plants against biotic and abiotic stresses.

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Acknowledgments

We thank Dr. Hee Jong Ko, Seoul National University for kindly providing us with the lesion mimic mutants. This work was supported by ARPC, by a grant from KOSEF/MOST to the Environmental Biotechnology National Core Research Center and to National Research Laboratory on plant intercellular trafficking and by scholarships from the Brain Korea 21 Program.

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Authors and Affiliations

  1. Environmental Biotechnology National Core Research Center, Gyeongsang National University, Jinju, 660-701, Korea

    Sun Tae Kim, Jae-Yean Kim & Kyu Young Kang

  2. Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju, 660-701, Korea

    Seok Yu, Jae-Yean Kim & Kyu Young Kang

  3. Division of Applied Life Science (BK21 Program), Gyeongsang National University, Jinju, 660-701, Korea

    Young Hyun Kang, Sang Gon Kim, Jae-Yean Kim & Kyu Young Kang

  4. Department of Environmental Horticulture, University of Seoul, Seoul, 130-743, Korea

    Sun-Hyung Kim

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  1. Sun Tae Kim
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Correspondence to Kyu Young Kang.

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Communicated by H. Judelson.

An erratum to this article can be found at http://dx.doi.org/10.1007/s00299-008-0600-3

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Kim, S.T., Yu, S., Kang, Y.H. et al. The rice pathogen-related protein 10 (JIOsPR10) is induced by abiotic and biotic stresses and exhibits ribonuclease activity. Plant Cell Rep 27, 593–603 (2008). https://doi.org/10.1007/s00299-007-0485-6

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